BIOD 171 MODULE 3 EXAM QUESTIONS
AND ANSWERS
A vnanometer vis vdefined vas: v- vAnswer v- v10 v-9 vor vone-billionth vof va vmeter
True vor vFalse: vA vnanometer vis vlonger vthan va vmicrometer v- vAnswer v- vFalse
A vnanometer vis v1000x vsmaller vthan va vmicrometer
Resolution vand vcontrast vare vtwo vcritical vfactors vthat vinfluence vyour vability vto vsee van vobject.
vExplain veach. v- vAnswer v- vResolution vrefers v to v the vdistance vbetween vtwo vobjects vat vwhich v the
vobjects v can vstill vbe vseen vas vseparate. vPoor v or vlow vresolution vmeans vtwo vor vmore vobjects vmay
vappear vas vone.
Contrast von vthe vother vhand vis vthe vdifference vin vlight vabsorbance vbetween vtwo vobjects. vPoor
vcontrast vgives va vhigh vbackground vand vmakes vthe v visualization vof vmultiple vobjects vdifficult. vFor
vinstance, vtrying vto videntify v2 vdark vcolored vobjects vat
night v(low vlight v= vlow vcontrast) vversus vthe vsame v2 vobjects vin vthe vmiddle vof
a vsunny vafternoon v(bright vlight vagainst v2 vdark vobjects v= vhigh vcontrast).
Assuming va vfixed vocular, videntify vthe vpart vof vthe vmicroscope vyou vwould vadjust vto venhance vthe
vmagnification vof va vsample. v- vAnswer v- vObjective
Only vthe voculars v(eyepiece) vand vthe vobjectives vcontribute vto vthe vmagnification vof vthe vsample.
vSince vthe veyepiece
is vfixed, vonly vthe vobjectives vcould vbe valtered.
, Assuming va vconstant v(non-adjustable) vlight vsource vpower, videntify vthe vpart vof vthe vmicroscope
vyou vwould vadjust vto vlimit vthe vamount vof vlight ventering vthe vmicroscope. v- vAnswer v- vIris
vdiaphragm
What vis vthe vtotal vmagnification von v(relative vto vyour veye) vof va vsample vimaged vwith va v60x
vobjective vand va v10x veyepiece? vShow vyour vmath. v- vAnswer v- v60x vobjective vx v10x v ocular v= v 600x
vmagnification
True vor vFalse: vStaining vis voften vrequired vto vimage va vcell vthat vis vadherent vand vflat v(thin). v-
vAnswer v- vTrue
Adherent, vflat vand vunstained vcells vare valmost vinvisible vdue vto vthe vlimits von vboth
resolution vand vcontrast. vTherefore, vcell vstaining vis voften vrequired vto vadequately vimage
the vsample.
True vor vFalse: vA vcell vthat vis vadherent, vflat v(thin), vand vunstained vis veasily videntified vusing vbright
vfield vmicroscopy v- vAnswer v- vFalse: vAdherent, vflat v cells vare valmost vinvisible vdue vto vthe vlimits von
vboth vresolution vand v contrast
Which vof vthe vfollowing vcould vbe vseen vclearly vby vthe vunaided veye? vSelect vall vthat vapply.
A. vBacteria vwith vdiameter vof v24 vμm
B. vProtozoa vwith vdiameter vof v150 vμm
C. vVirus vwith va vdiameter vof v0.2 vμm
D. vSkin vcell vwith vdiameter vof v1500 vμm v- vAnswer v- vB. vProtozoa vwith vdiameter vof v150 vμm
D. vSkin vcell vwith vdiameter vof v1500 vμm
The vunaided veye vcan, von vaverage, vclearly vresolve vobjects v> v100 vμm
2. vWhich vof vthe vfollowing vcould vNOT vbe vseen vclearly vby vthe vunaided veye? vSelect vall vthat vapply.
AND ANSWERS
A vnanometer vis vdefined vas: v- vAnswer v- v10 v-9 vor vone-billionth vof va vmeter
True vor vFalse: vA vnanometer vis vlonger vthan va vmicrometer v- vAnswer v- vFalse
A vnanometer vis v1000x vsmaller vthan va vmicrometer
Resolution vand vcontrast vare vtwo vcritical vfactors vthat vinfluence vyour vability vto vsee van vobject.
vExplain veach. v- vAnswer v- vResolution vrefers v to v the vdistance vbetween vtwo vobjects vat vwhich v the
vobjects v can vstill vbe vseen vas vseparate. vPoor v or vlow vresolution vmeans vtwo vor vmore vobjects vmay
vappear vas vone.
Contrast von vthe vother vhand vis vthe vdifference vin vlight vabsorbance vbetween vtwo vobjects. vPoor
vcontrast vgives va vhigh vbackground vand vmakes vthe v visualization vof vmultiple vobjects vdifficult. vFor
vinstance, vtrying vto videntify v2 vdark vcolored vobjects vat
night v(low vlight v= vlow vcontrast) vversus vthe vsame v2 vobjects vin vthe vmiddle vof
a vsunny vafternoon v(bright vlight vagainst v2 vdark vobjects v= vhigh vcontrast).
Assuming va vfixed vocular, videntify vthe vpart vof vthe vmicroscope vyou vwould vadjust vto venhance vthe
vmagnification vof va vsample. v- vAnswer v- vObjective
Only vthe voculars v(eyepiece) vand vthe vobjectives vcontribute vto vthe vmagnification vof vthe vsample.
vSince vthe veyepiece
is vfixed, vonly vthe vobjectives vcould vbe valtered.
, Assuming va vconstant v(non-adjustable) vlight vsource vpower, videntify vthe vpart vof vthe vmicroscope
vyou vwould vadjust vto vlimit vthe vamount vof vlight ventering vthe vmicroscope. v- vAnswer v- vIris
vdiaphragm
What vis vthe vtotal vmagnification von v(relative vto vyour veye) vof va vsample vimaged vwith va v60x
vobjective vand va v10x veyepiece? vShow vyour vmath. v- vAnswer v- v60x vobjective vx v10x v ocular v= v 600x
vmagnification
True vor vFalse: vStaining vis voften vrequired vto vimage va vcell vthat vis vadherent vand vflat v(thin). v-
vAnswer v- vTrue
Adherent, vflat vand vunstained vcells vare valmost vinvisible vdue vto vthe vlimits von vboth
resolution vand vcontrast. vTherefore, vcell vstaining vis voften vrequired vto vadequately vimage
the vsample.
True vor vFalse: vA vcell vthat vis vadherent, vflat v(thin), vand vunstained vis veasily videntified vusing vbright
vfield vmicroscopy v- vAnswer v- vFalse: vAdherent, vflat v cells vare valmost vinvisible vdue vto vthe vlimits von
vboth vresolution vand v contrast
Which vof vthe vfollowing vcould vbe vseen vclearly vby vthe vunaided veye? vSelect vall vthat vapply.
A. vBacteria vwith vdiameter vof v24 vμm
B. vProtozoa vwith vdiameter vof v150 vμm
C. vVirus vwith va vdiameter vof v0.2 vμm
D. vSkin vcell vwith vdiameter vof v1500 vμm v- vAnswer v- vB. vProtozoa vwith vdiameter vof v150 vμm
D. vSkin vcell vwith vdiameter vof v1500 vμm
The vunaided veye vcan, von vaverage, vclearly vresolve vobjects v> v100 vμm
2. vWhich vof vthe vfollowing vcould vNOT vbe vseen vclearly vby vthe vunaided veye? vSelect vall vthat vapply.
