BIOD 171: MODULE 4 EXAM WITH
CORRECT QUESTIONS AND ANSWERS
2025
T/F: Growth media is best suited for distinguishing between two similar species of
bacteria - CORRECT-ANSWERSFalse
growth media is designed to simply support (and not restrict) microbial growth
A researcher is asked to determine which of two vials contains E coli and which contains
salmonella. Knowing both are Gram-negative while only one of them is capable of
fermenting lactose, which type of media would be best suited? - CORRECT-
ANSWERSDifferential media
What are the requirements of a fastidious microbe? - CORRECT-ANSWERSA fastidious
microbe is an organism with complex growth requirements and will not grow without
then. Enriched medias thus contain these specific and essential nutrients required for the
growth of a particular subset of microorganisms.
T/F: LB agar is classified as a non-selective, non-differential media - CORRECT-
ANSWERSTrue
The polysaccharide-based hardening agent added to LB media to produce LB agar is
derived from what? - CORRECT-ANSWERSSeaweed extract
Blood agar is which type of medium? Select all that apply:
- Selective and Differential
- Enriched
, - Differential
- Selective - CORRECT-ANSWERS- Enriched
- Differential
Match the following hemolytic class with its description of activity:
Alpha hemolysis
Beta hemolysis
Gamma hemolysis
- No change
- Greenish-brown color
- Distinct zone of clearing - CORRECT-ANSWERS1. Alpha hemolysis- Greenish-
brown color (B)
2. Beta hemolysis- Distinct zone of clearing (C)
Gamma hemolysis- No change (A)
Match the following hemolytic class with its description of activity:
Alpha hemolysis
Beta hemolysis
Gamma hemolysis
- No hemolytic activity
- Incomplete hemolytic activity
CORRECT QUESTIONS AND ANSWERS
2025
T/F: Growth media is best suited for distinguishing between two similar species of
bacteria - CORRECT-ANSWERSFalse
growth media is designed to simply support (and not restrict) microbial growth
A researcher is asked to determine which of two vials contains E coli and which contains
salmonella. Knowing both are Gram-negative while only one of them is capable of
fermenting lactose, which type of media would be best suited? - CORRECT-
ANSWERSDifferential media
What are the requirements of a fastidious microbe? - CORRECT-ANSWERSA fastidious
microbe is an organism with complex growth requirements and will not grow without
then. Enriched medias thus contain these specific and essential nutrients required for the
growth of a particular subset of microorganisms.
T/F: LB agar is classified as a non-selective, non-differential media - CORRECT-
ANSWERSTrue
The polysaccharide-based hardening agent added to LB media to produce LB agar is
derived from what? - CORRECT-ANSWERSSeaweed extract
Blood agar is which type of medium? Select all that apply:
- Selective and Differential
- Enriched
, - Differential
- Selective - CORRECT-ANSWERS- Enriched
- Differential
Match the following hemolytic class with its description of activity:
Alpha hemolysis
Beta hemolysis
Gamma hemolysis
- No change
- Greenish-brown color
- Distinct zone of clearing - CORRECT-ANSWERS1. Alpha hemolysis- Greenish-
brown color (B)
2. Beta hemolysis- Distinct zone of clearing (C)
Gamma hemolysis- No change (A)
Match the following hemolytic class with its description of activity:
Alpha hemolysis
Beta hemolysis
Gamma hemolysis
- No hemolytic activity
- Incomplete hemolytic activity