mbg exam 3 questions with verified answers graded
A+ updated to pass
1. it is typical in
animal models are a great method of studying gene function
med- icine to
utilize ani- mal
models to clini-
cally test
treatments. why?
2. the zebra fish, Danio rerio
sci- entifically
known as
, is an an- used for mapping & identifying genes involved in organ
development
imal model that
is used for
what?
3. what made it knock-
possi- ble for
animal mod- els
to be utilized
to study human
dis- eases?
4. explain the
trans- genic
mice animal
model.
5. explain the
,mbg exam 3 questions with verified answers graded
A+ updated to pass
knockout technology
normal human gene is inserted into a mouse egg with normal mous
this technology allowed karyotype
scientists to create animal
models of human disease in you add a normal human gene & observe the gene product in the
mice
mice you introduce a mutation that suppresses expression of one
of the mouse's
out/knockin mice an- genes
imal model.
mutated gene is then inserted into the mouse genome via
homologous recombination
,mbg exam 3 questions with verified answers graded
A+ updated to pass
you then observe the phenotype that results from knocking out the
original gene
6. what is a transgene? gene that has been microinjected into pronucleus of a mouse
embryo
7. which imaging PCR or southern blot
tech- nique is
utilized to
observe if
microin- jected
transgenes are
present in a
mouse embryo? head to tail concatemers
8. transgenes are
integrated into
mouse embryos
as
9. what are the pronuclear injection
two ways microinject human DNA into the pronucleus of a mouse embryo ; the
transgenic mice now termed transgene will randomly integrate into chromosomal
can be made?
DNA of the mouse embryo
insertion of transgene into ES cells
DNA is inserted into embryonic stem (ES) cell, then we insert those E
cells into mouse embryo
,mbg exam 3 questions with verified answers graded
A+ updated to pass
A+ updated to pass
1. it is typical in
animal models are a great method of studying gene function
med- icine to
utilize ani- mal
models to clini-
cally test
treatments. why?
2. the zebra fish, Danio rerio
sci- entifically
known as
, is an an- used for mapping & identifying genes involved in organ
development
imal model that
is used for
what?
3. what made it knock-
possi- ble for
animal mod- els
to be utilized
to study human
dis- eases?
4. explain the
trans- genic
mice animal
model.
5. explain the
,mbg exam 3 questions with verified answers graded
A+ updated to pass
knockout technology
normal human gene is inserted into a mouse egg with normal mous
this technology allowed karyotype
scientists to create animal
models of human disease in you add a normal human gene & observe the gene product in the
mice
mice you introduce a mutation that suppresses expression of one
of the mouse's
out/knockin mice an- genes
imal model.
mutated gene is then inserted into the mouse genome via
homologous recombination
,mbg exam 3 questions with verified answers graded
A+ updated to pass
you then observe the phenotype that results from knocking out the
original gene
6. what is a transgene? gene that has been microinjected into pronucleus of a mouse
embryo
7. which imaging PCR or southern blot
tech- nique is
utilized to
observe if
microin- jected
transgenes are
present in a
mouse embryo? head to tail concatemers
8. transgenes are
integrated into
mouse embryos
as
9. what are the pronuclear injection
two ways microinject human DNA into the pronucleus of a mouse embryo ; the
transgenic mice now termed transgene will randomly integrate into chromosomal
can be made?
DNA of the mouse embryo
insertion of transgene into ES cells
DNA is inserted into embryonic stem (ES) cell, then we insert those E
cells into mouse embryo
,mbg exam 3 questions with verified answers graded
A+ updated to pass
