Midterm Test Bank (microbiology)
With Correct Answers Graded A+
Place the steps of the gram stain in the correct order: 1-alcohol-acetone; 2-crystal
violet 3-safranin; 4-iodine.
2, 4, 1, 3
Which of the following organelles most closely resembles a prokaryotic cell?
mitochondrion
Which of the following is NOT a product of transcription?
a new strand of DNA
E. coli makes insulin because
the insulin gene was inserted into it
This microscope produces an image of a light cell against a dark background;
internal structures are NOT visible.
darkfield microscope
The appearance of gram-negative bacteria after addition of the mordant in the
Gram stain.
Colorless
PCR can be used to amplify DNA in a clinical sample. The following steps are
used in PCR. What is the fourth step?
Incubate at 60 degrees Celsius
The appearance of gram-positive bacteria after addition of the first dye in the
Gram stain
purple
Who proved that microorganisms cause disease?
Pasteur
In Figure 6.1 ,which line best depicts an obligate anaerobe in the presence of O2?
Line C
Which microscope is used to observe a specimen that emits light when
illuminated with an ultraviolet light?
fluorescence microscope
A gene is best defined as
a sequence of nucleotides in DNA that codes for a functional product
You want to determine whether a person has a certain mutant gene. The process
involves using a primer and TAQ. This process is
PCR
Which of the following structures is NOT found in prokaryotic cells?
Cillium
Which of the following does NOT achieve sterilization?
Pasteurization
A strictly fermentative bacterium produces energy
Only in the presence of oxygen
With Correct Answers Graded A+
Place the steps of the gram stain in the correct order: 1-alcohol-acetone; 2-crystal
violet 3-safranin; 4-iodine.
2, 4, 1, 3
Which of the following organelles most closely resembles a prokaryotic cell?
mitochondrion
Which of the following is NOT a product of transcription?
a new strand of DNA
E. coli makes insulin because
the insulin gene was inserted into it
This microscope produces an image of a light cell against a dark background;
internal structures are NOT visible.
darkfield microscope
The appearance of gram-negative bacteria after addition of the mordant in the
Gram stain.
Colorless
PCR can be used to amplify DNA in a clinical sample. The following steps are
used in PCR. What is the fourth step?
Incubate at 60 degrees Celsius
The appearance of gram-positive bacteria after addition of the first dye in the
Gram stain
purple
Who proved that microorganisms cause disease?
Pasteur
In Figure 6.1 ,which line best depicts an obligate anaerobe in the presence of O2?
Line C
Which microscope is used to observe a specimen that emits light when
illuminated with an ultraviolet light?
fluorescence microscope
A gene is best defined as
a sequence of nucleotides in DNA that codes for a functional product
You want to determine whether a person has a certain mutant gene. The process
involves using a primer and TAQ. This process is
PCR
Which of the following structures is NOT found in prokaryotic cells?
Cillium
Which of the following does NOT achieve sterilization?
Pasteurization
A strictly fermentative bacterium produces energy
Only in the presence of oxygen
