,91 Radioimmunoassay in
.
thyroid abnormalites
RIA is used
for estimation
of hormones proteins drugs
,
·
, ,
and vitamins in like wine I
-
body funds plasma ,
Of .
It works on the basis
of competetive binding
·
components especific (against hormone to be assayed)
As
·
Radioactively labered hormone
.
·
It is labelled with -
peptide hormone : radioactive iodine
↳ steroid hormone :
Carbon , Hydrogen
Steps : -
Radioactive hormone is added to known amount
of
Ab
↓
Sample hormone competes with radioactive hormone
Ab
for binding
d
Bound radioactivity is measured
In inyroid hormone
:
-
B 4 are usually measured
by RIA
·
.
·
P Hyperthyroidism Thyroid : hormones 4
(feedback untibution)
TSHA
1"Hypothyroidism Thyroid hormones I
·
:
TSH4
·
Pitary hyperthyroidism :
Ts T TSH4
, ,
, G Polyphagia
. & Polydypsia in DM
MM is characterised
by polyphagia , polydipsia
, polyuria
,
weight loss in spite of food intake Hyperglycemia I
,
glycosia
Polyphagia
Glucose uptake into cells of brain (exc VMH) is independent
·
of insulin
.
VMH
depends on insulin for glu uptakeI utilisation ,
·
-
insulin
deficiency in DM & activity of VMH.
↓
-
↓ VMH activity removes inhibitory
effect on HH.
d
I activity due to disintuition
↓
This causes facilitation of feeding centre
↓
Polyphagia
Polyuria
Plasma increases
glucose about renal threshold
(180mgr),
·
so
gu appears in urine
osmotic diuresis
Increased
glu in turnar fluid causes
·
(water followes glu)
Polydipsia
·
Water is lost in excess due to polyuria decreasing
,
&ECF
plasma volumes.
·
Dehydration stimmates thirst centre
, 9 Cretinism .
·
When hypothyroidism develops from or
before birth
causes -
:
·
Maternal iodine deficiency during pregnancy
·
Maldevelopment of Thyroid gland during IVL .
Inborn errors
of thyroid hormone synthesis
·
.
·
Antithyroid Ab (maternal) crossing placenta
·
Hypopituitarism
Features :
-
⑧Ham a -
retard
,
way
·
dwarf tongue
·
mentally retarded Pot belly
potbelly Menorrhagia
#
·
·
protrusion of tongue
other
features of hypothyroidism
·
·
cold intolerance
IIIS
weakness & fatigue
·
·
dry ,
thick skin
·
hair loss , alopecia
·
poor memory
·
inability to concentrate
·
-- weight gain
consupation
·
thick I husky voice
·
yellow skin (thyronine causes arotene - VitA)
S
psychoses
·
-
mennornagea
·
S
·
unfertility
.
thyroid abnormalites
RIA is used
for estimation
of hormones proteins drugs
,
·
, ,
and vitamins in like wine I
-
body funds plasma ,
Of .
It works on the basis
of competetive binding
·
components especific (against hormone to be assayed)
As
·
Radioactively labered hormone
.
·
It is labelled with -
peptide hormone : radioactive iodine
↳ steroid hormone :
Carbon , Hydrogen
Steps : -
Radioactive hormone is added to known amount
of
Ab
↓
Sample hormone competes with radioactive hormone
Ab
for binding
d
Bound radioactivity is measured
In inyroid hormone
:
-
B 4 are usually measured
by RIA
·
.
·
P Hyperthyroidism Thyroid : hormones 4
(feedback untibution)
TSHA
1"Hypothyroidism Thyroid hormones I
·
:
TSH4
·
Pitary hyperthyroidism :
Ts T TSH4
, ,
, G Polyphagia
. & Polydypsia in DM
MM is characterised
by polyphagia , polydipsia
, polyuria
,
weight loss in spite of food intake Hyperglycemia I
,
glycosia
Polyphagia
Glucose uptake into cells of brain (exc VMH) is independent
·
of insulin
.
VMH
depends on insulin for glu uptakeI utilisation ,
·
-
insulin
deficiency in DM & activity of VMH.
↓
-
↓ VMH activity removes inhibitory
effect on HH.
d
I activity due to disintuition
↓
This causes facilitation of feeding centre
↓
Polyphagia
Polyuria
Plasma increases
glucose about renal threshold
(180mgr),
·
so
gu appears in urine
osmotic diuresis
Increased
glu in turnar fluid causes
·
(water followes glu)
Polydipsia
·
Water is lost in excess due to polyuria decreasing
,
&ECF
plasma volumes.
·
Dehydration stimmates thirst centre
, 9 Cretinism .
·
When hypothyroidism develops from or
before birth
causes -
:
·
Maternal iodine deficiency during pregnancy
·
Maldevelopment of Thyroid gland during IVL .
Inborn errors
of thyroid hormone synthesis
·
.
·
Antithyroid Ab (maternal) crossing placenta
·
Hypopituitarism
Features :
-
⑧Ham a -
retard
,
way
·
dwarf tongue
·
mentally retarded Pot belly
potbelly Menorrhagia
#
·
·
protrusion of tongue
other
features of hypothyroidism
·
·
cold intolerance
IIIS
weakness & fatigue
·
·
dry ,
thick skin
·
hair loss , alopecia
·
poor memory
·
inability to concentrate
·
-- weight gain
consupation
·
thick I husky voice
·
yellow skin (thyronine causes arotene - VitA)
S
psychoses
·
-
mennornagea
·
S
·
unfertility
