BISC 220 Week 1 Exam Questions With
100% Verified Answers!!
What is paper chromatography used for? How does it work?
Paper chromatography is a technique used to separate chlorophyll and other plant pigments
based on their solubility in a solvent. The process involves extracting pigments from
leaves, applying the extract to chromatography paper, and allowing a solvent to travel up
the paper, separating the different pigments. The pigments travel different distances based
on their solubility in the solvent, resulting in distinct bands or spots on the paper.
On a paper chromatography where is the solvent front, the pigment origin, and
solvent origin?
Solvent origin (bottom most), pigment origin (just above solvent origin), solvent front
(furthest point solvent reaches
Distance travelled used to calculate Rf
Why does solute separate from solvent in paper chromatography?
In paper chromatography, separation of a mixture occurs because different components have
varying attractions to both the stationary phase (the paper) and the mobile phase (the solvent).
This difference in attraction, which is related to the component's solubility and polarity, leads
to them moving at different speeds across the paper, effectively separating them
What is the Rf value?
Rf value is a measure of retention, and tells you the purity of the solute based on distance
travelled (closer is more pure) and chemical behavior
What is the formula for Rf value?
Distance moved by solute / distance moved by solvent (solvent front)
, How do you make gel agarose?
To create an agarose gel, you dissolve agarose powder in a buffer and heat it until it melts. Once
cooled, the solution is poured into a casting tray, where it solidifies, forming a gel with tiny
pores. This gel acts as a molecular sieve in gel electrophoresis, separating DNA fragments based
on their size
When you use enzymes to digest a plasmid, how can you calculate the size of each segment?
Find the start and end point, subtract (pay attention to number of segments)
What are sticky vs blunt ends of DNA?
Sticky = uneven = binds easy
Blunt = even = doesn't bing
What are the main parts of the brain?
Right and left hemisphere
(Front to back)
Frontal lobe, parietal lobe, occipital lobe (back) temporal lobe (front), cerebellum, spinal cord
What is the cerebrum?
Largest part of brain, divided into two hemispheres
What is the function of the brain and spinal cord?
Central nervous system
Brain process info and send signals
- cerebrum (thinking / senses)
- cerebellum (movement and balance)
- brain stem (basic life functions + reflexes)
What does the frontal lobe control?
100% Verified Answers!!
What is paper chromatography used for? How does it work?
Paper chromatography is a technique used to separate chlorophyll and other plant pigments
based on their solubility in a solvent. The process involves extracting pigments from
leaves, applying the extract to chromatography paper, and allowing a solvent to travel up
the paper, separating the different pigments. The pigments travel different distances based
on their solubility in the solvent, resulting in distinct bands or spots on the paper.
On a paper chromatography where is the solvent front, the pigment origin, and
solvent origin?
Solvent origin (bottom most), pigment origin (just above solvent origin), solvent front
(furthest point solvent reaches
Distance travelled used to calculate Rf
Why does solute separate from solvent in paper chromatography?
In paper chromatography, separation of a mixture occurs because different components have
varying attractions to both the stationary phase (the paper) and the mobile phase (the solvent).
This difference in attraction, which is related to the component's solubility and polarity, leads
to them moving at different speeds across the paper, effectively separating them
What is the Rf value?
Rf value is a measure of retention, and tells you the purity of the solute based on distance
travelled (closer is more pure) and chemical behavior
What is the formula for Rf value?
Distance moved by solute / distance moved by solvent (solvent front)
, How do you make gel agarose?
To create an agarose gel, you dissolve agarose powder in a buffer and heat it until it melts. Once
cooled, the solution is poured into a casting tray, where it solidifies, forming a gel with tiny
pores. This gel acts as a molecular sieve in gel electrophoresis, separating DNA fragments based
on their size
When you use enzymes to digest a plasmid, how can you calculate the size of each segment?
Find the start and end point, subtract (pay attention to number of segments)
What are sticky vs blunt ends of DNA?
Sticky = uneven = binds easy
Blunt = even = doesn't bing
What are the main parts of the brain?
Right and left hemisphere
(Front to back)
Frontal lobe, parietal lobe, occipital lobe (back) temporal lobe (front), cerebellum, spinal cord
What is the cerebrum?
Largest part of brain, divided into two hemispheres
What is the function of the brain and spinal cord?
Central nervous system
Brain process info and send signals
- cerebrum (thinking / senses)
- cerebellum (movement and balance)
- brain stem (basic life functions + reflexes)
What does the frontal lobe control?
