sh
ne
BIOD 171 EXAM MODULE 4 la
tz
True or False: Growth media is best suited for distinguishing between two similar species of bacteria.
sh
Sp
ne
False. Growth media is designed to simply support (and not restrict) microbial growth.
la
sh
Sp
ne
2. A researcher is asked to determine which of two vials contains E coli and which contains
la
Salmonella. Knowing both are Gram-Negative while only one of them is capable of fermenting
tz
lactose, which type of media would be best suited:
sh
Sp
ne
la
sh
Sp
ne
la
tz
sh
Sp
ne
la
sh
Sp
ne
la
tz
sh
Sp
ne
la
sh
Sp
ne
a
tz
l
sh
Sp
ne
la
sh
Sp
la
Sp
, ne
sh
ne
A. Growth media
B. Differential media
la
tz
C. Selective media
sh
Sp
D. Selective and Differential media
ne
la
B. Differential media distinguishes between two, often related, microbes.
sh
Sp
ne
3. What are the requirements of a fastidious microbe?
la
tz
sh
Sp
ne
A fastidious microbe is an organism with complex growth requirements such that if absent itwill not grow.
Enriched medias thus contain these specific and essential nutrients required for the growth of a particular
Exam Page 2
la
subset of microorganisms.
sh
Answer Key
Sp
ne
1. True or False: LB agar is classified as a selective, non-differential media.
la
tz
False. LB agar is the most basic type of agar and like LB media supports the growth ofvirtually all microbes
sh
Sp
without restriction.
ne
la
sh
2. What is agar used for in microbiology?
Sp
ne
Exam
Agar is usedPage
to create a3
la
solid, smooth surface on which microbes can grow.
tz
Answer Key
sh
Sp
ne
1. Match the following hemolytic class with its description of activity.
la
sh
Sp
ne
1. Alpha hemolysis
a
B A. No hemolytic activity
tz
l
sh
Sp
2. Beta hemolysis
ne
C B. Incomplete hemolytic activity
la
sh
Sp
la
Sp