Bios 1705 Lab Exam 3 2025/2026 Exam
Questions and Verified Answers |
1
Already Graded A+
How does bacteria replicate? - 🧠 ANSWER ✔✔Asexually by binary fission:
ability to incorporate new genetic material from surroundings
What is transformation? - 🧠 ANSWER ✔✔ability to bind naked DNA in the
environment and absorb it ?
What type of DNA does E.Coli have? - 🧠 ANSWER ✔✔Circular DNA
What are plasmids? - 🧠 ANSWER ✔✔Small circular DNA molecules
What does extra-chromosomal mean? - 🧠 ANSWER ✔✔they are not a part
of the bacterial genome
What is bio luminescent? - 🧠 ANSWER ✔✔to glow or produce light
What is pGreen? - 🧠 ANSWER ✔✔plasmid which has a gene for ampr and
a gene for GFP
COPYRIGHT©PROFFKERRYMARTIN 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
PRIVACY STATEMENT. ALL RIGHTS RESERVED
, Bacteria are grown in? - 🧠 ANSWER ✔✔Media
Where are bacteria easily grown? - 🧠 ANSWER ✔✔In LB broth and or agar
2
What is competent? - 🧠 ANSWER ✔✔ability to take up the plasmid
What is chemical competancy - 🧠 ANSWER ✔✔induced in E.Coli by testing
with chloride salts of metal cation calcium,magnesium and rubidium.
Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency? - 🧠 ANSWER
✔✔disrupts the bacterial cell and membrane to create small holes in which
DNA enters
What is electroporation? - 🧠 ANSWER ✔✔electric pulses which transiently
changes the cell membrane structure and allows foreign DNA into the cell
without killing most of the cells
ampr - 🧠 ANSWER ✔✔commonly used gene when transforming bacteria
Primers are needed for? - 🧠 ANSWER ✔✔amplification of specific parts of
DNA
Transformation efficiency - 🧠 ANSWER ✔✔refers to the number of cells
that were transformed per micro gram of plasmid DNA
COPYRIGHT©PROFFKERRYMARTIN 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
PRIVACY STATEMENT. ALL RIGHTS RESERVED
Questions and Verified Answers |
1
Already Graded A+
How does bacteria replicate? - 🧠 ANSWER ✔✔Asexually by binary fission:
ability to incorporate new genetic material from surroundings
What is transformation? - 🧠 ANSWER ✔✔ability to bind naked DNA in the
environment and absorb it ?
What type of DNA does E.Coli have? - 🧠 ANSWER ✔✔Circular DNA
What are plasmids? - 🧠 ANSWER ✔✔Small circular DNA molecules
What does extra-chromosomal mean? - 🧠 ANSWER ✔✔they are not a part
of the bacterial genome
What is bio luminescent? - 🧠 ANSWER ✔✔to glow or produce light
What is pGreen? - 🧠 ANSWER ✔✔plasmid which has a gene for ampr and
a gene for GFP
COPYRIGHT©PROFFKERRYMARTIN 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
PRIVACY STATEMENT. ALL RIGHTS RESERVED
, Bacteria are grown in? - 🧠 ANSWER ✔✔Media
Where are bacteria easily grown? - 🧠 ANSWER ✔✔In LB broth and or agar
2
What is competent? - 🧠 ANSWER ✔✔ability to take up the plasmid
What is chemical competancy - 🧠 ANSWER ✔✔induced in E.Coli by testing
with chloride salts of metal cation calcium,magnesium and rubidium.
Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency? - 🧠 ANSWER
✔✔disrupts the bacterial cell and membrane to create small holes in which
DNA enters
What is electroporation? - 🧠 ANSWER ✔✔electric pulses which transiently
changes the cell membrane structure and allows foreign DNA into the cell
without killing most of the cells
ampr - 🧠 ANSWER ✔✔commonly used gene when transforming bacteria
Primers are needed for? - 🧠 ANSWER ✔✔amplification of specific parts of
DNA
Transformation efficiency - 🧠 ANSWER ✔✔refers to the number of cells
that were transformed per micro gram of plasmid DNA
COPYRIGHT©PROFFKERRYMARTIN 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
PRIVACY STATEMENT. ALL RIGHTS RESERVED
