UNE Medical Biochemistry
UNE Medical Biochemistry Midterm Exam
Questions and Answers 2025
Which of the following proteins is likely to have quaternary structure? -Correct Answer
✔A multimeric protein that contains multiple peptide chains
Prosthetic groups are complex nonprotein molecules that participate in catalysis by
providing functional groups that form a covalent intermediate between the enzyme and
the substrate. Which of the following is an example of a coenzyme or cofactor that
participates in a covalent catalysis reaction? -Correct Answer ✔Pyridoxal phosphate
Which of the following best describes a protein domain? -Correct Answer ✔A section of
a protein structure sufficient to perform a particular chemical or physical task
Cleavage of fructose 1,6 bisphosphate to dihydroxyacetone and Glyceraldehyde 3-
phosphate involves the breaking of a carbon-carbon bond through a process other than
oxidation or hydrolysis. This is achieved by what class of enzymes? -Correct Answer
✔Lyase
A 55-year old man is brought to the emergency room with a chief complaint of chest
pain and tingling in his left arm. Elevation of which of the following enzymes in
circulation would be most indicative of myocardial infarction? -Correct Answer
✔Troponin
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? -Correct Answer ✔Binding the protein, and keeping it in the "R"
conformation
UNE Medical Biochemistry
, UNE Medical Biochemistry
Which of the following best describes tertiary structure? -Correct Answer ✔Interactions
between alpha helices and beta sheets to form a domain
How do most enzymes reduce the activation energy needed to move a reaction
forward? -Correct Answer ✔providing an active site most complementary to the
transition state
Domains are common structural elements that retain a particular function within the
protein. Which of the following is an example of a protein domain? -Correct Answer
✔An arrangement of beta-strands connected by alpha-helices (beta-alpha-beta-alpha)
Which amino acid would not be commonly found in the middle of an alpha-helical
domain? -Correct Answer ✔Proline
Which of the following is a true statement with regards to the malate-aspartate shuttle
system? -Correct Answer ✔Generates 2.5 molecules of ATP in mitochondria per each
NADH from glycolysis.
Complex II (Succinate Q-reductase) is not considered part of the process of oxidative
phosphorylation because it is unable to do which of the following activities? -Correct
Answer ✔Pump protons from the mitochondrial matrix into the inter membrane space.
Succinate is a substrate for which complex in the electron transport chain? -Correct
Answer ✔Complex II (Succinate-Q reductase)
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the
following best describes the transfer of electrons by this shuttle? -Correct Answer
✔Electrons are directly transferred from glycerol 3-phosphate to an FAD containing
glycerol phosphate dehydrogenase.
UNE Medical Biochemistry
UNE Medical Biochemistry Midterm Exam
Questions and Answers 2025
Which of the following proteins is likely to have quaternary structure? -Correct Answer
✔A multimeric protein that contains multiple peptide chains
Prosthetic groups are complex nonprotein molecules that participate in catalysis by
providing functional groups that form a covalent intermediate between the enzyme and
the substrate. Which of the following is an example of a coenzyme or cofactor that
participates in a covalent catalysis reaction? -Correct Answer ✔Pyridoxal phosphate
Which of the following best describes a protein domain? -Correct Answer ✔A section of
a protein structure sufficient to perform a particular chemical or physical task
Cleavage of fructose 1,6 bisphosphate to dihydroxyacetone and Glyceraldehyde 3-
phosphate involves the breaking of a carbon-carbon bond through a process other than
oxidation or hydrolysis. This is achieved by what class of enzymes? -Correct Answer
✔Lyase
A 55-year old man is brought to the emergency room with a chief complaint of chest
pain and tingling in his left arm. Elevation of which of the following enzymes in
circulation would be most indicative of myocardial infarction? -Correct Answer
✔Troponin
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? -Correct Answer ✔Binding the protein, and keeping it in the "R"
conformation
UNE Medical Biochemistry
, UNE Medical Biochemistry
Which of the following best describes tertiary structure? -Correct Answer ✔Interactions
between alpha helices and beta sheets to form a domain
How do most enzymes reduce the activation energy needed to move a reaction
forward? -Correct Answer ✔providing an active site most complementary to the
transition state
Domains are common structural elements that retain a particular function within the
protein. Which of the following is an example of a protein domain? -Correct Answer
✔An arrangement of beta-strands connected by alpha-helices (beta-alpha-beta-alpha)
Which amino acid would not be commonly found in the middle of an alpha-helical
domain? -Correct Answer ✔Proline
Which of the following is a true statement with regards to the malate-aspartate shuttle
system? -Correct Answer ✔Generates 2.5 molecules of ATP in mitochondria per each
NADH from glycolysis.
Complex II (Succinate Q-reductase) is not considered part of the process of oxidative
phosphorylation because it is unable to do which of the following activities? -Correct
Answer ✔Pump protons from the mitochondrial matrix into the inter membrane space.
Succinate is a substrate for which complex in the electron transport chain? -Correct
Answer ✔Complex II (Succinate-Q reductase)
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the
following best describes the transfer of electrons by this shuttle? -Correct Answer
✔Electrons are directly transferred from glycerol 3-phosphate to an FAD containing
glycerol phosphate dehydrogenase.
UNE Medical Biochemistry
