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Lab 1 INCLUDE
What three elements are used in an au-
heat, pressure, and steam
toclave to sterilize equipment?
What is the minimum temperature an au-
toclave must be set at to achieve sterile 125°C
condition?
Fixed incubator
What type of incubator is pictured be-
low?
If you are working in a lab in which
an autoclave is not available, and you Hot steam is the best choice as you can
are pressed for time, which would you achieve a sterile environment in a matter
choose to best sterilize your equipment? of minutes whereas hot air will take sev-
Hot steam or hot air? Explain why you eral hours to achieve the same effect.
chose your answer.
What are the main sections that should
Objective, Procedure, Notes, Results
be found in a lab notebook? Name at
and Deviations
least 4.
At what temperature should you refriger- 4°C. This temperature slows bacterial
ate bacterial samples? Explain why this growth and prolongs the life of the sam-
is beneficial. ple.
What are the FOUR types of gloves pre- Latex, Nitrile, Thermal cold, Thermal
sented in the lab video? heat
At what temperature is the fixed incuba-
37°C
tor set to, as presented in the lab video?
What THREE rules were discussed in 1. Never eat or drink in the lab2. Always
regards to lab safety that would protect wear appropriate PPE3. Never leave the
you and others from contamination? lab wearing PPE
You are a lab instructor, and Sally Miller
has turned in her lab notebook for you
, Microbiology Final- Labs Verified Multiple Choice and Conceptual Actual Exam
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to grade the lab experiment #1 on lab
safety. Based upon what was covered
SM01 Lab Safety
in the lab video, how should Sally have
titled her experiment?
You arrive to your first day of work at a
new lab. You are taking over for some-
one who took a new job at another lab.
Your boss informs you that because of
time restraints, this person did not ex-
(1) Procedure—this is where the steps
actly follow the experimental protocol. In
for the experimental protocol are record-
order to proceed, you must know what
ed.(2) Deviations. All deviations should
he did differently. (1) According to the
be written in red to immediately bring
lab lecture, under what section of his
attention to the changes in the protocol.
notebook would you look to find the ex-
perimental steps? (2) As changes to the
experimental steps were made, what are
these differences called, and how should
it appear in the lab notebook?
Lab 2
Oculars/eyepiece
1. Identify the part of the microscope in-
dicated by the arrow.
objectives/objective lens
1. Identify the part of the microscope in-
dicated by the arrow.
coarse focus
1. Identify the part of the microscope in-
dicated by the arrow.