GMS 6504 MODULE 1-3 Exam 1(actual 2025)
Questions and verified Answers Already Passed graded
A+
What are System Independent Parameters?
Affinity
Efficacy
What is a Scatchard Plot?
plots the total amount of bound ligand divided by its free
concentration (B/[L]) against the total bound ligand, B.
What are the three types of displacement binding? (3) What is
there impact on IC50?
Competitive - As the concentration of radioligand goes up,
IC50 goes up.
Noncompetitive - IC50 does not change with increasing
radioligand
,Allosteric - IC50 goes up with increasing radioligand
Complete inhibition is not possible at high concentrations of
radioligand (saturable)
What are the first 4 criteria for Binding Experiments?
1. The binding is saturable
2. The binding is reversible and able to be displaced by other
ligands
3. There is a ligand available to determine non-specific
binding.
4. There is sufficient biological binding material to yield a
good signal-to-noise ratio but no too much so as to cause
depletion of the tracer ligand.
What are the second 4 criteria for Binding Experiments?
5. For optimum binding experiments, the following conditions
should be met: There is a high degree of specific binding and
a concomitantly low degree of non-specific binding
6. Agonist and antagonist tracer ligands are available
7. The kinetics of binding are rapid
8. The ligand used for determination of non-specific binding
has a different molecular structure from the tracer ligand.
, What are the assay groups?
1, 2, 3
What is Assay group 1?
What is an example pf how it is tested?
- Measure cell/tissue function
- Highly amplified
---Most sensitive
---Partial agonists appear as full agonists
-----Can't compare agonists
Microphysiometry - in vitro measurement of the functions and
activities of life or of living matter and of the physical and
chemical phenomena involved on a very small scale.
What is Assay group 2?
- Measure second messengers
- G proteins activate adenylate cyclase
---Measure cAMP
- Calcium flux influences many cellular processes
Questions and verified Answers Already Passed graded
A+
What are System Independent Parameters?
Affinity
Efficacy
What is a Scatchard Plot?
plots the total amount of bound ligand divided by its free
concentration (B/[L]) against the total bound ligand, B.
What are the three types of displacement binding? (3) What is
there impact on IC50?
Competitive - As the concentration of radioligand goes up,
IC50 goes up.
Noncompetitive - IC50 does not change with increasing
radioligand
,Allosteric - IC50 goes up with increasing radioligand
Complete inhibition is not possible at high concentrations of
radioligand (saturable)
What are the first 4 criteria for Binding Experiments?
1. The binding is saturable
2. The binding is reversible and able to be displaced by other
ligands
3. There is a ligand available to determine non-specific
binding.
4. There is sufficient biological binding material to yield a
good signal-to-noise ratio but no too much so as to cause
depletion of the tracer ligand.
What are the second 4 criteria for Binding Experiments?
5. For optimum binding experiments, the following conditions
should be met: There is a high degree of specific binding and
a concomitantly low degree of non-specific binding
6. Agonist and antagonist tracer ligands are available
7. The kinetics of binding are rapid
8. The ligand used for determination of non-specific binding
has a different molecular structure from the tracer ligand.
, What are the assay groups?
1, 2, 3
What is Assay group 1?
What is an example pf how it is tested?
- Measure cell/tissue function
- Highly amplified
---Most sensitive
---Partial agonists appear as full agonists
-----Can't compare agonists
Microphysiometry - in vitro measurement of the functions and
activities of life or of living matter and of the physical and
chemical phenomena involved on a very small scale.
What is Assay group 2?
- Measure second messengers
- G proteins activate adenylate cyclase
---Measure cAMP
- Calcium flux influences many cellular processes
