CO SAMP
TISSUE INCREASED METHO WAVE- REAGENT REFER
ENZYME D USED
PRINCIPLE SUBSTRATE ENZY PRODUCT LE CALCULATION
VAL
SOURCE IN
ME LENGTH USED
USED
Liver Liver LD catalyzes the
Heart disease oxidation of Working
Pyruvate Absorbance 370
Lactate Kidney Renal lactate by Serum reagent A
Kinetic Lactate NAD+ + 340nm x 132-24
Dehydrogenase Skeletal disease NAD+ to form 25uL and B
NADH 6508
muscle MI pyruvate and 1:4 ratio
RBCs Hemolysis NADH.
Alkaline
phosphatase
catalyzes in
All tissues Bone alkaline medium
esp. in cell diseases the transfer of
370
membrane, Rickets the phosphate
Working Men-1
Placenta, Osteomal group from 4- 4- AMP- Absorbance
Alkaline Kinetic Serum reagent A Gurlie
Intestinal acia’ nitrophenylphos Nitrophenyl AMP Phosphate + 405nm x
Phosphatase 20 uL and B 105
epithelium, Bone phate to 2- phosphate 4-Nitrophenol 2764
1:4 ratio
Kidney growth amino-2-
tubules pregnanc methyl-1-
y propanol
(AMP),
liberating 4-
nitrophenol
TISSUE INCREASED METHO WAVE- REAGENT REFER
ENZYME D USED
PRINCIPLE SUBSTRATE ENZY PRODUCT LE CALCULATION
VAL
SOURCE IN
ME LENGTH USED
USED
Liver Liver LD catalyzes the
Heart disease oxidation of Working
Pyruvate Absorbance 370
Lactate Kidney Renal lactate by Serum reagent A
Kinetic Lactate NAD+ + 340nm x 132-24
Dehydrogenase Skeletal disease NAD+ to form 25uL and B
NADH 6508
muscle MI pyruvate and 1:4 ratio
RBCs Hemolysis NADH.
Alkaline
phosphatase
catalyzes in
All tissues Bone alkaline medium
esp. in cell diseases the transfer of
370
membrane, Rickets the phosphate
Working Men-1
Placenta, Osteomal group from 4- 4- AMP- Absorbance
Alkaline Kinetic Serum reagent A Gurlie
Intestinal acia’ nitrophenylphos Nitrophenyl AMP Phosphate + 405nm x
Phosphatase 20 uL and B 105
epithelium, Bone phate to 2- phosphate 4-Nitrophenol 2764
1:4 ratio
Kidney growth amino-2-
tubules pregnanc methyl-1-
y propanol
(AMP),
liberating 4-
nitrophenol
