CHAPTER 10: DNA containing viruses • Electron microscopy – for epidemiologic study
but not routinely done
• EIA for gastroenteritis serotypes 40 & 41
• Serotyping by serum neutralization or
hemagglutination inhibition
Family Herpesviridae
• Common name: Herpesvirus
• Large family of viruses
• Icosahedral symmetry and surrounded by a
lipid-containing envelope
• Genome is linear, double-stranded DNA
• Latent infections may last for the life span of
the host, usually in ganglial or lymphoblastoid
cells
• Replication: nucleus
Double-Stranded DNA Viruses
Family Adenoviridae Human herpesviruses include:
• Common name: Adenovirus ➢ Herpes simplex virus types 1 and 2 – oral and
▪ Non-enveloped viruses exhibiting icosahedral genital lesions
symmetry ➢ Varicella-zoster virus – chickenpox and
▪ Genome is linear, double-stranded DNA shingles
• Replication: nucleus ➢ Epstein-Barr virus – infectious mononucleosis
▪ At least 51 types infect humans, especially in and associated with human neoplasms)
mucous membranes, and some types can ➢ Cytomegalovirus
persist in lymphoid tissue ➢ Human herpesviruses 6 and 7 (T lymphotropic)
➢ Human herpesvirus 8 (associated with Kaposi
Transmission: sarcoma)
1. Genus Simplexvirus
- Aerosols
- Fecal-oral route Virus: Herpes Simplex virus (HSV or HHV) – 1 and 2
- Fomites
- Personal contact Transmission: direct contact with infected secretions
Diseases: Disease:
• Respiratory diseases
➢ Children – serotypes 1,2,5 o Divided into 2 categories
➢ Adults – 3,4,7 ➢ Primary – first or initial infection
• Epidemic keratoconjunctivitis (highly ➢ Recurrent – reactivation of the latent
contagious) – 8, 18, 37 virus
• Infantile gastroenteritis – 40, 41 o Most infectious during early days of primary
• Pharyngoconjunctival fever (swimming pool infection
conjunctivitis) – 3, 7 o Virus infected cells are usually found in the
• Acute hemorrhagic cystitis in children – 11, 21 edge and in the base of lesions
o However the virus can be transmitted from
Diagnosis: older lesions as well as from asymptomatic
patients
• Cell culture: Human Embryonic Kidney and • HHV-1
other continuous epithelial cell lines ▪ Oropharyngeal herpes
• Fluorescent antibody ▪ Keratoconjunctivitis
• Nucleic acid test ▪ Eczema herpeticum
• EIA for gastroenteritis serotypes 40 & 41 ▪ Encephalitis
• HHV-2
▪ Genital Herpes
1|Page Chrissal Marie Caberoy
but not routinely done
• EIA for gastroenteritis serotypes 40 & 41
• Serotyping by serum neutralization or
hemagglutination inhibition
Family Herpesviridae
• Common name: Herpesvirus
• Large family of viruses
• Icosahedral symmetry and surrounded by a
lipid-containing envelope
• Genome is linear, double-stranded DNA
• Latent infections may last for the life span of
the host, usually in ganglial or lymphoblastoid
cells
• Replication: nucleus
Double-Stranded DNA Viruses
Family Adenoviridae Human herpesviruses include:
• Common name: Adenovirus ➢ Herpes simplex virus types 1 and 2 – oral and
▪ Non-enveloped viruses exhibiting icosahedral genital lesions
symmetry ➢ Varicella-zoster virus – chickenpox and
▪ Genome is linear, double-stranded DNA shingles
• Replication: nucleus ➢ Epstein-Barr virus – infectious mononucleosis
▪ At least 51 types infect humans, especially in and associated with human neoplasms)
mucous membranes, and some types can ➢ Cytomegalovirus
persist in lymphoid tissue ➢ Human herpesviruses 6 and 7 (T lymphotropic)
➢ Human herpesvirus 8 (associated with Kaposi
Transmission: sarcoma)
1. Genus Simplexvirus
- Aerosols
- Fecal-oral route Virus: Herpes Simplex virus (HSV or HHV) – 1 and 2
- Fomites
- Personal contact Transmission: direct contact with infected secretions
Diseases: Disease:
• Respiratory diseases
➢ Children – serotypes 1,2,5 o Divided into 2 categories
➢ Adults – 3,4,7 ➢ Primary – first or initial infection
• Epidemic keratoconjunctivitis (highly ➢ Recurrent – reactivation of the latent
contagious) – 8, 18, 37 virus
• Infantile gastroenteritis – 40, 41 o Most infectious during early days of primary
• Pharyngoconjunctival fever (swimming pool infection
conjunctivitis) – 3, 7 o Virus infected cells are usually found in the
• Acute hemorrhagic cystitis in children – 11, 21 edge and in the base of lesions
o However the virus can be transmitted from
Diagnosis: older lesions as well as from asymptomatic
patients
• Cell culture: Human Embryonic Kidney and • HHV-1
other continuous epithelial cell lines ▪ Oropharyngeal herpes
• Fluorescent antibody ▪ Keratoconjunctivitis
• Nucleic acid test ▪ Eczema herpeticum
• EIA for gastroenteritis serotypes 40 & 41 ▪ Encephalitis
• HHV-2
▪ Genital Herpes
1|Page Chrissal Marie Caberoy
