END
ANALYTE NORMAL
TYPE OF NAME OF CHROMOGEN PRODUCT/
TO BE SPECIMEN REAGENT USED REFERENCE
METHOD METHOD USED POSITIVE
TESTED VALUE
COLOR
Alkaline
Whole copper Folin-Wu Alkaline copper tartrate Phosphomolybdic
Glucose Blue 80-120 mg/dL
Blood reduction Method Phosphomolybdic acid solution acid solution
method
Whole blood
Alkaline Alkaline copper tartrate
Blue 65-95 mg/dL
copper Somogyi- Arsenomlybdate
Glucose Serum (molybdenum
reduction Nelson method Arsenomlybdate
blue) Serum:
method
80-110 mg/dL
Phosphate buffer
Phenol
Serum/Plasma:
4-aminoantipyrine
Enzymatic 60-100 mg/dL
Glucose oxidase
Serum, Colorimetric Glucose 4- Pink (3.30-
Glucose Peroxidase
Plasma, CSF Test, oxidase aminoantipyrine (Quinoneimine) 5.60mmol/L)
Trinder children, adult
Wavelength if at 500nm
Temperature is 16-25C or 37C
Reagent A:
Tris, 2-oxoglutarate, Urease, Glutamate Serum/Plasma
dehydrogenase, Ethylene glycol, Sodium Urea:
Serum Urease- azide Glutamate 12.8-
Urea Plasma Kinetic Glutamase Reagent B: ---- + 42.8mg/dL
Fresh Urine dehydrogenase NADH NAD+ (2.14 - 7.14
Wavelength if at 340nm mmol/L)
Temperature is 370C
Serum and
Urine
plasma:
Reagent A: Men
Men 0.9
Sodium Hydroxide 14 – 26
1.3mg/dL
Serum mg/ kg/24hr
(80-115
Creatinine Plasma ____ Jaffe Reagent B: Dark yellow
umol/L)
Urine Picric Acid Women
Women 0.6-
11-20
1.1 mg/dL
Wavelength is at 500nm mg/kg/24hr
(53-97 umol/L)
ANALYTE NORMAL
TYPE OF NAME OF CHROMOGEN PRODUCT/
TO BE SPECIMEN REAGENT USED REFERENCE
METHOD METHOD USED POSITIVE
TESTED VALUE
COLOR
Alkaline
Whole copper Folin-Wu Alkaline copper tartrate Phosphomolybdic
Glucose Blue 80-120 mg/dL
Blood reduction Method Phosphomolybdic acid solution acid solution
method
Whole blood
Alkaline Alkaline copper tartrate
Blue 65-95 mg/dL
copper Somogyi- Arsenomlybdate
Glucose Serum (molybdenum
reduction Nelson method Arsenomlybdate
blue) Serum:
method
80-110 mg/dL
Phosphate buffer
Phenol
Serum/Plasma:
4-aminoantipyrine
Enzymatic 60-100 mg/dL
Glucose oxidase
Serum, Colorimetric Glucose 4- Pink (3.30-
Glucose Peroxidase
Plasma, CSF Test, oxidase aminoantipyrine (Quinoneimine) 5.60mmol/L)
Trinder children, adult
Wavelength if at 500nm
Temperature is 16-25C or 37C
Reagent A:
Tris, 2-oxoglutarate, Urease, Glutamate Serum/Plasma
dehydrogenase, Ethylene glycol, Sodium Urea:
Serum Urease- azide Glutamate 12.8-
Urea Plasma Kinetic Glutamase Reagent B: ---- + 42.8mg/dL
Fresh Urine dehydrogenase NADH NAD+ (2.14 - 7.14
Wavelength if at 340nm mmol/L)
Temperature is 370C
Serum and
Urine
plasma:
Reagent A: Men
Men 0.9
Sodium Hydroxide 14 – 26
1.3mg/dL
Serum mg/ kg/24hr
(80-115
Creatinine Plasma ____ Jaffe Reagent B: Dark yellow
umol/L)
Urine Picric Acid Women
Women 0.6-
11-20
1.1 mg/dL
Wavelength is at 500nm mg/kg/24hr
(53-97 umol/L)
