Biochemistry 1 Lab Review for Final Exam Pt. 1
Latest Update 2025-2026 120 Questions and
100% Verified Correct Answers Guaranteed A+
(SDS) Sodium Dodecyl Sulfate - CORRECT ANSWER: __________ is used to coat the
protein with a negative charge and to aid in the denaturing process.
(SDS) Sodium Dodecyl Sulfate - CORRECT ANSWER: What was added to the sample
buffer used in the electrophoresis chamber in the Purification and size determination of
gfp and bfp experiment?
(SDS) Sodium Dodecyl Sulfate in the buffer - CORRECT ANSWER: The sudsing
(bubbles) in the gel buffer are due to the _________ in the Purification and size
determination of gfp and bfp experiment.
0 to 1 - CORRECT ANSWER: Rf values vary from _____.
0.05 ml - CORRECT ANSWER: Which of the following volumes of water CANNOT be
transferred with a 100-1000 micro-liter range pipette?
A. 0.05 ml
B. 0.10 ml
C. 0.75 ml
1 micro-liter - CORRECT ANSWER: How many micro-liter is 0.001 ml?
1 micro-liter=0.001 ml
800 micro-liter (0.001 ml/1 micro-liter)
= 0.8 ml= 0.8g H2O - CORRECT ANSWER: Give the mass in grams of 800 micro-liters
of pure water.
, 1 ml - 5 ml - CORRECT ANSWER: What amounts of liquids do serological pipettes
transfer?
1. Choose the correct pipette based on the amount of liquid you will need.
2. Set the pipette to the amount needed and lock the pipette if lock is provided.
3. Put the correct tip on the pipette.
4. Push the plunger to the first stop.
5. Put the tip of the pipette just below the surface of the liquid.
6. Let go of the plunger SLOWLY.
7. To dispense the liquid put the pipette on the side of the tube with the tube turn and
the pipette VERTICAL.
8. Push the plunger to the second stop and keep it held until the pipette is out of the
tube.
9. Push the tip remover.
10. Dispose of the used tip. - CORRECT ANSWER: List in proper order, the steps
required to correctly pipette.
1. Glycine (Gly)
2. Alanine (Ala)
3. Proline (Pro)
4. Phenylalanine (Phe)
5. Methionine (Met)
6. Histidine (His)
7. Leucine (Leu)
8. Valine (Val) - CORRECT ANSWER: List the amino acids with abbreviations used in
the Amino Acid chromatography exercise.
1. Lab rules, lab safety and Pipetting
Latest Update 2025-2026 120 Questions and
100% Verified Correct Answers Guaranteed A+
(SDS) Sodium Dodecyl Sulfate - CORRECT ANSWER: __________ is used to coat the
protein with a negative charge and to aid in the denaturing process.
(SDS) Sodium Dodecyl Sulfate - CORRECT ANSWER: What was added to the sample
buffer used in the electrophoresis chamber in the Purification and size determination of
gfp and bfp experiment?
(SDS) Sodium Dodecyl Sulfate in the buffer - CORRECT ANSWER: The sudsing
(bubbles) in the gel buffer are due to the _________ in the Purification and size
determination of gfp and bfp experiment.
0 to 1 - CORRECT ANSWER: Rf values vary from _____.
0.05 ml - CORRECT ANSWER: Which of the following volumes of water CANNOT be
transferred with a 100-1000 micro-liter range pipette?
A. 0.05 ml
B. 0.10 ml
C. 0.75 ml
1 micro-liter - CORRECT ANSWER: How many micro-liter is 0.001 ml?
1 micro-liter=0.001 ml
800 micro-liter (0.001 ml/1 micro-liter)
= 0.8 ml= 0.8g H2O - CORRECT ANSWER: Give the mass in grams of 800 micro-liters
of pure water.
, 1 ml - 5 ml - CORRECT ANSWER: What amounts of liquids do serological pipettes
transfer?
1. Choose the correct pipette based on the amount of liquid you will need.
2. Set the pipette to the amount needed and lock the pipette if lock is provided.
3. Put the correct tip on the pipette.
4. Push the plunger to the first stop.
5. Put the tip of the pipette just below the surface of the liquid.
6. Let go of the plunger SLOWLY.
7. To dispense the liquid put the pipette on the side of the tube with the tube turn and
the pipette VERTICAL.
8. Push the plunger to the second stop and keep it held until the pipette is out of the
tube.
9. Push the tip remover.
10. Dispose of the used tip. - CORRECT ANSWER: List in proper order, the steps
required to correctly pipette.
1. Glycine (Gly)
2. Alanine (Ala)
3. Proline (Pro)
4. Phenylalanine (Phe)
5. Methionine (Met)
6. Histidine (His)
7. Leucine (Leu)
8. Valine (Val) - CORRECT ANSWER: List the amino acids with abbreviations used in
the Amino Acid chromatography exercise.
1. Lab rules, lab safety and Pipetting
