QBM FINAL EXAM QUESTIONS AND
CORRECT ANSWERS
The process of taking a gene from one source and joining it with the DNA from another
source than inserting it into a bacteria to further manipulate it. - CORRECT
ANSWER✅✅Recombinant DNA Technology
What is the central dogma? - CORRECT ANSWER✅✅the TRANSCRIPTION from DNA
to mRNA and the TRANSLATION from mRNA to Protein
What type of structure consist of alpha-helice and beta sheets? - CORRECT
ANSWER✅✅secondary structures
When multiple polypeptide chains interact together they form a? - CORRECT
ANSWER✅✅quaternary structure
How are proteins synthesized? - CORRECT ANSWER✅✅By the ribosome from the N to
the C terminus
When vexing DNA under microscope what would it look like? - CORRECT
ANSWER✅✅Molecular surface (solvent accessible surface)
Which organization holds employers responsible for informing their workers of potential
hazards? - CORRECT ANSWER✅✅OSHA
What does an Material Safety Data Sheet state? - CORRECT ANSWER✅✅Chemical's
physicals and chemicals properties, health hazards, association hazards, primary route of
entry, exposure limits, toxic, radioactive, or flammable substances.
This organization funded by the government regulates labs working with dangerous
organism? - CORRECT ANSWER✅✅CDC
Where in UCF can we find a BSL-4 Lab - CORRECT ANSWER✅✅No where, most UCF
labs are BSL-2 with some research labs been BSL-3
A lab working with organisms that are a moderate potential hazard, proper equipment should
be worn, and waste should be discarded appropriately should obtain what Biosafety Level? -
CORRECT ANSWER✅✅BSL-2
What laboratories should have a BSL-3 level? - CORRECT ANSWER✅✅those working
with pathogenic organisms that can cause serious diseases
If a professor in UCF starts researching an organism that not much is known about it exert
that it can cause severe even fatal diseases to which no vaccine has been created. His lab
should have which BSL level? - CORRECT ANSWER✅✅BSL-4
, If while working in the QBM lab at UCF should accidentally spill some radioactive substance
who should be notified to clean the spill? - CORRECT ANSWER✅✅Department of
Environmental Health and Safety
What are consider first barrier methods in lab? - CORRECT ANSWER✅✅Goggles, Lab
coats, gloves.
What is the preferred type of gloves for lab? - CORRECT ANSWER✅✅Nitrile
T/F a needle should be recapped before placed in the sharps container to minimize the
chances of sticking by the individual emptying the container. - CORRECT
ANSWER✅✅False, the needles should not be recapped as that increases the chances of
you sticking your self.
What must you do to a bacteria before discarding it? - CORRECT ANSWER✅✅Killed
with 10% bleacher autoclaved.
Which government branch oversees workplace safety? - CORRECT ANSWER✅✅OSHA
What is often required to fully sterilize glassware,pipette tips, and cell media? - CORRECT
ANSWER✅✅Autoclave
If you were to transfer 1L from a container to a beaker, what should you use to measure it? -
CORRECT ANSWER✅✅Graduate cylinder
Plastic pipettes should be used when measuring things between ____ and ____? - CORRECT
ANSWER✅✅1 to 25ml
T/F when aspiring a liquid with a micropipette, you should push the plunger down all the way
to ensure tag the proper amount of liquid is take up. - CORRECT ANSWER✅✅False, it
should be to the first stop.
A pipette would give you the most accurate volume when inserted 1.5cm into the media. -
CORRECT ANSWER✅✅False, it should be 1cm
what type of centrifuge should be used when purifying a protein sample? - CORRECT
ANSWER✅✅fixed analog rotors which are excellent for pelleting a sample.
Which type of centrifuge can be used for sample analysis or virus purification? - CORRECT
ANSWER✅✅Swinging bucket rotor
When DNA replicates and the strands unwind and are used to make hybrid daughter strands
on both old and new DNA? - CORRECT ANSWER✅✅Semiconservative
When DNA is randomly distributed in the daughter strands. - CORRECT
ANSWER✅✅dispersive
CORRECT ANSWERS
The process of taking a gene from one source and joining it with the DNA from another
source than inserting it into a bacteria to further manipulate it. - CORRECT
ANSWER✅✅Recombinant DNA Technology
What is the central dogma? - CORRECT ANSWER✅✅the TRANSCRIPTION from DNA
to mRNA and the TRANSLATION from mRNA to Protein
What type of structure consist of alpha-helice and beta sheets? - CORRECT
ANSWER✅✅secondary structures
When multiple polypeptide chains interact together they form a? - CORRECT
ANSWER✅✅quaternary structure
How are proteins synthesized? - CORRECT ANSWER✅✅By the ribosome from the N to
the C terminus
When vexing DNA under microscope what would it look like? - CORRECT
ANSWER✅✅Molecular surface (solvent accessible surface)
Which organization holds employers responsible for informing their workers of potential
hazards? - CORRECT ANSWER✅✅OSHA
What does an Material Safety Data Sheet state? - CORRECT ANSWER✅✅Chemical's
physicals and chemicals properties, health hazards, association hazards, primary route of
entry, exposure limits, toxic, radioactive, or flammable substances.
This organization funded by the government regulates labs working with dangerous
organism? - CORRECT ANSWER✅✅CDC
Where in UCF can we find a BSL-4 Lab - CORRECT ANSWER✅✅No where, most UCF
labs are BSL-2 with some research labs been BSL-3
A lab working with organisms that are a moderate potential hazard, proper equipment should
be worn, and waste should be discarded appropriately should obtain what Biosafety Level? -
CORRECT ANSWER✅✅BSL-2
What laboratories should have a BSL-3 level? - CORRECT ANSWER✅✅those working
with pathogenic organisms that can cause serious diseases
If a professor in UCF starts researching an organism that not much is known about it exert
that it can cause severe even fatal diseases to which no vaccine has been created. His lab
should have which BSL level? - CORRECT ANSWER✅✅BSL-4
, If while working in the QBM lab at UCF should accidentally spill some radioactive substance
who should be notified to clean the spill? - CORRECT ANSWER✅✅Department of
Environmental Health and Safety
What are consider first barrier methods in lab? - CORRECT ANSWER✅✅Goggles, Lab
coats, gloves.
What is the preferred type of gloves for lab? - CORRECT ANSWER✅✅Nitrile
T/F a needle should be recapped before placed in the sharps container to minimize the
chances of sticking by the individual emptying the container. - CORRECT
ANSWER✅✅False, the needles should not be recapped as that increases the chances of
you sticking your self.
What must you do to a bacteria before discarding it? - CORRECT ANSWER✅✅Killed
with 10% bleacher autoclaved.
Which government branch oversees workplace safety? - CORRECT ANSWER✅✅OSHA
What is often required to fully sterilize glassware,pipette tips, and cell media? - CORRECT
ANSWER✅✅Autoclave
If you were to transfer 1L from a container to a beaker, what should you use to measure it? -
CORRECT ANSWER✅✅Graduate cylinder
Plastic pipettes should be used when measuring things between ____ and ____? - CORRECT
ANSWER✅✅1 to 25ml
T/F when aspiring a liquid with a micropipette, you should push the plunger down all the way
to ensure tag the proper amount of liquid is take up. - CORRECT ANSWER✅✅False, it
should be to the first stop.
A pipette would give you the most accurate volume when inserted 1.5cm into the media. -
CORRECT ANSWER✅✅False, it should be 1cm
what type of centrifuge should be used when purifying a protein sample? - CORRECT
ANSWER✅✅fixed analog rotors which are excellent for pelleting a sample.
Which type of centrifuge can be used for sample analysis or virus purification? - CORRECT
ANSWER✅✅Swinging bucket rotor
When DNA replicates and the strands unwind and are used to make hybrid daughter strands
on both old and new DNA? - CORRECT ANSWER✅✅Semiconservative
When DNA is randomly distributed in the daughter strands. - CORRECT
ANSWER✅✅dispersive
