BIOD 171 LAB 6 NOTE
Title: GE06 Enzymatic Assays
Objective: To profile bacterial populations based on key enzymatic
reactions and determine growth/metabolic characteristics
Procedure:
-Oxidase test
1. Using a sterile loop, pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine the test area for colour change within 20 seconds
-Catalase test
1. Using a sterile loop, carefully pick the colony from the plate
2. Smear colony directly onto the glass slide
3. Add two drops of hydrogen peroxide to each smear
-Coagulase test
1. Using a sterile loop, carefully pick a colony from the plate
2. Add the colony to the tube of rabbit plasma and incubate
overnight at 37C
3. (next day) examine samples for precipitants
-Lipase test
1. Using a sterile loop of unknown culture onto spirit blue plates
2. Streak colony across the plate, incubate overnight at 37C
Title: GE06 Enzymatic Assays
Objective: To profile bacterial populations based on key enzymatic
reactions and determine growth/metabolic characteristics
Procedure:
-Oxidase test
1. Using a sterile loop, pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine the test area for colour change within 20 seconds
-Catalase test
1. Using a sterile loop, carefully pick the colony from the plate
2. Smear colony directly onto the glass slide
3. Add two drops of hydrogen peroxide to each smear
-Coagulase test
1. Using a sterile loop, carefully pick a colony from the plate
2. Add the colony to the tube of rabbit plasma and incubate
overnight at 37C
3. (next day) examine samples for precipitants
-Lipase test
1. Using a sterile loop of unknown culture onto spirit blue plates
2. Streak colony across the plate, incubate overnight at 37C
