BIOD 171 LAB 7 NOTE
Title: GE07 Secondary Assays for Bacterial Characterization
Objective: To profile bacterial population based on additional
biochemical approaches to better determine growth/metabolic
characteristics
Procedure:
Indole Test: Test #1
1. pick an isolated colony from BAP
2. using a sterile loop. Smear directly onto the reaction area of
the slide
3. Examine the test area for colour change within 20 seconds
Indole Test: Test #2
1. Using a sterile loop, pick an isolated colony from BAP
2. Inoculate bacteria in media broth
3. Grow at 37C overnight
4. Add ~5 drops of Kovac’s reagent to culture
TSI Test
1. Use a sterile loop to inoculate the slant from the liquid culture
2. Press loop into agar slant
3. While removing the loop, streak slanted side of the agar with
the loop
4. Grow at 37C overnight
5. (next day) Examine samples
Title: GE07 Secondary Assays for Bacterial Characterization
Objective: To profile bacterial population based on additional
biochemical approaches to better determine growth/metabolic
characteristics
Procedure:
Indole Test: Test #1
1. pick an isolated colony from BAP
2. using a sterile loop. Smear directly onto the reaction area of
the slide
3. Examine the test area for colour change within 20 seconds
Indole Test: Test #2
1. Using a sterile loop, pick an isolated colony from BAP
2. Inoculate bacteria in media broth
3. Grow at 37C overnight
4. Add ~5 drops of Kovac’s reagent to culture
TSI Test
1. Use a sterile loop to inoculate the slant from the liquid culture
2. Press loop into agar slant
3. While removing the loop, streak slanted side of the agar with
the loop
4. Grow at 37C overnight
5. (next day) Examine samples
