Biochemistry 1 Lab Review for Final Exam Pt.
1 Questions and Answers (100% Correct
Answers) Already Graded A+
Quiz: List this years Biochemistry 1 Lab Titles.⼀Answer: 1. Lab rules, lab
safety and Pipetting
2. Amino Acid Chromatography
3. Purification and size determination of gfp and bfp
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4. Protein Assays
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5. Enzyme Kinetics
6. Affinity Chromatography
Quiz: We will be using ______ displacement pipettes in this lab.⼀
Answer: Air
Quiz: If you are late to class, you will not be allowed to take the quiz.⼀
Answer: True
Quiz: You may take pictures of notes on the board.⼀Answer: False
Quiz: Give the mass in grams of 800 micro-liters of pure water.⼀
Answer: 1 micro-liter=0.001 ml
800 micro-liter (0.001 ml/1 micro-liter)
= 0.8 ml= 0.8g H2O
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Quiz: Which of the following volumes of water CANNOT be transferred
with a 100-1000 micro-liter range pipette?
A. 0.05 ml
B. 0.10 ml
C. 0.75 ml⼀Answer: 0.05 ml
Quiz: Explain why the pipette tip should be placed just below the surface
of the liquid when drawing liquids up into the
tip.⼀Answer: Because if the pipette is placed deep, some of the liquid of
the tube might stay on the outside of the pipette tip and therefore be
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transferred into the receiving container which can make your results
incorrect.
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Quiz: Diagram the INCORRECT tip positions of dispensing liquid from
a pipette.⼀Answer: See figures.
Quiz: List in proper order, the steps required to correctly pipette.⼀
Answer: 1. Choose the correct pipette based on the amount of liquid you
will need.
2. Set the pipette to the amount needed and lock the pipette if lock is
provided.
3. Put the correct tip on the pipette.
4. Push the plunger to the first stop.
5. Put the tip of the pipette just below the surface of the liquid.
6. Let go of the plunger SLOWLY.
7. To dispense the liquid put the pipette on the side of the tube with the tube
turn and the pipette VERTICAL.
8. Push the plunger to the second stop and keep it held until the pipette is
out of the tube.
9. Push the tip remover.
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10. Dispose of the used tip.
Quiz: At what angle should the receiving tube be when transferring
liquids with a pipette?⼀Answer: 45 degree angle
Quiz: What amounts of liquids do micro pipettes transfer?⼀Answer:
Small amounts < 1 ml
Quiz: What amounts of liquids do serological pipettes transfer?⼀Answer:
1 ml - 5 ml
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Quiz: What is the difference between Accuracy and Precision?⼀Answer:
Accuracy is how close to the target and Precision is how close the values are
to each other.
Quiz: What is the equation of density?⼀Answer: D=M/V
Density= Mass / Volume
Quiz: What is the density of water at 25 C?⼀Answer: 1.0 g/ml
Quiz: How many micro-liter is 0.001 ml?⼀Answer: 1 micro-liter
Quiz: In grams, what is the weight of 100 micro-liter of water at room
temperature?⼀Answer: 100 micro-liter( 1ml/1000 micro-liter)
=0.1 ml = 0.1 g H2O
Because 1ml H2O = 1g H2O