ASCP CHEMISTRY PROTEINS & ENZYMES
CERTIFICATION MASTERY: METABOLIC
PATHWAY ANALYSIS & CLINICAL
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,What specimen preparation is commonly
used to perform the alkaline
phosphatase isoenzyme determination?
A. Serum is divided into 2 aliquots, one
is frozen and the other is refrigerated
B. Serum is divided into aliquots, one is
heated at 56C and the other is unheated
C. No preparation is necessary since the
assay used EDTA plasma
D. Protein-free filtrate is prepared first -
answer: B. Serum is divided into
aliquots, one is heated at 56C and the
other is unheated
The heat activation method of ALP
isoenzyme separation involves heating
an aliquot of the serum sample at 56C
for 10 minutes. AN untreated aliquot of
the sample along with heated one are
assayed for ALP activity.
,The most specific enzyme test for acute
pancreatitis is:
A. Acid phosphatase
B. Trypsin
C. Amylase
D. Lipase - answer: D. Lipase
There is an increase in the serum levels
of amylase and lipase in acute
pancreatitis. However, the elevated lever
of lipase persists longer than amylase.
Elevated levels of lipase and amylase are
seen in other intra-abdominal
conditions, but the frequency of
elevations is less with lapse than
amylase.
Which of the following enzymes are used
in the diagnosis of acute pancreatitis?
A. Amylase (AMS)
B. Aspartate aminotransferase (AST)
C. Gamma-glutamyl Transferase (GGT)
, D. Lactate dehydrogenase (LD) - answer:
A. Amylase (AMS)
Amylase and lipase are hydrolases
involved in the breakdown of starch and
glycogen, and lipid metabolism,
respectively. Both enzymes are primarily
located in the pancreas. Disorders of the
pancreas are characterized by elevated
levels of the enzymes.
Which of the following enzymes
catalyzes the conversion of starch to
glucose and maltose?
A. Malate dehydrogenase (MD)
B. Amylase (AMS)
C. Creatine kinase (CK)
D. Isocitric dehydrogenase (ICD) -
answer: B. Amylase (AMS)
In the amyloclastic, saccharogenic and
chromogenic methods for easurement of
amylase, the substrate, starch is
converted to glucose and maltose.
CERTIFICATION MASTERY: METABOLIC
PATHWAY ANALYSIS & CLINICAL
DISORDER DIAGNOSTICS | QUICK RECALL
SYSTEM | LATEST UPDATE GUARANTEED
SUCCESS
\
,What specimen preparation is commonly
used to perform the alkaline
phosphatase isoenzyme determination?
A. Serum is divided into 2 aliquots, one
is frozen and the other is refrigerated
B. Serum is divided into aliquots, one is
heated at 56C and the other is unheated
C. No preparation is necessary since the
assay used EDTA plasma
D. Protein-free filtrate is prepared first -
answer: B. Serum is divided into
aliquots, one is heated at 56C and the
other is unheated
The heat activation method of ALP
isoenzyme separation involves heating
an aliquot of the serum sample at 56C
for 10 minutes. AN untreated aliquot of
the sample along with heated one are
assayed for ALP activity.
,The most specific enzyme test for acute
pancreatitis is:
A. Acid phosphatase
B. Trypsin
C. Amylase
D. Lipase - answer: D. Lipase
There is an increase in the serum levels
of amylase and lipase in acute
pancreatitis. However, the elevated lever
of lipase persists longer than amylase.
Elevated levels of lipase and amylase are
seen in other intra-abdominal
conditions, but the frequency of
elevations is less with lapse than
amylase.
Which of the following enzymes are used
in the diagnosis of acute pancreatitis?
A. Amylase (AMS)
B. Aspartate aminotransferase (AST)
C. Gamma-glutamyl Transferase (GGT)
, D. Lactate dehydrogenase (LD) - answer:
A. Amylase (AMS)
Amylase and lipase are hydrolases
involved in the breakdown of starch and
glycogen, and lipid metabolism,
respectively. Both enzymes are primarily
located in the pancreas. Disorders of the
pancreas are characterized by elevated
levels of the enzymes.
Which of the following enzymes
catalyzes the conversion of starch to
glucose and maltose?
A. Malate dehydrogenase (MD)
B. Amylase (AMS)
C. Creatine kinase (CK)
D. Isocitric dehydrogenase (ICD) -
answer: B. Amylase (AMS)
In the amyloclastic, saccharogenic and
chromogenic methods for easurement of
amylase, the substrate, starch is
converted to glucose and maltose.
