Laboratory Manual
,Provide New Learning Pathways
to Understand the Why Behind
the Science
C ASE ST U DY
HAND WASHING AND ASEPTIC TECHNIQUE: A CASE STUDY
You are presented with an eosin–methylene blue Due to cost and time restrictions, your lab
(EMB) agar plate that has bacterial colonies with is limited regarding how many assays can be
a slight greenish, metallic sheen. Your laboratory performed. Using a series of biochemical tests to
manager explains the background for the culture confirm or refute the analysis of the processing
you are observing on the plate: An unknown con- plant, you will need to determine whether the
taminate was found in a meat processing machine, bacteria is an enteric and then whether it is an
and the in-house pathogen control office performed E. coli isolate.
a swab and a streak on an EMB plate. After incu-
bation and observation of the weak reaction, the
Questions to Consider:
manufacturers concluded that the contaminate was
not E. coli and that no further tests were required. 1. Does the lack of a strong reaction on the EMB
Upper management decided that to protect the plate refute the determination that the isolated
company from potential lawsuits, they would hire bacteria are an E. coli culture?
the laboratory you work for to ensure that their 2. Which series of assays would best be used to
laboratory technicians concluded correctly. prove/disprove the E. coli determination?
NEW! Clinical Case Studies now within each section of the lab
manual bring career relevance to the lab experiments. These open-ended
cases can be used to fuel class discussion and group work about the topics
covered in lab.
NEW! Further F URT HE R RE A DIN G
Reading Sections
Refer to the section on antimicrobial compounds
help students know
in your textbook for further information on the
where to look in their compounds that have an effect on bacterial cells.
textbook if they need In your textbook’s index, search under “Chemo-
more background therapy,” “Antibiotics,” and “Analog.”
information to
understand the
science behind the
experiment.
,Connect Lecture and Lab with
Mastering Microbiology
MicroLab Practical
Activities assess
students’ observation
skills and give them
extra practice to
analyze important lab
tests, procedures, and
results.
Prepare for lab with pre-lab quizzes for each of the 72 experiments in
Microbiology: A Laboratory Manual Twelfth Edition, and then follow up to
measure comprehension with post-lab quizzes in Mastering Microbiology™.
, And Come to Class Better
Prepared for Lab
Videos and Coaching
Activities help instructors
and students get the most out of
lab time. Students can practice
their lab skills virtually reviewing
proper lab techniques with real-
world applications. Live action
video combined with molecular
animation with assessment and
feedback coach students how to
interpret and analyze different
lab results.
Lab Technique Videos give students an opportunity to see techniques performed correctly
and quiz themselves on lab procedures both before and after lab time, improving confidence and
proficiency. Assign as pre-lab quizzes in Mastering Microbiology and include coaching and feedback
on a wide range of lab techniques.
,Provide New Learning Pathways
to Understand the Why Behind
the Science
C ASE ST U DY
HAND WASHING AND ASEPTIC TECHNIQUE: A CASE STUDY
You are presented with an eosin–methylene blue Due to cost and time restrictions, your lab
(EMB) agar plate that has bacterial colonies with is limited regarding how many assays can be
a slight greenish, metallic sheen. Your laboratory performed. Using a series of biochemical tests to
manager explains the background for the culture confirm or refute the analysis of the processing
you are observing on the plate: An unknown con- plant, you will need to determine whether the
taminate was found in a meat processing machine, bacteria is an enteric and then whether it is an
and the in-house pathogen control office performed E. coli isolate.
a swab and a streak on an EMB plate. After incu-
bation and observation of the weak reaction, the
Questions to Consider:
manufacturers concluded that the contaminate was
not E. coli and that no further tests were required. 1. Does the lack of a strong reaction on the EMB
Upper management decided that to protect the plate refute the determination that the isolated
company from potential lawsuits, they would hire bacteria are an E. coli culture?
the laboratory you work for to ensure that their 2. Which series of assays would best be used to
laboratory technicians concluded correctly. prove/disprove the E. coli determination?
NEW! Clinical Case Studies now within each section of the lab
manual bring career relevance to the lab experiments. These open-ended
cases can be used to fuel class discussion and group work about the topics
covered in lab.
NEW! Further F URT HE R RE A DIN G
Reading Sections
Refer to the section on antimicrobial compounds
help students know
in your textbook for further information on the
where to look in their compounds that have an effect on bacterial cells.
textbook if they need In your textbook’s index, search under “Chemo-
more background therapy,” “Antibiotics,” and “Analog.”
information to
understand the
science behind the
experiment.
,Connect Lecture and Lab with
Mastering Microbiology
MicroLab Practical
Activities assess
students’ observation
skills and give them
extra practice to
analyze important lab
tests, procedures, and
results.
Prepare for lab with pre-lab quizzes for each of the 72 experiments in
Microbiology: A Laboratory Manual Twelfth Edition, and then follow up to
measure comprehension with post-lab quizzes in Mastering Microbiology™.
, And Come to Class Better
Prepared for Lab
Videos and Coaching
Activities help instructors
and students get the most out of
lab time. Students can practice
their lab skills virtually reviewing
proper lab techniques with real-
world applications. Live action
video combined with molecular
animation with assessment and
feedback coach students how to
interpret and analyze different
lab results.
Lab Technique Videos give students an opportunity to see techniques performed correctly
and quiz themselves on lab procedures both before and after lab time, improving confidence and
proficiency. Assign as pre-lab quizzes in Mastering Microbiology and include coaching and feedback
on a wide range of lab techniques.
