Chemistry Notebook
Name:
Date: 2/24/25
Experiment #: 5
Title: Buffers
Purpose: The purpose of this lab is to learn about buffers and their ability resistant to pH
change. We will examine how a buffer solution is composed as well as the relationship between
pH and enzyme activity.
Procedure:
Experiment #1:
1. Two beakers were gathered and one beaker was filled up with water and the other
beaker with the buffer solution.
2. Using a pH meter, each electrode from the pH meter was connected to each beaker.
3. The starting pH is around 7 in the pH meter.
4. A scoop of sodium acetate was added to the water beaker and another scoop of
sodium acetate was added to the beaker with the buffer solution.
5. With the electrodes in each beaker, the pH reading from the pH meter was recorded.
6. The beakers were removed from the pH meter and the electrodes were rinsed with water
7. With two new beakers, water was added to one beaker and the same buffer solution as
step one was added to the other beaker.
8. Both beakers were connected to the electrodes connecting to the pH meter.
9. The starting pH is around 7 in the pH meter.
10. 5 drops of HCl were added to the beaker with water.
11. 5 drops of HCl were added to the beaker with the buffer solution.
12. The solutions in each beaker were gently stirred with a rod.
13. The pH levels from the pH meter were recorded.
14. A few more drops of HCl were added to each beaker and the the pH levels were
recorded again.
Experiment #2: Using Equal Concentrations
Name:
Date: 2/24/25
Experiment #: 5
Title: Buffers
Purpose: The purpose of this lab is to learn about buffers and their ability resistant to pH
change. We will examine how a buffer solution is composed as well as the relationship between
pH and enzyme activity.
Procedure:
Experiment #1:
1. Two beakers were gathered and one beaker was filled up with water and the other
beaker with the buffer solution.
2. Using a pH meter, each electrode from the pH meter was connected to each beaker.
3. The starting pH is around 7 in the pH meter.
4. A scoop of sodium acetate was added to the water beaker and another scoop of
sodium acetate was added to the beaker with the buffer solution.
5. With the electrodes in each beaker, the pH reading from the pH meter was recorded.
6. The beakers were removed from the pH meter and the electrodes were rinsed with water
7. With two new beakers, water was added to one beaker and the same buffer solution as
step one was added to the other beaker.
8. Both beakers were connected to the electrodes connecting to the pH meter.
9. The starting pH is around 7 in the pH meter.
10. 5 drops of HCl were added to the beaker with water.
11. 5 drops of HCl were added to the beaker with the buffer solution.
12. The solutions in each beaker were gently stirred with a rod.
13. The pH levels from the pH meter were recorded.
14. A few more drops of HCl were added to each beaker and the the pH levels were
recorded again.
Experiment #2: Using Equal Concentrations
