BLD 430 FINAL EXAM QUESTIONS AND
CORRECT ANSWERS
proper handling of specimen - answer - no pre analytical error
- proper label and ID
- chain of custody
- avoid hemolysis and cross contamination
reject specimen if - answer - not labeled or identified
- hemolysis
- cross contamination
- pre analytical error
- collected in incorrect tube
- incorrect specimen type
DNA Storage: blood, bone marrow, fluids - answer - less than or equal to 1 day, 23C
- 3 days at 4C
- WBC, greater than or equal to 1 year 020C or -70C
DNA Storage: tissue - answer - 23C
- less than or equal to 1 day, 4C
- greater than or equal to 2 weeks, -20C
- greater than or equal to 2 years, -70C
DNA Storage: isolated DNA - answer - less than or equal to 26 weeks, 2-25C
- 1-3 years, 4C
- less than or equal to 7 years, -20C, -70C
RNA Storage: blood, bone marrow, fluids - answer - less than 2 hours, 23C or 4C
- 5 days, 23C
- 7 days, 4C in denaturant
, - 1-2 weeks, -70C in denaturant
RNA Storage: tissue - answer - less than 2 hours, 4C
- snap frozen, -70C, greater than or equal to 2 years
- nitrogen vapor, -140 to -150C, greater than or equal to 2 years
RNA Storage: isolated RNA - answer - 2-25C
- less than 30 days, -20C in DEPC treated water
- less than 30 days, -70C in DEPC treated water
- greater than 6 months, -70C in ethanol
organization that established standards used by MLS when developing, implementing,
and performing molecular based tests - answer Clinical and Laboratory Standards
Institute
Sensitivity Calculation - answer (TP)/(TP+FN) X 100
Specificity Calculation - answer (TN)/(TN+FP) X 100
Accuracy Calculation - answer (TN+TP)/(TN+TP+FN+FP)
Positive control - answer run in a sample to make sure you don't get a false negative
- to make sure that all of the reagents are working
negative control - answer run a sample you know is negative (has all reagents but no
sample) to make sure you don't get a false positive
sensitivity control - answer defines the lower limit of detection of a molecular target
amplification control - answer avoid false negative results, target always amplified
How often check refrigerator temp - answer every day
- can't vary by more than 2C
organization that provides oversight and governs the use of radioactive material in the
lab - answer NFPA
What needs to be documented - answer - test results
- assay conditions
- reagent lot numbers
- quality and quantity of the isolated DNA/RNA
CORRECT ANSWERS
proper handling of specimen - answer - no pre analytical error
- proper label and ID
- chain of custody
- avoid hemolysis and cross contamination
reject specimen if - answer - not labeled or identified
- hemolysis
- cross contamination
- pre analytical error
- collected in incorrect tube
- incorrect specimen type
DNA Storage: blood, bone marrow, fluids - answer - less than or equal to 1 day, 23C
- 3 days at 4C
- WBC, greater than or equal to 1 year 020C or -70C
DNA Storage: tissue - answer - 23C
- less than or equal to 1 day, 4C
- greater than or equal to 2 weeks, -20C
- greater than or equal to 2 years, -70C
DNA Storage: isolated DNA - answer - less than or equal to 26 weeks, 2-25C
- 1-3 years, 4C
- less than or equal to 7 years, -20C, -70C
RNA Storage: blood, bone marrow, fluids - answer - less than 2 hours, 23C or 4C
- 5 days, 23C
- 7 days, 4C in denaturant
, - 1-2 weeks, -70C in denaturant
RNA Storage: tissue - answer - less than 2 hours, 4C
- snap frozen, -70C, greater than or equal to 2 years
- nitrogen vapor, -140 to -150C, greater than or equal to 2 years
RNA Storage: isolated RNA - answer - 2-25C
- less than 30 days, -20C in DEPC treated water
- less than 30 days, -70C in DEPC treated water
- greater than 6 months, -70C in ethanol
organization that established standards used by MLS when developing, implementing,
and performing molecular based tests - answer Clinical and Laboratory Standards
Institute
Sensitivity Calculation - answer (TP)/(TP+FN) X 100
Specificity Calculation - answer (TN)/(TN+FP) X 100
Accuracy Calculation - answer (TN+TP)/(TN+TP+FN+FP)
Positive control - answer run in a sample to make sure you don't get a false negative
- to make sure that all of the reagents are working
negative control - answer run a sample you know is negative (has all reagents but no
sample) to make sure you don't get a false positive
sensitivity control - answer defines the lower limit of detection of a molecular target
amplification control - answer avoid false negative results, target always amplified
How often check refrigerator temp - answer every day
- can't vary by more than 2C
organization that provides oversight and governs the use of radioactive material in the
lab - answer NFPA
What needs to be documented - answer - test results
- assay conditions
- reagent lot numbers
- quality and quantity of the isolated DNA/RNA
