lOMoAR cPSD| 17224140
Medical Laboratory Science Comprehensive Exams
Ultimate Compilation
Medical Technology
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
CLINICAL CHEMISTRY
CC – 2nd take
Coupled enzymatic rgt except
T4 in RID Radiolabeled T4 binding with Anti T4
Lithium in blood serum AES/flame
Renal threshold When there’s an excess the substance is no
longer reabsorb so it goes out and appear in
the urine
GGT Liver
Metabolite increase in liver dse Ammonia
Starch to simple saccharide Amylase
Enzyme catalyze from starch to glucose and Amylase
maltose
Cortisol diurnal characteristic 8-10 peak morning 11-3 evening low
Middle value Median
Tungsten blue Uric acid in Caraway method. Also in
measuring urinary proteins under the
Folin-Ciocalteau method.
Most methods of tAG determination Glycerol
measure :
Reyes syndrome Ammonia
Colorimetric bilirubin method
Uncompemsated acidosis Low pH increase Carbonic acid
Bilirubin bile canaliculi Dubin Johnson
Protein not usually present in blood Alpha 1 anti-globulin
Tube for glycated Hb EDTA – normally used for Hemoglobin
measurements
Conversion of urea nitrogen to urea Multiply by 2.14
VLDL Major carrier of ENDOGENOUS
triglycerides.
SGOT Reitman Frankel
Bitter almond Cyanide
Does not need fasting HbA1C
Glucose level of DM >126ug/dl
Chloride mercuric nitrite producing blue Schales schales
color
Zero order Excess substrate / no excess enzyme
Etched pipette Blow out pipette
Ascorbic acid inhibits w/c glucose pathway Oxidase-reduction
monochromator Selects the band of the light that passes to
the cuvette
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
AAS calcium
No included enzymatic test in in
determining glucose
Interference in bilirubin test @ 640nm Anything that is infecting the integrity of
the serum. (Hemolysis ganyan – dapat clear
yung serum na yellow color)
Dilution 1:5 4ml H2O
Scattered light Nephelometry
precision The lower the Standard Deviation the
higher the precision
Icteric index Bilirubin since Icterus is increase in
Bilirubin
Monitor quality control between labs Youden plot
(inter-lab)
Bilirubin excretion deficiency Dubin Johnson
Chelates calcium EDTA
Cholesterol reference method Abell-kendall (mataba si Abell)
% glucose in PB sa CSF 60%
Major fraction organic iodine in circulation Thyroxine T4? (Confirm Clin. Chem
module)
drug GC/MS
monochromator Disposes polychromatic light into its
separate wavelengths
Turbidity in serum chylomicrons
CLINICAL CHEMISTRY
Creatine Kinase(CK) for Acute Myocardial CK-MB and CK MM – Reference is
Infarction Bishop’s p 272. Confirm with CC2 module
Blue color (urea, nitrogen, ammonia, uric Uric Acid – (Tungsten blue)
acid)
Cleaning glassware EXCEPT: Cr2H2O/H2SO4
Cytoplasmic protein transport?? Ligandin and Z protein
Hexokinase NADPH
Specific for liver ALT
Inversely related to CHD Apo A & HDL
Incorrect use of pipette Random error
Gibson Cooke test? Which is true? Refer to pg 105 CC2 (Testing for Cystic
Fibrosis, sweat test. Stimulate with
pilocarpine.)
Reproducibility Precision
+2SD 95.5%
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
Steps for cholesterol Saponification, extraction ,precipitation,
coulorometric (SEPC)
Benzoylecgonine Cocaine,
100% T = Optical density % 0.000
Primary minerals in bone Calcium & phosphate
Respiratory acidosis Excess carbonic acid leading to
decreased pH. To compensate for this
kidneys will increase amount of
bicarbonate.
Substrate of prostatic ACP Thymolphalein monophosphate…
(something like that)
Several test in CC required the px to for cholesterol
fasting (Fasting blood sugar, Chole, Trigly,
inorg.phosphate?)
Conjugated bilirubin characteristics Water and alcohol soluble, renal
excretion, direct conjugated,
“Red man syndrome” Vancomycin
YUNG SA CHARTS AOTA
Do not move in pre-beta... Chylomicrons
Split in albumin Bisalbuminemia
Albumin Ref range 3.5-5.0 g/dl
Heroin synthesis from morphine
Quantitation of TAG glycerol
Test for neuromuscular tetany Low calcium / hypocalcemia
Ref. Range of plasma chole. 200 mg/dl
Csf multiple sclerosis characteristics Polyclonal banding
Test for decrease T4 TSH
Sweat chloride is for Cystic fibrosis (Gibson Cooke Method)
Random errors Dirty glasswares, wrong pipets, voltage,
fluctation, sampling errors
Hypophyseal structure receive signals Hypothylamoneurophyseal tract?
Catalyzes the conjugation of bilirubin Glucoronly transferase
ISE are associated EXCEPT: None of the above?
Transport protein of bilirubin in blood albumin
Ion increase in hemolysis K because it is major intracellular anion.
Cellulose acetate principle Size of molecule
Lipoprotein increased
Enzyme that converts starch to glucose and Amylase
maltose
Serotonin source Tryptophan
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
Medical Laboratory Science Comprehensive Exams
Ultimate Compilation
Medical Technology
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
CLINICAL CHEMISTRY
CC – 2nd take
Coupled enzymatic rgt except
T4 in RID Radiolabeled T4 binding with Anti T4
Lithium in blood serum AES/flame
Renal threshold When there’s an excess the substance is no
longer reabsorb so it goes out and appear in
the urine
GGT Liver
Metabolite increase in liver dse Ammonia
Starch to simple saccharide Amylase
Enzyme catalyze from starch to glucose and Amylase
maltose
Cortisol diurnal characteristic 8-10 peak morning 11-3 evening low
Middle value Median
Tungsten blue Uric acid in Caraway method. Also in
measuring urinary proteins under the
Folin-Ciocalteau method.
Most methods of tAG determination Glycerol
measure :
Reyes syndrome Ammonia
Colorimetric bilirubin method
Uncompemsated acidosis Low pH increase Carbonic acid
Bilirubin bile canaliculi Dubin Johnson
Protein not usually present in blood Alpha 1 anti-globulin
Tube for glycated Hb EDTA – normally used for Hemoglobin
measurements
Conversion of urea nitrogen to urea Multiply by 2.14
VLDL Major carrier of ENDOGENOUS
triglycerides.
SGOT Reitman Frankel
Bitter almond Cyanide
Does not need fasting HbA1C
Glucose level of DM >126ug/dl
Chloride mercuric nitrite producing blue Schales schales
color
Zero order Excess substrate / no excess enzyme
Etched pipette Blow out pipette
Ascorbic acid inhibits w/c glucose pathway Oxidase-reduction
monochromator Selects the band of the light that passes to
the cuvette
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
AAS calcium
No included enzymatic test in in
determining glucose
Interference in bilirubin test @ 640nm Anything that is infecting the integrity of
the serum. (Hemolysis ganyan – dapat clear
yung serum na yellow color)
Dilution 1:5 4ml H2O
Scattered light Nephelometry
precision The lower the Standard Deviation the
higher the precision
Icteric index Bilirubin since Icterus is increase in
Bilirubin
Monitor quality control between labs Youden plot
(inter-lab)
Bilirubin excretion deficiency Dubin Johnson
Chelates calcium EDTA
Cholesterol reference method Abell-kendall (mataba si Abell)
% glucose in PB sa CSF 60%
Major fraction organic iodine in circulation Thyroxine T4? (Confirm Clin. Chem
module)
drug GC/MS
monochromator Disposes polychromatic light into its
separate wavelengths
Turbidity in serum chylomicrons
CLINICAL CHEMISTRY
Creatine Kinase(CK) for Acute Myocardial CK-MB and CK MM – Reference is
Infarction Bishop’s p 272. Confirm with CC2 module
Blue color (urea, nitrogen, ammonia, uric Uric Acid – (Tungsten blue)
acid)
Cleaning glassware EXCEPT: Cr2H2O/H2SO4
Cytoplasmic protein transport?? Ligandin and Z protein
Hexokinase NADPH
Specific for liver ALT
Inversely related to CHD Apo A & HDL
Incorrect use of pipette Random error
Gibson Cooke test? Which is true? Refer to pg 105 CC2 (Testing for Cystic
Fibrosis, sweat test. Stimulate with
pilocarpine.)
Reproducibility Precision
+2SD 95.5%
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
, lOMoAR cPSD| 17224140
Steps for cholesterol Saponification, extraction ,precipitation,
coulorometric (SEPC)
Benzoylecgonine Cocaine,
100% T = Optical density % 0.000
Primary minerals in bone Calcium & phosphate
Respiratory acidosis Excess carbonic acid leading to
decreased pH. To compensate for this
kidneys will increase amount of
bicarbonate.
Substrate of prostatic ACP Thymolphalein monophosphate…
(something like that)
Several test in CC required the px to for cholesterol
fasting (Fasting blood sugar, Chole, Trigly,
inorg.phosphate?)
Conjugated bilirubin characteristics Water and alcohol soluble, renal
excretion, direct conjugated,
“Red man syndrome” Vancomycin
YUNG SA CHARTS AOTA
Do not move in pre-beta... Chylomicrons
Split in albumin Bisalbuminemia
Albumin Ref range 3.5-5.0 g/dl
Heroin synthesis from morphine
Quantitation of TAG glycerol
Test for neuromuscular tetany Low calcium / hypocalcemia
Ref. Range of plasma chole. 200 mg/dl
Csf multiple sclerosis characteristics Polyclonal banding
Test for decrease T4 TSH
Sweat chloride is for Cystic fibrosis (Gibson Cooke Method)
Random errors Dirty glasswares, wrong pipets, voltage,
fluctation, sampling errors
Hypophyseal structure receive signals Hypothylamoneurophyseal tract?
Catalyzes the conjugation of bilirubin Glucoronly transferase
ISE are associated EXCEPT: None of the above?
Transport protein of bilirubin in blood albumin
Ion increase in hemolysis K because it is major intracellular anion.
Cellulose acetate principle Size of molecule
Lipoprotein increased
Enzyme that converts starch to glucose and Amylase
maltose
Serotonin source Tryptophan
Medical Laboratory Science Comprehensive Exams Ultimate Compilation
