Chapter 15 (Translation) with
100% correct answers ( graded
A+)
machinery responsible for translation - answer mRNA
tRNA
aminoacyl-tRNA synthetases
ribosome
each ORF specifies - answer a single protein
ends of an ORF are distinct from - answer the ends of mRNA
mRNA is a template for - answer translation
tRNAs provide the physical interface between - answer amino
acids
aminoacyl-tRNA synthetases couple amino acids to - answer
specific tRNAs that recognize the appropriate codons
the ribosome coordinates - answer the correct recognition of the
mRNA by each tRNA and catalyzes peptide bond formation
,stop codons define - answer the end of the ORF
shine-dalgarno sequence is also called - answer ribosomal binding
site (RBS)
in translational coupling, mutations affect - answer 2 or more
proteins
eukaryotic ribosomes can recruit ribosomes using their - answer
5' cap
kozak sequence features - answer a purine 3 bases upstream and
a guanine immediately downstream
features that promote active translation - answer high
complementarity and proper spacing
poly A tail enhances the level of translation of the mRNA by -
answer enhancing the recuitment of key translation initiation
factors
each tRNA recognizes - answer a particular codon
all tRNAs end at the - answer 3' terminus, with sequence 5'-CCA-3'
, 3' end of tRNA is the site that - answer is attached to the next
amino acid
tRNAs have unique structures, including - answer pseudouridine
and dihydrouridine
hypoxanthine, thymine, and methylguanine
cells lacking in the modified bases found in tRNA have shown -
answer reduced rates of growth
acceptor stem - answer site of attachment of the amino acid
psi-U stem - answer characteristic of unusual bases
D stem - answer has dihydrouridines in this group
anticodon stem - answer contains the anticodon
anticodon is always bracketed on the 3' end by a - answer purine
anticodon is always bracketed on the 5' end by a - answer uracil
the tertiary structure of tRNA is - answer L shaped
the secondary structure of tRNA is - answer cloverleaf shaped