13 (Mechanisms of Transcription)
with 100% correct answers
( graded A+)
RNAP is an enzyme that - answer catalyzes RNA synthesis
RNAP can initiate transcription - answer de novo
multiple RNAP molecules can transcribe the same gene - answer
at the same time, each following closely behind each other
transcription, in comparison to DNA replication, is - answer less
accurate
transcription only copies - answer certain parts of the genome
replication copies - answer the entire genome
# of RNAP in bacteria - answer 1 RNAP
# of RNAP in eukayotes - answer 3 RNAP
the RNAP most focused on in eukayotes - answer RNAP II
,why is RNAP II most focused on for study - answer it transcribes
the most genes
RNAP I & III transcribe - answer RNA-encoding genes
RNAP I transcribes - answer the large rRNA precursor gene
RNAP III transcribes - answer tRNA genes, some small nuclear
RNA genes, and the 5S rRNA gene
RNAP IV & V are found - answer only in plants
RNAP IV & V transcribe - answer small interfering RNAs involved in
transcription silencing
the bacterial RNAP core enzyme alone is capable of - answer
synthesizing RNA
contents of the core enzyme of bacterial RNAP - answer 2 alpha,
one beta, one beta prime, and two omega (w) subunits
the beta and beta prime subunits in bacterial RNAP core enzymes
resemble in eukaryotes the - answer RPB1 and RPB2 subunits
, the alpha subunit in bacterial RNAP core enzymes resemble in
eukaryotes the - answer RPB3 and RPB11 subunits
the omega (w) subunit in bacterial RNAP core enzymes resemble
in eukaryotes the - answer RPB6 subunit
active center cleft is the - answer active site of transcription in
RNAP
transcription occurs in the direction of - answer 5'-3'
different promoters transcribe - answer different genes
closed complex - answer both strands of DNA are together, RNAP
bound to one face of the helix
open complex - answer RNAP has pulled the strands apart and the
strands are not held together
initiation shifts into elongation after - answer RNAP has
synthesized a short stretch of RNA (~10BP)
transcription bubble - answer an open complex of DNA to allow
transcription, about 13 BP long
holoenzyme - answer initiation (sigma) factor + core enzyme