1
BOIC 385 EXAM 4 LATEST UPDATES -2025/2026- ACTUAL
QUESTIONS WITH VERIFIED ANSWERS ALREADY GRADED A+
GUARANTEED SUCCESS
Which of the following is correct concerning self-splicing introns?
None of these answers are correct
What effect is likely from the alteration of the sequence at the 5' end of the RNA
component of the U1 snRNP from 5'AUACYYACCU3" to 5'AUACYYGCCU3'? (Note
that Y in the sequence stands for the modified base "psi") Choose the ONE best
choice from the list below.
The 5' splicing junction that will be recognized by this altered spliceosome will be
5'AGGCAGGU3' because the nucleotide sequence of the base pairing portion of
U1 snRNP has changed.
What is a Holliday Junction? Choose the one BEST answer below.
The location in a DNA recombination intermediate where DNA strands from
different DNA molecules are crossed over, which links the two DNA molecules
together.
Select the THREE statements below that describe BOTH DNA replication and RNA
transcription in E. coli.
The direction of polymerization is 5' to 3'
A specific region of the DNA is recognized and bound by the polymerase.
The direction of enzyme movement on the template strand is 3' to 5'
Match the name of the DNA repair mechanism with the correct description.
, 2
mismatch-Functions with DNA replication to remove incorrect nucleotides from
the nascent strand.
base excision repair-Requires an endonuclease to remove an abasic nucleotide
before DNA Pol I can replace a portion of the strand.
nucleotide excision repair-Is needed to repair chemical damage in DNA effecting
more than one nucleotide, such as cyclobutene pyrimidine dimers.
direct repair-Repairs nucleotide bases without requiring DNA ligase.
What accounts for the high fidelity of DNA polymerization? Choose the ONE best
answer.
Active site geometry ensures that only correct sized nucleotide base pairs are
formed, and 3' to 5' proofreading removes incorrect nucleotides.
What is the function of the MutS-MutL-MutH protein complex?
None of these answers are correct.
If 15N DNA replicated using conservative replication in 14N media, the outcome
would be that new DNA has
one high density and one low density strand
In the figure show below of an E. coli replication fork, which protein is responsible
for relieving torsional stress?
Gyrase
Helicase is responsible for unwinding the DNA duplex for replication. This action
leads to torsional strain upstream of the replication fork, which would inhibit
forward movement of the fork. Gyrase, which is a type II topoisomerase, relieves
this strain.
Based on the figure below, cleavage followed by rejoining at which sites would
lead to no recombination or loss of genetic information?
1 and 3
, 3
AZT is such a good HIV drug because it
prevents new DNA from being replicated.
Francis Crick hypothesized that the Genetic Code must require 3 nucleotides in
the mRNA to specify each amino acid based on a simple calculation. What was the
logic behind this calculation? Choose the ONE best answer.
Based on the number of known amino acids and the number of known nucleotide
bases.
What explains the observation that a single eukaryotic protein coding gene can
give rise to multiple different proteins, i.e., why are there only ~25,000 human
genes in the genome, but ~150,000 different proteins in the human "proteome?"
Choose the TWO best correct answers.
mRNA transcripts from the same gene can be differentially spliced to
include/exclude exons.
Genes can contain more than one polyadenylation site, which alters the 3' of the
mRNA transcript and the inclusion/exclusion of exons.
The processes of DNA replication and RNA transcription in E. coli are similar in
some respects and different in others.
Select the THREE statements below that describe BOTH DNA replication and RNA
transcription in E. coli.
The direction of enzyme movement on the template strand is 3' to 5'
The direction of enzyme movement on the template strand is 3' to 5'
, 4
A specific region of the DNA is recognized and bound by the polymerase.
Why does RNA contain uracil and DNA contain thymine? Choose the ONE best
answer to this question.
None of these statements are correct.
Nirenberg and colleagues determined a portion of the genetic code using filter
binding assays that included ribosomes, trinucleotides and tRNA molecules
charged with radioactively-labeled amino acids. Choose the answer below that
correctly answers the following three questions in order:
1) If the trinucleotide sequence is CAG, which amino acid will be bound to the
filter paper?
2) If the wobble position of the trinucleotide sequence is changed to a U, which
amino acid will be bound to the filter paper?
3) True or False: Two distinct tRNA molecules with different anticodon sequences
MUST be used to recognize the codons AGA and AGG.
1) Gln, 2) His, 3) False
The Shine-Dalgarno sequence of mRNA base pairs with the __________ rRNA
within the prokaryote __________ ribosomal subunit.
16S; 30S
The initiation, elongation, and termination of eukaryotic transcription is
controlled by the
extent of phosphorylation and dephosphorylation of the RNA polymerase CTD.
BOIC 385 EXAM 4 LATEST UPDATES -2025/2026- ACTUAL
QUESTIONS WITH VERIFIED ANSWERS ALREADY GRADED A+
GUARANTEED SUCCESS
Which of the following is correct concerning self-splicing introns?
None of these answers are correct
What effect is likely from the alteration of the sequence at the 5' end of the RNA
component of the U1 snRNP from 5'AUACYYACCU3" to 5'AUACYYGCCU3'? (Note
that Y in the sequence stands for the modified base "psi") Choose the ONE best
choice from the list below.
The 5' splicing junction that will be recognized by this altered spliceosome will be
5'AGGCAGGU3' because the nucleotide sequence of the base pairing portion of
U1 snRNP has changed.
What is a Holliday Junction? Choose the one BEST answer below.
The location in a DNA recombination intermediate where DNA strands from
different DNA molecules are crossed over, which links the two DNA molecules
together.
Select the THREE statements below that describe BOTH DNA replication and RNA
transcription in E. coli.
The direction of polymerization is 5' to 3'
A specific region of the DNA is recognized and bound by the polymerase.
The direction of enzyme movement on the template strand is 3' to 5'
Match the name of the DNA repair mechanism with the correct description.
, 2
mismatch-Functions with DNA replication to remove incorrect nucleotides from
the nascent strand.
base excision repair-Requires an endonuclease to remove an abasic nucleotide
before DNA Pol I can replace a portion of the strand.
nucleotide excision repair-Is needed to repair chemical damage in DNA effecting
more than one nucleotide, such as cyclobutene pyrimidine dimers.
direct repair-Repairs nucleotide bases without requiring DNA ligase.
What accounts for the high fidelity of DNA polymerization? Choose the ONE best
answer.
Active site geometry ensures that only correct sized nucleotide base pairs are
formed, and 3' to 5' proofreading removes incorrect nucleotides.
What is the function of the MutS-MutL-MutH protein complex?
None of these answers are correct.
If 15N DNA replicated using conservative replication in 14N media, the outcome
would be that new DNA has
one high density and one low density strand
In the figure show below of an E. coli replication fork, which protein is responsible
for relieving torsional stress?
Gyrase
Helicase is responsible for unwinding the DNA duplex for replication. This action
leads to torsional strain upstream of the replication fork, which would inhibit
forward movement of the fork. Gyrase, which is a type II topoisomerase, relieves
this strain.
Based on the figure below, cleavage followed by rejoining at which sites would
lead to no recombination or loss of genetic information?
1 and 3
, 3
AZT is such a good HIV drug because it
prevents new DNA from being replicated.
Francis Crick hypothesized that the Genetic Code must require 3 nucleotides in
the mRNA to specify each amino acid based on a simple calculation. What was the
logic behind this calculation? Choose the ONE best answer.
Based on the number of known amino acids and the number of known nucleotide
bases.
What explains the observation that a single eukaryotic protein coding gene can
give rise to multiple different proteins, i.e., why are there only ~25,000 human
genes in the genome, but ~150,000 different proteins in the human "proteome?"
Choose the TWO best correct answers.
mRNA transcripts from the same gene can be differentially spliced to
include/exclude exons.
Genes can contain more than one polyadenylation site, which alters the 3' of the
mRNA transcript and the inclusion/exclusion of exons.
The processes of DNA replication and RNA transcription in E. coli are similar in
some respects and different in others.
Select the THREE statements below that describe BOTH DNA replication and RNA
transcription in E. coli.
The direction of enzyme movement on the template strand is 3' to 5'
The direction of enzyme movement on the template strand is 3' to 5'
, 4
A specific region of the DNA is recognized and bound by the polymerase.
Why does RNA contain uracil and DNA contain thymine? Choose the ONE best
answer to this question.
None of these statements are correct.
Nirenberg and colleagues determined a portion of the genetic code using filter
binding assays that included ribosomes, trinucleotides and tRNA molecules
charged with radioactively-labeled amino acids. Choose the answer below that
correctly answers the following three questions in order:
1) If the trinucleotide sequence is CAG, which amino acid will be bound to the
filter paper?
2) If the wobble position of the trinucleotide sequence is changed to a U, which
amino acid will be bound to the filter paper?
3) True or False: Two distinct tRNA molecules with different anticodon sequences
MUST be used to recognize the codons AGA and AGG.
1) Gln, 2) His, 3) False
The Shine-Dalgarno sequence of mRNA base pairs with the __________ rRNA
within the prokaryote __________ ribosomal subunit.
16S; 30S
The initiation, elongation, and termination of eukaryotic transcription is
controlled by the
extent of phosphorylation and dephosphorylation of the RNA polymerase CTD.
