FIS 314 Final questions with verified
answers to pass
T/F: Magnification with the light microscope takes place in two steps; the first is the creation of
a real, magnified, and inverted image within the body tube of the microscope. - correct answer
✔✔true
T/F: If you plan to vacuum sweep and tape lift the trunk of an automobile, best practice dictates
that you vacuum sweep first. - correct answer ✔✔false
T/F: As magnification increases, numerical aperture increases, angular aperture increases, and
resolution increases. - correct answer ✔✔true
If you view a diffraction test grating with a spacing of 0.15um, can you resolve the grating's
details using an objective/aperture diaphragm angular aperture of 100 degrees and blus (400
nm) light? - correct answer ✔✔no
A clean room is under (positive/negative) pressure. - correct answer ✔✔positive
An objective is stamped with the following information: 0.17, infinity symbol, 60X, and 0.85.
What is the angular aperture of this objective (dry)? - correct answer ✔✔116 degrees
(NA = n sin (AA/2)
An objective is stamped with the following information: 0.7, infinity symbol, 60X, and 0.85.
What is the numerical aperture of this objective (dry)? - correct answer ✔✔0.85
, If you view a diffraction grating with blue light (400 nm) using an objective/aperture diaphragm
combination with a numerical aperture of 0.65, what is the radius of the Airy disc in um? -
correct answer ✔✔0.375 um
r = (0.61 x 400 nm)/0.65 = 375 nm
T/F: The aperture diaphragm is part of the image forming rays, and it is conjugate with the light
source and the specimen plane. - correct answer ✔✔false
T/F: As numerical aperture increases, the radius of the Airy disc increases. - correct answer
✔✔false
T/F: As magnification increases, working distance increases. - correct answer ✔✔false
T/F: As magnification decreases, depth of field decreases. - correct answer ✔✔false
T/F: As the radius of the Airy disc increases, resolution increases. - correct answer ✔✔false
T/F: Refraction is greater (higher) at shorter wavelengths, but diffraction is greater (higher) at
longer wavelengths. - correct answer ✔✔true
T/F: As temperature increases, density decreases, speed of light increases, and refractive index
decreases. - correct answer ✔✔true
What is the standard room temperature C that Cargille liquids are labeled with? - correct
answer ✔✔25C
You receive a Cargille standard refractive index liquid with a refractive index of 1.568 at room
temperature. You are using it in a room at 20C. What is its refractive index at this lower
temperature if its temperature coefficient is 0.005/deg C? - correct answer ✔✔1.593
answers to pass
T/F: Magnification with the light microscope takes place in two steps; the first is the creation of
a real, magnified, and inverted image within the body tube of the microscope. - correct answer
✔✔true
T/F: If you plan to vacuum sweep and tape lift the trunk of an automobile, best practice dictates
that you vacuum sweep first. - correct answer ✔✔false
T/F: As magnification increases, numerical aperture increases, angular aperture increases, and
resolution increases. - correct answer ✔✔true
If you view a diffraction test grating with a spacing of 0.15um, can you resolve the grating's
details using an objective/aperture diaphragm angular aperture of 100 degrees and blus (400
nm) light? - correct answer ✔✔no
A clean room is under (positive/negative) pressure. - correct answer ✔✔positive
An objective is stamped with the following information: 0.17, infinity symbol, 60X, and 0.85.
What is the angular aperture of this objective (dry)? - correct answer ✔✔116 degrees
(NA = n sin (AA/2)
An objective is stamped with the following information: 0.7, infinity symbol, 60X, and 0.85.
What is the numerical aperture of this objective (dry)? - correct answer ✔✔0.85
, If you view a diffraction grating with blue light (400 nm) using an objective/aperture diaphragm
combination with a numerical aperture of 0.65, what is the radius of the Airy disc in um? -
correct answer ✔✔0.375 um
r = (0.61 x 400 nm)/0.65 = 375 nm
T/F: The aperture diaphragm is part of the image forming rays, and it is conjugate with the light
source and the specimen plane. - correct answer ✔✔false
T/F: As numerical aperture increases, the radius of the Airy disc increases. - correct answer
✔✔false
T/F: As magnification increases, working distance increases. - correct answer ✔✔false
T/F: As magnification decreases, depth of field decreases. - correct answer ✔✔false
T/F: As the radius of the Airy disc increases, resolution increases. - correct answer ✔✔false
T/F: Refraction is greater (higher) at shorter wavelengths, but diffraction is greater (higher) at
longer wavelengths. - correct answer ✔✔true
T/F: As temperature increases, density decreases, speed of light increases, and refractive index
decreases. - correct answer ✔✔true
What is the standard room temperature C that Cargille liquids are labeled with? - correct
answer ✔✔25C
You receive a Cargille standard refractive index liquid with a refractive index of 1.568 at room
temperature. You are using it in a room at 20C. What is its refractive index at this lower
temperature if its temperature coefficient is 0.005/deg C? - correct answer ✔✔1.593
