Bio 2104
BIO 2104 - MICRO LAB PRACTICAL
EXAM
Gram Positive
Identify the gram stain
Gram Negative
Identify the gram stain
Gram Positive
Identify the gram stain
Gram Negative
Identify the gram stain
Gram Positive
Bio 2104
, Bio 2104
Which gram stain identifies peptidoglycan?
Gram Negative
Which gram stain identifies a cell without a peptidoglycan and a thin cell membrane or
wall?
Ensures that the cells don't rupture from abrupt change in temperature during heat
fixation
When gram staining it is important to allow the smear to air dry and then heat fix it.
Why?
crystal violet
After heat fixation the first step of gram staining is to dip the smear in
___________________.
gram's iodine
After dipping a slide in crystal violet the next step is to dip the slide in
_______________.
rinse with alcohol/ethanol
After dipping the slide in gram's iodine the next step is to....
safranin
What is the final stain used when making a gram stain?
endospore stain
What test is used to distinguish endospores?
avoids the build up of condensation that could disrupt colony formation
why is it important to flip your plate before incubation?
98.6 F
what temperature is typically used for incubation of human pathogens?
genus and species
when preparing for incubation it is important to label the specimen with __________ and
___________.
Bio 2104
BIO 2104 - MICRO LAB PRACTICAL
EXAM
Gram Positive
Identify the gram stain
Gram Negative
Identify the gram stain
Gram Positive
Identify the gram stain
Gram Negative
Identify the gram stain
Gram Positive
Bio 2104
, Bio 2104
Which gram stain identifies peptidoglycan?
Gram Negative
Which gram stain identifies a cell without a peptidoglycan and a thin cell membrane or
wall?
Ensures that the cells don't rupture from abrupt change in temperature during heat
fixation
When gram staining it is important to allow the smear to air dry and then heat fix it.
Why?
crystal violet
After heat fixation the first step of gram staining is to dip the smear in
___________________.
gram's iodine
After dipping a slide in crystal violet the next step is to dip the slide in
_______________.
rinse with alcohol/ethanol
After dipping the slide in gram's iodine the next step is to....
safranin
What is the final stain used when making a gram stain?
endospore stain
What test is used to distinguish endospores?
avoids the build up of condensation that could disrupt colony formation
why is it important to flip your plate before incubation?
98.6 F
what temperature is typically used for incubation of human pathogens?
genus and species
when preparing for incubation it is important to label the specimen with __________ and
___________.
Bio 2104
