Summary ANIMAL CARE AND USE INVESTIGATOR AND STAFF HANDBOOK LABORATORY ANIMAL PROGRAM AAALAC Accredited SOUTHERN ILLINOIS UNIVERSITY CARBONDALE

ANIMAL CARE AND USE
INVESTIGATOR AND STAFF HANDBOOK




LABORATORY ANIMAL PROGRAM
AAALAC Accredited

SOUTHERN ILLINOIS UNIVERSITY CARBONDALE

http://iacuc.siu.edu/
Life Science III, Room 1062
Mailcode 6506
Phone (618) 536-2346

, CONTENTS
Page

ORGANIZATIONAL CHART …………………………………………………… 5

INTRODUCTION ...................................................................................................... 6

I. ANIMAL CARE AND USE PROTOCOL SUBMISSION AND REVIEW ....... 6
A. Protocol Preparation ………………………………….................................. 6
B. Protocol Reviews ............................................................................................. 7
1. Annual Protocol Review ........................................................................... 7
2. Triennial Protocol Review ....................................................................... 7
C. Electronic Protocol Submission Instructions .....…………………........ ..... 7
D. Changes to Approved Protocols ..... ………………………………............. 9

II. ROLES AND RESPONSIBILITIES OF PRINCIPAL INVESTIGATORS
AND RESEARCH STAFF ..................................................................................... 9
A. Roles …………………………………………………………......................... 9
B. Responsibilities ………………………………………………….................. 9
C. Research Team ..……………………………………………….................... 10
1. Occupational Health Program .....………………………………........... 10
2. Laboratory Animal Training Program ..... …………………………… 11

III. INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC) .... 11
A. Composition …………………………………………………….................... 11
B. Roles and Responsibilities………………………………………….............. 12
C. IACUC Policies ............................................................................................... 13
1. Humane Endpoints ………………………………………….................. 13
2. Animal Analgesia ...……………………………………………............. 15
3. Physical Restraint ...……………………………………………............. 16
4. Animal Surgery ………………………………………………................ 17

IV. THE LABORATORY ANIMAL PROGRAM ................................................. 20
A. Laboratory Animal Program Facilities and Procedures ………………… 20
1. Facilities…………………………………………………............................ 20
a. Animal Rooms ............................................................................... 20
b. Traffic Flow ……………………………………………………… 20
c. Documentation .............................................................................. 20
d. Husbandry ...................................................................................... 21
2. Procedures and Policies …………………………………………........... 21
a. New Investigator/Staff Check List ………………………... 21
b. Personnel Access ……………………………………................. 21


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, c. Animal Procurement .…………………………………….......... 22
d. Animal Transfers .……………………………………………….. 23
e. Technical Assistance …………………………………............... 23
f. Breeding ………………………………………………................ 23
B. Vivarium Charges ……………… .……………………………………........ 23
C. Animal Health Care ………………………………………………............. 23
1. Observation .……………………………………………….................... 24
2. Dead Animals ……………………………………………...................... 24
3. Health Surveillance ……………………………………………............. 24

V. OCCUPATIONAL HEALTH AND SAFETY ................................................. 24
A. Medical Health Form ………………………………………………........... 24
B. Reporting Injuries or Illnesses ..………………………………………....... 24
C. Personal Hygiene…………………………………………………................ 25
D. Animal Bites and Scratches ..…………………………………………....... 25
E. Handling of Sharps .……………………………………………. ............... 26
F. Allergies to Animals ……………………………………………................... 26
G. Zoonosis……………………………………………………………............... 27
1. Hantavirus ................................................................................................ 27
2. Lymphocytic Choriomeningitis Virus ....................................................... 28
3. Tetanus ...................................................................................................... 28
4. Rat-bite Fever .......................................................................................... 29
5. Leptospirosis ............................................................................................ 29
6. Plague ....................................................................................................... 29
7. Ringworm ................................................................................................. 29

APPENDIX A. REGULATIONS AND GUIDELINES ......................................... 31
Introduction ................................................................................................... 31
Animal Welfare Act ...................................................................................... 31
Public Health Service on Humane Care and Use of Laboratory Animals 32
U.S. Government Principles for the Utilization and Care of Vertebrate
Animals Used in Testing, Research, and Training ....................... 32
Association for Assessment and Accreditation of Laboratory Animal
Care International (AAALAC) ........................................................ 33

APPENDIX B. ANIMAL IDENTIFICATION ........................................................ 35
Cage Cards ...................................................................................................... 35
Animal Identification Methods .................................................................... 35

APPENDIX C. ANIMAL HANDLING AND RESTRAINT ................................. 36
General Information ....................................................................................... 36
Hand Restraint ................................................................................................ 36


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, APPENDIX D. RODENT BIOLOGY.......................................................................... 38
Taxonomy ......................................................................................................... 38
Genetic and Microbial Categories ................................................................. 38
Anatomic and Physiologic Characteristics .................................................. 39
Behavior ............................................................................................................ 39
Sexing ................................................................................................................ 40

APPENDIX E. BLOOD COLLECTION ...……………………………………........ 41
Orbital Sinus .................................................................................................... 41
Tail ..................................................................................................................... 41
Axillary Vessels ................................................................................................ 41
Intracardiac ....................................................................................................... 42

APPENDIX F. DRUG ADMINISTRATION ....………………………………........ 43
General Information ......................................................................................... 43
Injection Sites .................................................................................................... 43
Analgesics for Laboratory Animals ............................................................... 45
Anesthesia and Analgesia of Mice ................................................................. 46
Anesthesia and Analgesia of Rats ………………………………………….. 47
References for Anesthesia and Analgesia of Mice and Rats …………… 48




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