Biology DPP by Ambika Ma’am DPP
Biotechnology Principles and Processes
1. A limitation associated with traditional hybridisation 7. Which of the following is a biotechnological process?
procedures used in plant and animal breeding (1) IVF leading to ‘test tube baby’
overcome by genetic engineering is (2) Developing a DNA vaccine
(1) Traditional hybridisation is difficult to practice (3) Synthesising a gene and using it
(2) Traditional hybridisation is labour and cost (4) All of the above
intensive 8. Which of the following organelles is associated with
(3) Traditional hybridisation may lead to inclusion genetic engineering?
and multiplication of non desirable genes (1) Plasmids
(4) The progeny generated by traditional (2) Plastids
hybridisation have lower reproductive potential (3) Chloroplast
2. The scientists who cloned DNA from the Salmonella (4) Mitochondria
typhimurium streptomycin resistance plasmid into the 9. Which of the following is the most accepted definition of
Escherichia coli plasmid, observed tolerance to biotechnology by European Federation of Biotechnology?
streptomycin among the transformants and thus laying (1) Maintenance of sterile ambience for enabling
the foundations of genetic engineering were growth of desired microbe/eukaryotic cell in large
(1) Cohen and Boyer quantities
(2) Millstein and Kohler (2) Technique of using live organism or enzyme from
(3) Bolivar and Rodriguez organisms to produce products and processes
(4) Fire and Mello useful to animals
3. Production of transgenic plants is easier than (3) Process which use genetically engineered animals
production of transgenic animals mainly because only on a large scale for benefit of mankind
(1) Plant cells can grow in cell culture (4) The integration of natural science and organisms
(2) Plant cells have a lower number of potentially cells, parts thereof and molecular analogues for
lethal genes products and services
(3) Plant cells are totipotent 10. The transfer of genetic material from one bacterium to
(4) Plants do have cancers another through the mediation of a vector like virus is
4. Which of the following is not a feature of the plasmid? termed as
(1) Single stranded (1) Transfection
(2) Independent replication (2) Transduction
(3) Circular structure (3) Translation
(4) Small, circular double-stranded (4) Transformation
5. During ‘gene cloning’, which is called as gene taxi? 11. Genetic engineering is possible because
(1) Vaccine (2) Plasmid (1) DNA can be cut at specific sites by endonucleases
(3) Bacterium (4) Protozoa like DNAase
6. Hybridoma technology has been successfully used in (2) Restriction endonucleases purified from bacteria
(1) Production of somatic hybrids can be used invitro
(2) Synthesis of monoclonal antibodies (3) The phenomenon of transduction in bacteria is
(3) Synthesis of haemoglobin well understood
(4) Production of alcohol in bulk (4) DNA can be seen by electron microscope
1
, 12. Modern biotechnology consists 21. Restriction endonucleases are enzymes which
(1) Genetic engineering (2) Tissue culture (1) Remove nucleotides from the ends of the DNA molecule
(3) Microbiology (4) All of the above (2) Make cuts at specific positions within the DNA molecule
13. Plasmid is a (3) Recognise a specific nucleotide sequence for
(1) Fungus binding of DNA ligase
(2) Protozoans (4) Restrict the action of the enzyme DNA polymerase
(3) Part of plasma membrane 22. Identify the plasmid
(4) Extra chromosomal DNA in bacterial cell (1) pBR 322 (2) Hind II
14. Two microbes found to be very useful in genetic (3) EcoR I (4) AIU I
engineering are 23. Consider the following statements
(1) Escherichia coli and Agrobacterium tumefaciens I. Asexual reproduction preserves genetic information
(2) Vibrio cholerae and tailed bacteriophage while sexual reproduction permits variations
(3) Diplococcus sp. and Pseudomonas sp. II. Traditional hybridisation often leads to the inclusion
(4) None of the above and multiplication of undesirable genes along with
15. Transgenic plants are developed by the desired genes
(1) Introduction of foreign gene III. rDNA technology allows us to isolate and introduce
(2) Clone and genetically modified genes only one or a set of desirable genes without
(3) Purified genes introducing undesirable genes in the target organism
(4) None of the above Which of the above statements are true?
16. The EFB stands for (1) I & II only
(1) European Forum of Biotechnology (2) I & III only
(2) European Foundation of Biotechnology (3) II & III only
(3) European Function of Biotechnology (4) I, II, & III
(4) European Federation of Biotechnology 24. The enzyme that seals 5' PO4 and 3' OH polynucleotide
17. cDNA is ends while creating a recombinant DNA molecule is
(1) Circular DNA (1) Alkaline phosphatase
(2) Cloned DNA (2) DNA ligase
(3) DNA produced from reverse transcription of RNA (3) DNAse
(4) Cytoplasmic DNA (4) Restriction endonuclease
18. First recombinant was constructed in the year 25. Which of the following is a palindromic sequence?
(1) 1963 (2) 1974 (1) 5'-CGTATG-3’ (2) 5'-CGAATG-3’
(3) 1918 (4) 1972 3'-CGAATG-5’ 3'-GCATAC-5’
19. The cutting of DNA at specific locations became (3) 5'-GAATTC-3’ (4) 5'-GACTAC-3’
possible with the discovery of_____ 3'-CTTAAG-5’ 3'-CTTAAG-5'
(1) Restriction endonuclease enzymes 26. The most important feature present in a plasmid so it
(2) Probes can be used as a vector is
(3) Selectable markers (1) Origin of replication
(4) Ligases (2) Presence of sites for restriction endonuclease
20. Term ‘Restriction’ in restriction endonuclease enzyme (3) Presence of selectable marker
refers to (4) Presence of cloning sites
(1) Cleaving of phosphodiester bond in DNA by the 27. Which of the following features are required to
enzyme facilitate cloning into a vector?
(2) Prevention of bacteriophage multiplication in (i) Origin of replication (ori)
bacteria (ii) Selectable marker
(3) Cutting of DNA at specific position only (iii) Cloning site
(4) Cutting each of the two strands of DNA at specific (1) i & ii (2) ii & iii
points in sugar phosphate backbone (3) i & iii (4) i, ii & iii
2
Biotechnology Principles and Processes
1. A limitation associated with traditional hybridisation 7. Which of the following is a biotechnological process?
procedures used in plant and animal breeding (1) IVF leading to ‘test tube baby’
overcome by genetic engineering is (2) Developing a DNA vaccine
(1) Traditional hybridisation is difficult to practice (3) Synthesising a gene and using it
(2) Traditional hybridisation is labour and cost (4) All of the above
intensive 8. Which of the following organelles is associated with
(3) Traditional hybridisation may lead to inclusion genetic engineering?
and multiplication of non desirable genes (1) Plasmids
(4) The progeny generated by traditional (2) Plastids
hybridisation have lower reproductive potential (3) Chloroplast
2. The scientists who cloned DNA from the Salmonella (4) Mitochondria
typhimurium streptomycin resistance plasmid into the 9. Which of the following is the most accepted definition of
Escherichia coli plasmid, observed tolerance to biotechnology by European Federation of Biotechnology?
streptomycin among the transformants and thus laying (1) Maintenance of sterile ambience for enabling
the foundations of genetic engineering were growth of desired microbe/eukaryotic cell in large
(1) Cohen and Boyer quantities
(2) Millstein and Kohler (2) Technique of using live organism or enzyme from
(3) Bolivar and Rodriguez organisms to produce products and processes
(4) Fire and Mello useful to animals
3. Production of transgenic plants is easier than (3) Process which use genetically engineered animals
production of transgenic animals mainly because only on a large scale for benefit of mankind
(1) Plant cells can grow in cell culture (4) The integration of natural science and organisms
(2) Plant cells have a lower number of potentially cells, parts thereof and molecular analogues for
lethal genes products and services
(3) Plant cells are totipotent 10. The transfer of genetic material from one bacterium to
(4) Plants do have cancers another through the mediation of a vector like virus is
4. Which of the following is not a feature of the plasmid? termed as
(1) Single stranded (1) Transfection
(2) Independent replication (2) Transduction
(3) Circular structure (3) Translation
(4) Small, circular double-stranded (4) Transformation
5. During ‘gene cloning’, which is called as gene taxi? 11. Genetic engineering is possible because
(1) Vaccine (2) Plasmid (1) DNA can be cut at specific sites by endonucleases
(3) Bacterium (4) Protozoa like DNAase
6. Hybridoma technology has been successfully used in (2) Restriction endonucleases purified from bacteria
(1) Production of somatic hybrids can be used invitro
(2) Synthesis of monoclonal antibodies (3) The phenomenon of transduction in bacteria is
(3) Synthesis of haemoglobin well understood
(4) Production of alcohol in bulk (4) DNA can be seen by electron microscope
1
, 12. Modern biotechnology consists 21. Restriction endonucleases are enzymes which
(1) Genetic engineering (2) Tissue culture (1) Remove nucleotides from the ends of the DNA molecule
(3) Microbiology (4) All of the above (2) Make cuts at specific positions within the DNA molecule
13. Plasmid is a (3) Recognise a specific nucleotide sequence for
(1) Fungus binding of DNA ligase
(2) Protozoans (4) Restrict the action of the enzyme DNA polymerase
(3) Part of plasma membrane 22. Identify the plasmid
(4) Extra chromosomal DNA in bacterial cell (1) pBR 322 (2) Hind II
14. Two microbes found to be very useful in genetic (3) EcoR I (4) AIU I
engineering are 23. Consider the following statements
(1) Escherichia coli and Agrobacterium tumefaciens I. Asexual reproduction preserves genetic information
(2) Vibrio cholerae and tailed bacteriophage while sexual reproduction permits variations
(3) Diplococcus sp. and Pseudomonas sp. II. Traditional hybridisation often leads to the inclusion
(4) None of the above and multiplication of undesirable genes along with
15. Transgenic plants are developed by the desired genes
(1) Introduction of foreign gene III. rDNA technology allows us to isolate and introduce
(2) Clone and genetically modified genes only one or a set of desirable genes without
(3) Purified genes introducing undesirable genes in the target organism
(4) None of the above Which of the above statements are true?
16. The EFB stands for (1) I & II only
(1) European Forum of Biotechnology (2) I & III only
(2) European Foundation of Biotechnology (3) II & III only
(3) European Function of Biotechnology (4) I, II, & III
(4) European Federation of Biotechnology 24. The enzyme that seals 5' PO4 and 3' OH polynucleotide
17. cDNA is ends while creating a recombinant DNA molecule is
(1) Circular DNA (1) Alkaline phosphatase
(2) Cloned DNA (2) DNA ligase
(3) DNA produced from reverse transcription of RNA (3) DNAse
(4) Cytoplasmic DNA (4) Restriction endonuclease
18. First recombinant was constructed in the year 25. Which of the following is a palindromic sequence?
(1) 1963 (2) 1974 (1) 5'-CGTATG-3’ (2) 5'-CGAATG-3’
(3) 1918 (4) 1972 3'-CGAATG-5’ 3'-GCATAC-5’
19. The cutting of DNA at specific locations became (3) 5'-GAATTC-3’ (4) 5'-GACTAC-3’
possible with the discovery of_____ 3'-CTTAAG-5’ 3'-CTTAAG-5'
(1) Restriction endonuclease enzymes 26. The most important feature present in a plasmid so it
(2) Probes can be used as a vector is
(3) Selectable markers (1) Origin of replication
(4) Ligases (2) Presence of sites for restriction endonuclease
20. Term ‘Restriction’ in restriction endonuclease enzyme (3) Presence of selectable marker
refers to (4) Presence of cloning sites
(1) Cleaving of phosphodiester bond in DNA by the 27. Which of the following features are required to
enzyme facilitate cloning into a vector?
(2) Prevention of bacteriophage multiplication in (i) Origin of replication (ori)
bacteria (ii) Selectable marker
(3) Cutting of DNA at specific position only (iii) Cloning site
(4) Cutting each of the two strands of DNA at specific (1) i & ii (2) ii & iii
points in sugar phosphate backbone (3) i & iii (4) i, ii & iii
2
