Biotechnology and its Application
1. _______ triggers activation of protoxin to active toxin 6. Which of the following is not a biotechnological
of Bacillus thuringiensis in bollworm application of food production?
(1) Acidic pH of stomach (1) Apiculture
(2) Body temperature (2) Agro-chemical based agriculture
(3) Moist surface of midgut (3) Organic farming
(4) Alkaline pH of gut (4) Genetically engineered crop based agriculture
2. Which of the following Bt crops is being grown in India 7. The genetically modified (GM) brinjal in India has
by the farmers? been developed for
(1) Brinjal (1) Insect resistance
(2) Soyabean (2) Enhancing shelf life
(3) Maize (3) Enhancing mineral content
(4) Cotton (4) Drought resistance
3. Golden rice is a transgenic crop of the future with the 8. An improved variety of transgenic Basmati rice
following improved trait (1) Does not require chemical fertilisers and growth
(1) Insect resistance hormones
(2) High lysine (essential amino acid) content (2) Gives high yield and is rich in vitamin A
(3) High protein content (3) Is completely resistant to all insect pests and
(4) High vitamin A content diseases of paddy
4. Which of the following genes were introduced in (4) Gives high yield but has no characteristic aroma
cotton to protect it from cotton bollworm? 9. Antisense technology is
(1) Cry Ac and cry Ab (2) Bt Ac and Bt Ab (1) When a piece of RNA that is complementary in
(3) Cry IAc and cry II Ab sequence is used to stop expression of a specific gene
(4) Nif genes (2) RNA polymerase producing DNA
5. Match the following columns and select the correct (3) A cell displaying a foreign antigen used for
option synthesis of antigens
Column –I Column – II (4) Production of somaclonal variants in tissue cultures
(A) Btcotton (i) Gene therapy 10. Cry I endotoxins obtained from Bacillus thuringiensis
are effective against
(B) ADA (ii) Cellular defense
(1) Nematodes (2) Bollworms
(C) RNAi (iii) Detection of HIV Infection (3) Mosquitoes (4) Flies
(D) PCR (iv) Bacillus thuringiensis 11. Golden rice is a promising transgenic crop. When
released for cultivation, it will help in
(A) (B) (C) (D)
(1) Producing a petrol-like fuel from rice
(1) (iv) (i) (ii) (iii)
(2) Alleviation of vitamin A deficiency
(2) (iii) (ii) (i) (iv)
(3) Pest resistance
(3) (ii) (iii) (iv) (i)
(4) Herbicide tolerance
(4) (i) (ii) (iii) (iv)
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, 12. Bacillus thuringiensis has been used to kill certain 20. Choose the correct option regarding retrovirus
insects such as (1) A RNA virus that can synthesis DNA during infection
1. Tobacco budworm (2) A DNA virus that can synthesis RNA during infection
2. Armyworm 3. Beetles (3) A ssDNA virus
4. Flies 5. Mosquitoes (4) A dsRNA virus
(1) 1, 3 & 4 (2) 1, 2 & 5 21. Pathophysiology is the
(3) 1, 3, 4 & 5 (4) 1, 2, 3, 4 & 5 (1) Study of physiology of pathogen
13. The choice of the Bt-toxin genes depends upon the (2) Study of normal physiology of host
(1) Type of the crop (3) Study of altered physiology of host
(4) None of the above
(2) Type of targeted pest
22. Which of the following is commonly used as a vector for
(3) The type of vector
introducing a DNA fragment in human lymphocytes?
(4) Both (1) & (2)
(1) Retrovirus (2) Ti plasmid
14. Which of the following is the correct match?
(3) λ phage (4) pBR322
(1) CrylAb – Cotton bollworms
23. Agro-chemical based agriculture includes
(2) CrylAc – Cotton bollworms
(1) Fertilisers
(3) CrylAc – Corn borer (2) Pesticides
(4) Crylbc – Corn borer (3) Genetically modified crops
15. The main technique involved in agricultural (4) Both (1) & (2)
biotechnology is called 24. RNA interference is used for
(1) Tissue culture (1) To develop a pest resistant plant against
(2) Transformation infestation by nematode
(3) Plant breeding (2) To enhance the mineral usage by the plant
(4) DNA replication (3) To reduce post harvest losses
16. Strains of Bacillus thuringiensis are used in producing (4) To develop a plant tolerant to abiotic stresses
(1) Bioinsecticidal plants 25. Which of the following is true for Golden rice?
(2) Biominerals (1) It is vitamin A enriched, with a gene from daffodil
(3) Biometallurgical techniques (2) It is pest resistant with a gene from Bacillus
(4) Biofertilisers thuringiensis
17. A GM crop is transgenic. It can be harmful because it (3) It is drought tolerant, developed using
may cause agrobacterium vector
(1) Allergies and toxicity (4) It has yellow grains because of a gene introduced
(2) Incorporation of antibiotic resistance in human beings from a primitive variety of rice
26. Golden rice is a genetically modified crop plant where
(3) Disturbance in metabolism due to enzyme for
the incorporated gene is meant for biosynthesis of_____
antibiotic resistance
(1) Vitamin A (2) Vitamin B
(4) All of the above
(3) Vitamin C (4) Omega
18. Example of green biotechnology is
27. Which of the following Bt crop is being grown in India
(1) Industrial antibiotics
by the farmers?
(2) Industrial chemicals
(1) Cotton (2) Brinjal
(3) Industrial catalysts (3) Soyabean (4) Maize
(4) Bt corn and pest resistant plant 28. Some of the characteristics of Bt cotton are
19. Which enzyme is responsible for ripening of tomatoes? (1) High yield and resistance to bollworms
(1) β-galactosidase (2) Long fibre and resistance to aphids
(2) Polygalacturonase (3) Medium yield, long fibre and resistance to beetle pests
(3) Transferase (4) High yield and production of toxic protein
(4) Proteolase crystals which kill dipteran pests
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