MB (ASCP) Test Exam Questions And Answers Verified 100% Correct

MB (ASCP) Test Exam Questions And Answers Verified 100% Correct T-cell gene rearrangement is seen in what malignancy? - ANSWER Sezary Syndrome Which translocation causes Acute Premyelocytic Leukemia? - ANSWER t 15;17 Ewing Sarcoma - ANSWER t 11;22 Synovial Sarcoma - ANSWER t X;18 Follicular Lymphoma - ANSWER t 14;18 What genes would be screened in a colorectal panel? - ANSWER MSH2 & MLH1 What gene mutation causes a high risk of breast or ovarian cancer? - ANSWER BRCA1 and BRCA2 What does MSI test for? - ANSWER Mutations in mismatch repair genes (MMR) What disease is related to factor V coagulation gene (F5 1q23)? - ANSWER Thrombossis How many copies are in a full Fragile X mutation? - ANSWER 200-2000+ CGG repeats What disease is known for numerous mutations and large deletions? - ANSWER Muscular Dystrophy (Duchenne) What HPV causes cervical cancer? - ANSWER 16 & 18 You receive a paraffin embedded specimen for HPV typing, which method should you use? - ANSWER PCR Significant change in HIV viral load: - ANSWER 0.5 log10 Lowest viral concentration that can be detected over background or a negative control in the test assay - ANSWER Limit of Detection (LOD) HCV consensus sequence: - ANSWER G-rich Core Gene What responds poorly to ribavarin/interferon treatment? - ANSWER HCV Type 1B What virus affects patients after kidney transplant? - ANSWER CMV or BKV What exons are tested for HLA Class I? - ANSWER Exons 2 & 3 What exons are tested for HLA Class II? - ANSWER Exon 2 beta When sequencing HLD-DR what is targeted? - ANSWER Exon 2 beta What drug is metabolized by CYP2C19? - ANSWER Clopidogrel (Plavix) What drug tolerance is affected by CYP3A5? - ANSWER Carbamazepine What drug is metabolized by CYP2C9? - ANSWER Warfarin What drug is metabolized by CYP2D6? - ANSWER Codeine Mutation in UGT1A1 affectes metabolism of what drug? - ANSWER Irinotecan Antibiotic resistance mechanism in MRSA? - ANSWER mecA PBP2a resistance to methicillin Which has highest vancomycin resistance? - ANSWER vanA Warfarin causes inhibition of activities on which gene? - ANSWER VKORC1 Her2/neu exhibits an overexpression, what would this indicate? - ANSWER More aggressive growth and metastasis of the tumor cells in human breast cancer. Best condition for long term storage of RNA? (6 months) - ANSWER -70C ethanol How long can blood be stored at room temperature for DNA analysis? - ANSWER 24 hours Best method listed to collect bone marrow specimens: - ANSWER EDTA or ACD tubes How should whole blood be sent? - ANSWER Overnight on ice packs Blood should be stored and transported in: - ANSWER EDTA (purple/lavender top tubes) or ACD (yellow top tubes) How long can whole blood be at 4C before isolation? - ANSWER 72 hours How long to keep obsolete document in lab according to CAP checklist? - ANSWER 2 years If you break a phenol bottle, what should you do? - ANSWER Check MSDS then use spill kit When making 20% SDS, should you use a mask or not? - ANSWER Wear a face mask to avoid inhalation of SDS dust Clinical Sensitivity (SNIP) - ANSWER [TP/(TP+FN)] x 100 Clinical Specificity (SPIN) - ANSWER [TN/(TN+FP)] x 100 Where does the signal come from in pyrosequencing? - ANSWER Luciferase conversion of luciferin to oxyluciferin Primers are usually 18-30 bases, which PCR product should use a shorter primer? - ANSWER Previously amplified product What technique can use RNA or DNA as the starting substrate? - ANSWER bDNA What effect does methylation have on DNA? - ANSWER Inhibits transcription How do you increase the yield of shorter DNA products in PCR? - ANSWER Increase KCl concentration, decrease annealing temperature gradually What is P53? - ANSWER Tumor suppressor Which bonds hold together the bases in ssDNA? - ANSWER Phosphodiester bonds Which bonds hold together the nucleotides in dsDNA? - ANSWER Hydrogen bonds ATG CAG GTG (M Q V) ATG CAG GTT (M Q V) - ANSWER Silent ATG CAG GTG (M Q V)