Medical Laboratory Science..Med, MLS (ASC) & Final Microbiology, Clinical Laboratory Science Review Test (Micro), Clinical Microbiology Review Test Questions And Answers Verified 100% Correct

Medical Laboratory Science..Med, MLS (ASC) & Final Microbiology, Clinical Laboratory Science Review Test (Micro), Clinical Microbiology Review Test Questions And Answers Verified 100% Correct What is the historical medium of choice for Francisella - ANSWER Cysteine blood glucose agar is the historical medium of choice for ---- What type of hemolysis does S. aureus have - ANSWER Beta hemolytic Gram positive bacteria has which acid in the cell wall - ANSWER Lipoteichoic acid and Teichoic acid in the cell wall that prevents decolorization in the gram stain Gram positive bacteria is stained by what dye? - ANSWER Crystal violet Gram negative bacteria is stained by what dye? - ANSWER Safranin List the 4 types of media - ANSWER 1. Supportive 2. Enrichment 3. Selective 4. Differential Type of media: Supports growth of most non-fastidious bacteria - ANSWER Supportive Media, EX Nutrient Agar, TSA Type of media: Contains added growth factors, e.g., blood, vitamins, yeast extract - ANSWER Enrichment Media, EX: SBA Chocolate, Brain-Heart Infusion, Buffered charcoal-yeast extract agar Type of Media: Formulated to provide distinct colonial appearances based on certain biochemical rxn (e.g., lactose fermentation, hydrogen sulfide [H2S] production) - ANSWER Differential EX: EMB, MacConkey, HE, XLD Sheep blood agar (SBA) - ANSWER Media: Most Non-fastidious bacteria Chocolate agar (CHOC) - ANSWER Media: Haemophilus and Neisseria Columbia colistin-nalidixic acid agar (CNA) - ANSWER Media: GP Phenylethyl alcohol agar (PEA) - ANSWER Media: GPC and anaerobic GNR Group A-selective strep agar with 5% sheep blood (SSA) - ANSWER Media: Group A Strep from respiratory source Eosin methylene blue (EMB) - ANSWER Media: Enteric GNR MacConkey (MAC) agar - ANSWER Media: Enteric Gram Negative Rods Sorbitol MacConkey (SMAC) agar - ANSWER Media: E. Coli 0157h7 Hektoen enteric (HE) agar - ANSWER Salmonella & Shigellain stool Xylose lysine deoxycholate (XLD) - ANSWER Salmonella & Shigellain stool Salmonella-Shigella (SS) agar - ANSWER Salmonella & Shigellain stool Gram-negative broth (GN) - ANSWER Salmonella & Shigellafrom stools & rectal swabs Deoxycholate-citrate agar - ANSWER Salmonella & Shigella Campylobacterbroth - ANSWER Campylobacter from stool Campylobacterblood agar (Campy BAP) - ANSWER Campylobacter from stool Causes suppurative cutaneous infections, toxic shock syndrome, food poisoning - ANSWER S. aureus Opportunistic pathogen. Commoncause of hospital acquired UTI - ANSWER Coagulase-negative staphylococci (CNS) UTI in young sexually active females, urethritis & prostatitis in males - ANSWER S. saprophyticus Usually nonpathogenic. Found in environment & on skin, mucous membranes, oropharynx - ANSWER Micrococcus Enzyme catalase converts 3% hydrogen peroxide (H2O2) to oxygen & water. Immediate bubbling. - ANSWER Catalase Test Staphylococci pos. Streptococci & enterococci neg Enzyme coagulase causes coagulation (tube test) or agglutination (slide test) in plasma. - ANSWER Coagulase Test S. aureus pos Agglutination of latex beads coated with fibrinogen & abs to protein A (protein in cell wall of S. aureus). - ANSWER Slide agglutination tests for S. aureus S. aureus pos Fermentation of mannitol results in color change from pink to yellow. - ANSWER Mannitol salt agar (MSA) -7.5% salt inhibits most organisms other than staph. All staph can grow on MSA. Rarely used for ID of S. aureus today because other species ferment mannitol. Organisms resistant to novobiocin grow to edge of disk. - ANSWER Novobiocin susceptibility -S. saprophyticus is resistant. Other CNS susceptible Causes 90% of strep infections. Strep sore throat, rheumatic fever, glomerulonephritis, scarlet fever (scarlatina), erysipelas, puerperal sepsis, impetigo - ANSWER Group A streptococci (GAS) Sensitive to bacitracin. Resistant to SXT. PYR pos - ANSWER Most common is S. pyogenes. Hemolysi due to O2-stable streptolysin S & O2-labile streptolysin O. To detect species that produce streptolysin O only, stab into agar, place coverslip over inoculum, or incubate anaerobically Normal flora of female genital tract. Most common cause of neonatal septicemia & meningitis - ANSWER Group B streptococci (GBS) Resistant to SXT & bacitracin.