1
MCDB 165A EXAM LATEST UPDATES -2025/2026- ACTUAL
QUESTIONS WITH VERIFIED ANSWERS ALREADY GRADED A+
GUARANTEED SUCCESS
What do you use to pick cells up off a plate during passaging and how does it
work
trypsin, protease that digests the proteins helping the cell stick to the plate
What does formalin do?
reacts with side chains on amino acids and puts tissues into stasis, fixing the tissue
hematoxylin colors ____ ____
nuclei; blue
Who named cells
Robert Hooke
When did Robert Hooke identify cells?
1660s
, 2
When was the minimal essential medium for cell culture discovered?
1959
Type of serum commonly used for cell culturing
Fetal bovine serum
eosin colors ____ ____
cytoplasm; red
dye that fluorescently stains nuclei; interpolating agent, able to slide between
bases in DNA
DAPI
Where does DAPI stain
nucleus
Type of microscopy that uses a pinhole
confocal microscopy
, 3
Type of microscopy that uses evanescent wave to view the surface of an object
TIRF
List the components of a membrane phospholipid
two non-polar hydrophobic tails, glycerol, phosphate, variable group including
choline, ethanolamine, serine, or inositol
When did Gunter Blobel develop the signal sequence hypothesis?
1971
Describe the purpose of Valm et. al
applying systems-level spectral imaging and analysis to reveal the organelle
interactome
How did Valm and colleagues visualize organelles in the cell
fluorescent fusion proteins for each organelle and a dye for lipid droplets
what two types of microscopy are used in Valm et al
confocal and lattice light sheet
, 4
Protein/fluorescent protein combo used in Valm et. al for the lysosome
LAMP1-CFP
protein/fluorescence combo used in Valm et. al for mitochondria
mitochondrial targeting sequence of cytochrome c oxidase-eGFP
List the essential components of a plasmid in cloning
Origin of replication, promoter, selectable parker for antibiotic resistance (against
ampicillin), selectable marker to select against cells that did not take up the
plasmid when using eukaryotic organisms (puromycin), multi cloning site,
restriction sites
What media were the cells grown in in Valm et. al
Dulbecco's Modified Eagle's Medium (DMEM), 10% fetal bovine serum, 5% CO2
What are the 6 steps of an immunofluorescence experiment?
1. Fixation
2. Permeabilization (poking holes in membrane so antibodies can get through)
3. Blocking (prevent nonspecific binding due to stickiness with serum or dry milk)
MCDB 165A EXAM LATEST UPDATES -2025/2026- ACTUAL
QUESTIONS WITH VERIFIED ANSWERS ALREADY GRADED A+
GUARANTEED SUCCESS
What do you use to pick cells up off a plate during passaging and how does it
work
trypsin, protease that digests the proteins helping the cell stick to the plate
What does formalin do?
reacts with side chains on amino acids and puts tissues into stasis, fixing the tissue
hematoxylin colors ____ ____
nuclei; blue
Who named cells
Robert Hooke
When did Robert Hooke identify cells?
1660s
, 2
When was the minimal essential medium for cell culture discovered?
1959
Type of serum commonly used for cell culturing
Fetal bovine serum
eosin colors ____ ____
cytoplasm; red
dye that fluorescently stains nuclei; interpolating agent, able to slide between
bases in DNA
DAPI
Where does DAPI stain
nucleus
Type of microscopy that uses a pinhole
confocal microscopy
, 3
Type of microscopy that uses evanescent wave to view the surface of an object
TIRF
List the components of a membrane phospholipid
two non-polar hydrophobic tails, glycerol, phosphate, variable group including
choline, ethanolamine, serine, or inositol
When did Gunter Blobel develop the signal sequence hypothesis?
1971
Describe the purpose of Valm et. al
applying systems-level spectral imaging and analysis to reveal the organelle
interactome
How did Valm and colleagues visualize organelles in the cell
fluorescent fusion proteins for each organelle and a dye for lipid droplets
what two types of microscopy are used in Valm et al
confocal and lattice light sheet
, 4
Protein/fluorescent protein combo used in Valm et. al for the lysosome
LAMP1-CFP
protein/fluorescence combo used in Valm et. al for mitochondria
mitochondrial targeting sequence of cytochrome c oxidase-eGFP
List the essential components of a plasmid in cloning
Origin of replication, promoter, selectable parker for antibiotic resistance (against
ampicillin), selectable marker to select against cells that did not take up the
plasmid when using eukaryotic organisms (puromycin), multi cloning site,
restriction sites
What media were the cells grown in in Valm et. al
Dulbecco's Modified Eagle's Medium (DMEM), 10% fetal bovine serum, 5% CO2
What are the 6 steps of an immunofluorescence experiment?
1. Fixation
2. Permeabilization (poking holes in membrane so antibodies can get through)
3. Blocking (prevent nonspecific binding due to stickiness with serum or dry milk)
