HCT LE 4 Special Processing 3 & 4
1. Fixatives used in Histostain-SP kit: 6. Which of the following shows the proper order of the LAST
1 - 10% neutral buffered formalin 5 STEPS involved in the preparation of samples for electron
2 - 10% formol saline microscopy:
3 - Zinc formalin a. Sputter coating - freeze fracture - staining -
4 - B5 sectioning - trimming
a. 1 & 3 only b. Trimming - sectioning - freeze fracture - staining -
b. 2 & 4 only sputter coating
c. 1 , 3, & 4 only c. Trimming - sectioning - staining - sputter coating -
d. All of the choices freeze fracture
d. Trimming - sectioning - staining - freeze fracture -
2. Optimal incubation time for primary antibodies: sputter coating
a. 20-30 minutes at 20-22°C
b. 24 hours at 4°C 7. Analytical phase of QA includes:
c. 20-30 minutes at 37°C 1 - Treatment procedures
d. 24 hours at -4°C 2 - Visualization techniques
3 - Setting of (+) and (-) cut-off values
3. Nonspecific background staining may be reduced by 4 - Fixation methods
adding: a. 1 & 2 only
a. Diaminobenzidine b. 1 & 3 only
b. Formamide c. 2 & 4 only
c. Blocking serum d. All of the choices
d. Sodium azide
8. The following statements correctly describe internal tissue
4. The following statements describe slides under the direct controls, EXCEPT:
immunofluorescence technique: a. Contains the target antigen in adjacent normal tissue
1 - Stored at 4°C for 1 year element
2 - Orange-red fluorescence seen when fluorescein is used b. Examples include goat serum and IL-33
3 - Does not show decreased fluorescent staining through c. Also known as "built-in" control
time d. Eliminates the variable of tissue fixation
4 - Edges of coverslip are sealed using nail varnish
a. 1 & 3 only 9. Incubation time of specimens for ER/PR assay after the
b. 1 & 4 only addition of the secondary antibody
c. 2 & 4 only a. 24 hours at 56°C
d. 2 & 3only b. Room temperature for 4 minutes
e. 1 & 2.only c. 60 minutes at 25°C
d. 30 minutes at 25°C
5. Fixatives used in frozen section immunocytochemistry:
1 - Picric acid 10. Counterstain used in ER/PR:
2 - Acetone a. Light green
3 - Ethanol b. Methyl green
a. 1 , 2, & 3 c. Hematoxylin
b. 2 & 3 only d. Aniline blue
c. 1 & 3 only
d. 1 & 2 only 11. The following are correctly matched, EXCEPT:
a. ++ = 26 to 50% of tumor cells stained
b. +++ = 51 to75% of tumor cells stained
c. (staining intensity) +++ = intense labeling
d. (staining intensity) 0 = no labelling
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, HCT LE 4 Special Processing 3 & 4
12. Examples of secondary monoclonal antibodies: 17. Positive in lung adenocarcinoma:
1 - Goat anti-chicken lgY HRP a. EMA
2 - Goat anti-mouse lgG biotin b. CK7
3 - Rabbit anti-mouse lgG HRP c. PSA
4- Rabbit anti-mouse lgG H&l d. CK20
a. 2 & 3 only
b. 1 & 4 only 18. Tumor marker positive in ovarian CA:
c. 3 & 4 only 1 - CEA
d. 1 & 2only 2 - CA 125
3 - CA 15-3
13. Two-step indirect techniques involve: a. 1 only
1 - Addition of substrate-chromogen solutions b. 1,2,&3
2 - Elimination of cross-reactivity using pre-absorbed c. 1 & 3 only
primary anti-serum d. 1 & 2 only
3 - Binding of unconjugated primary antibody to the antigen
4 - Application of enzyme-labeled primary antibody against 19. Tumor marker positive in breast CA:
secondary antibody 1 - CK7
a. 2 & 4 only 2 - CEA
b. 1 & 2 only 3 - CA 15-3
c. 1, 2, & 3 a. 1 & 2 only
d. 1 & 3only b. 1, 2, & 3
c. 1 & 3 only
14. Positive in salivary gland tumors: d. 2 & 3.only
a. EMA
b. PSA 20. Disease associated with (+) keratin and (+) chromogranin:
c. TTF-1 a. Paraganglioma
d. CK-20 b. Leiomyoma
c. Ependymomas
15. Neuroendocrine markers: d. Neuroendocrine carcinoma
1 - Neurofilament
2 - Neuron-specific enolase 21 . Positive tumor marker in embryonal carcinoma:
3 - Chromogranin 1 - HCG
a. 1 & 2 only 2 - AFP
b. 1 & 3 only 3 - PLAP
c. 2 & 3 only a. 2 & 3 only
d. 1, 2, & 3 b. 1 & 3 only
c. 2 & 4 only
16. B-cell tumor markers: d. 1 & 4 only
1 - CD19
2 - CD15 22. Diseases associated with positive vimentin:
3 - CD20 1 - Undifferentiated sarcoma
4 - CD68 2 - Melanoma
a. 1 & 4 only 3 - Schwannoma
b. 1 & 3 only 4 - Rhabdomyosarcoma
c. 1 & 2 only a. 1 & 3 only
d. 2 & 4 only b. 2 & 3 only
c. 1,2,&3
d. All of the choices
This study source was downloaded by 100000899606396 from CourseHero.com on 07-08-2025 22:56:28 GMT -05:00
https://www.coursehero.com/file/249120811/Special-Processing-34pdf/
1. Fixatives used in Histostain-SP kit: 6. Which of the following shows the proper order of the LAST
1 - 10% neutral buffered formalin 5 STEPS involved in the preparation of samples for electron
2 - 10% formol saline microscopy:
3 - Zinc formalin a. Sputter coating - freeze fracture - staining -
4 - B5 sectioning - trimming
a. 1 & 3 only b. Trimming - sectioning - freeze fracture - staining -
b. 2 & 4 only sputter coating
c. 1 , 3, & 4 only c. Trimming - sectioning - staining - sputter coating -
d. All of the choices freeze fracture
d. Trimming - sectioning - staining - freeze fracture -
2. Optimal incubation time for primary antibodies: sputter coating
a. 20-30 minutes at 20-22°C
b. 24 hours at 4°C 7. Analytical phase of QA includes:
c. 20-30 minutes at 37°C 1 - Treatment procedures
d. 24 hours at -4°C 2 - Visualization techniques
3 - Setting of (+) and (-) cut-off values
3. Nonspecific background staining may be reduced by 4 - Fixation methods
adding: a. 1 & 2 only
a. Diaminobenzidine b. 1 & 3 only
b. Formamide c. 2 & 4 only
c. Blocking serum d. All of the choices
d. Sodium azide
8. The following statements correctly describe internal tissue
4. The following statements describe slides under the direct controls, EXCEPT:
immunofluorescence technique: a. Contains the target antigen in adjacent normal tissue
1 - Stored at 4°C for 1 year element
2 - Orange-red fluorescence seen when fluorescein is used b. Examples include goat serum and IL-33
3 - Does not show decreased fluorescent staining through c. Also known as "built-in" control
time d. Eliminates the variable of tissue fixation
4 - Edges of coverslip are sealed using nail varnish
a. 1 & 3 only 9. Incubation time of specimens for ER/PR assay after the
b. 1 & 4 only addition of the secondary antibody
c. 2 & 4 only a. 24 hours at 56°C
d. 2 & 3only b. Room temperature for 4 minutes
e. 1 & 2.only c. 60 minutes at 25°C
d. 30 minutes at 25°C
5. Fixatives used in frozen section immunocytochemistry:
1 - Picric acid 10. Counterstain used in ER/PR:
2 - Acetone a. Light green
3 - Ethanol b. Methyl green
a. 1 , 2, & 3 c. Hematoxylin
b. 2 & 3 only d. Aniline blue
c. 1 & 3 only
d. 1 & 2 only 11. The following are correctly matched, EXCEPT:
a. ++ = 26 to 50% of tumor cells stained
b. +++ = 51 to75% of tumor cells stained
c. (staining intensity) +++ = intense labeling
d. (staining intensity) 0 = no labelling
This study source was downloaded by 100000899606396 from CourseHero.com on 07-08-2025 22:56:28 GMT -05:00
https://www.coursehero.com/file/249120811/Special-Processing-34pdf/
, HCT LE 4 Special Processing 3 & 4
12. Examples of secondary monoclonal antibodies: 17. Positive in lung adenocarcinoma:
1 - Goat anti-chicken lgY HRP a. EMA
2 - Goat anti-mouse lgG biotin b. CK7
3 - Rabbit anti-mouse lgG HRP c. PSA
4- Rabbit anti-mouse lgG H&l d. CK20
a. 2 & 3 only
b. 1 & 4 only 18. Tumor marker positive in ovarian CA:
c. 3 & 4 only 1 - CEA
d. 1 & 2only 2 - CA 125
3 - CA 15-3
13. Two-step indirect techniques involve: a. 1 only
1 - Addition of substrate-chromogen solutions b. 1,2,&3
2 - Elimination of cross-reactivity using pre-absorbed c. 1 & 3 only
primary anti-serum d. 1 & 2 only
3 - Binding of unconjugated primary antibody to the antigen
4 - Application of enzyme-labeled primary antibody against 19. Tumor marker positive in breast CA:
secondary antibody 1 - CK7
a. 2 & 4 only 2 - CEA
b. 1 & 2 only 3 - CA 15-3
c. 1, 2, & 3 a. 1 & 2 only
d. 1 & 3only b. 1, 2, & 3
c. 1 & 3 only
14. Positive in salivary gland tumors: d. 2 & 3.only
a. EMA
b. PSA 20. Disease associated with (+) keratin and (+) chromogranin:
c. TTF-1 a. Paraganglioma
d. CK-20 b. Leiomyoma
c. Ependymomas
15. Neuroendocrine markers: d. Neuroendocrine carcinoma
1 - Neurofilament
2 - Neuron-specific enolase 21 . Positive tumor marker in embryonal carcinoma:
3 - Chromogranin 1 - HCG
a. 1 & 2 only 2 - AFP
b. 1 & 3 only 3 - PLAP
c. 2 & 3 only a. 2 & 3 only
d. 1, 2, & 3 b. 1 & 3 only
c. 2 & 4 only
16. B-cell tumor markers: d. 1 & 4 only
1 - CD19
2 - CD15 22. Diseases associated with positive vimentin:
3 - CD20 1 - Undifferentiated sarcoma
4 - CD68 2 - Melanoma
a. 1 & 4 only 3 - Schwannoma
b. 1 & 3 only 4 - Rhabdomyosarcoma
c. 1 & 2 only a. 1 & 3 only
d. 2 & 4 only b. 2 & 3 only
c. 1,2,&3
d. All of the choices
This study source was downloaded by 100000899606396 from CourseHero.com on 07-08-2025 22:56:28 GMT -05:00
https://www.coursehero.com/file/249120811/Special-Processing-34pdf/
