ASCP CG Certification Practice with correct answers
What is the best agent to use against mycoplasma? - correct answerBM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - correct
answercomparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - correct answerProteolytic enzymes - break down tissues
into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - correct answerColcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - correct answerLogged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - correct answerT-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is
the MOST important to document? - correct answerExpiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - correct
answerDissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - correct
answerDecidua (maternal tissue)
,When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around
the mets, what is the most likely cause? - correct answerinnappropriate slide drying time
What will help prevent microbial contamination and cross contamination of cultures? - correct
answerAsceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - correct answerprepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse,
what could help fix the issue? - correct answerIncreading the drying time by using warm slides
What is true of an inverted microscope? - correct answerthe objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - correct answerDiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - correct answerReason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
correct answerHeparinized peripheral blood stored at room temperature
(NOT FROZEN)
, A blood sample can be used to study the cytogenetics of a malignancy only if: - correct
answerDividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - correct answerTemperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast
scope is: - correct answerSlide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - correct answer45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? - correct answerChorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? - correct
answerThe door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up amniotic
fluid samples, the specimen could become contaminated with: - correct answerMycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain
used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination
What about the metaphases on a slide are affected by slide making? - correct answerCell
density - thru dilution of the sample with fix
What is the best agent to use against mycoplasma? - correct answerBM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - correct
answercomparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - correct answerProteolytic enzymes - break down tissues
into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - correct answerColcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - correct answerLogged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - correct answerT-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is
the MOST important to document? - correct answerExpiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - correct
answerDissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - correct
answerDecidua (maternal tissue)
,When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around
the mets, what is the most likely cause? - correct answerinnappropriate slide drying time
What will help prevent microbial contamination and cross contamination of cultures? - correct
answerAsceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - correct answerprepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse,
what could help fix the issue? - correct answerIncreading the drying time by using warm slides
What is true of an inverted microscope? - correct answerthe objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - correct answerDiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - correct answerReason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
correct answerHeparinized peripheral blood stored at room temperature
(NOT FROZEN)
, A blood sample can be used to study the cytogenetics of a malignancy only if: - correct
answerDividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - correct answerTemperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast
scope is: - correct answerSlide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - correct answer45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? - correct answerChorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? - correct
answerThe door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up amniotic
fluid samples, the specimen could become contaminated with: - correct answerMycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain
used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination
What about the metaphases on a slide are affected by slide making? - correct answerCell
density - thru dilution of the sample with fix
