ASCP CG (Classic Cytogenetics) Verified
Multiple Choice and Conceptual Actual
Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What does penicillin/streptomycin treat? - ANSWER Bacterial
contamination.
2. When do you use a kanamycin/gentamycin combination instead of other
antibiotics? - ANSWER When an organism is resistant to
penicillin/streptomycin. This combination is more broad-acting, but also a
more toxic antibiotic.
3. What do Amphotericin B, Fungizone and Mycostatin (Nystatin) treat? -
ANSWER Fungal contamination.
4. What temperature should a specimen be stored at short-term? - ANSWER
37 degrees C.
5. What temperature should a specimen be stored at long-term? - ANSWER
4 degrees C.
,6. True/False: Biological specimens are not required to be transported in leak
proof containers marked with bio-hazard labels. - ANSWER False.
7. True/False: When storage time of specimens increases, the viability of cells
decreases. - ANSWER True.
8. What is the optimal time frame between collection and culture that will
allow viable cells to grow? - ANSWER 1-2 days.
9. Why do we not culture a sample that was not stored in 4 degree C
conditions and collected a week ago? - ANSWER There will be a loss of
minor sub-populations and a low mitotic index.
10.How long can we store an uncultured specimen 4 degree C conditions? -
ANSWER 1 week.
11.When should we culture a bone marrow specimen? - ANSWER As soon
as possible.
12.Why do we culture a bone marrow specimen as soon as possible? -
ANSWER The removal of cells from the oxygen rich environment of the
body, along with the drop in temperature adversely affects cell division and
causes loss of minor sub-populations.
13.How do we store a bone marrow sample if it cannot be cultured within 4
hours? - ANSWER Add to media and store at room temp.
,14.True/False: Specimens should be shipped in direct contact with ice or dry
ice. - ANSWER False.
15.True/False: Temperatures over over 40 degrees C are lethal to cells and
tissues. - ANSWER True.
16.True/False: Solid tissues should be transported in sterile petri dishes or
conical tubes as soon as possible. - ANSWER True.
17.True/False: Many specimens can handled in a batch and stored in the same
containers. - ANSWER False.
18.True/False: Putting uncultured specimens in fixative is a good solution for
storage. - ANSWER False.
19.True/False: Specimens should not be transported under dry conditions as
this will result in a loss of cell viability. - ANSWER True.
20.What types of media can be used to preserve solid tissues? - ANSWER
RPMI 1640, 1x PBS, 1x Hank's solution, and isotonic saline.
21.What types of containers should be used to transport amniotic fluid
samples? - ANSWER Sterile, non-toxic syringes.
, 22.What needs to be added to the sterile medium in which a CVS sample is
being transported? - ANSWER 5-10% FBS and antibiotic.
23.How do you prevent bacterial contamination of a POC sample? - ANSWER
Rinse with media and antibiotics.
24.When do you not rinse solid tissues with antibiotics? - ANSWER When
it has been indicated that the specimen was transported with a cidal
concentration of antibiotic.
25.What type of media do amniotic fluid cultures grow best in? - ANSWER
Amnio-max.
26.What is peripheral blood composed of? - ANSWER Plasma, WBCs, and
RBCs.
27.What is the purpose of sodium heparin, lithuim heparin and EDTA in sample
transport? - ANSWER Anticoagulant.
28.Why is NaHep preferred over other anticoagulants? - ANSWER It is
generally non-toxic to lymphocytes.
29.What are the two classes of lymphocytes? - ANSWER T-cells and B-cells.
Multiple Choice and Conceptual Actual
Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What does penicillin/streptomycin treat? - ANSWER Bacterial
contamination.
2. When do you use a kanamycin/gentamycin combination instead of other
antibiotics? - ANSWER When an organism is resistant to
penicillin/streptomycin. This combination is more broad-acting, but also a
more toxic antibiotic.
3. What do Amphotericin B, Fungizone and Mycostatin (Nystatin) treat? -
ANSWER Fungal contamination.
4. What temperature should a specimen be stored at short-term? - ANSWER
37 degrees C.
5. What temperature should a specimen be stored at long-term? - ANSWER
4 degrees C.
,6. True/False: Biological specimens are not required to be transported in leak
proof containers marked with bio-hazard labels. - ANSWER False.
7. True/False: When storage time of specimens increases, the viability of cells
decreases. - ANSWER True.
8. What is the optimal time frame between collection and culture that will
allow viable cells to grow? - ANSWER 1-2 days.
9. Why do we not culture a sample that was not stored in 4 degree C
conditions and collected a week ago? - ANSWER There will be a loss of
minor sub-populations and a low mitotic index.
10.How long can we store an uncultured specimen 4 degree C conditions? -
ANSWER 1 week.
11.When should we culture a bone marrow specimen? - ANSWER As soon
as possible.
12.Why do we culture a bone marrow specimen as soon as possible? -
ANSWER The removal of cells from the oxygen rich environment of the
body, along with the drop in temperature adversely affects cell division and
causes loss of minor sub-populations.
13.How do we store a bone marrow sample if it cannot be cultured within 4
hours? - ANSWER Add to media and store at room temp.
,14.True/False: Specimens should be shipped in direct contact with ice or dry
ice. - ANSWER False.
15.True/False: Temperatures over over 40 degrees C are lethal to cells and
tissues. - ANSWER True.
16.True/False: Solid tissues should be transported in sterile petri dishes or
conical tubes as soon as possible. - ANSWER True.
17.True/False: Many specimens can handled in a batch and stored in the same
containers. - ANSWER False.
18.True/False: Putting uncultured specimens in fixative is a good solution for
storage. - ANSWER False.
19.True/False: Specimens should not be transported under dry conditions as
this will result in a loss of cell viability. - ANSWER True.
20.What types of media can be used to preserve solid tissues? - ANSWER
RPMI 1640, 1x PBS, 1x Hank's solution, and isotonic saline.
21.What types of containers should be used to transport amniotic fluid
samples? - ANSWER Sterile, non-toxic syringes.
, 22.What needs to be added to the sterile medium in which a CVS sample is
being transported? - ANSWER 5-10% FBS and antibiotic.
23.How do you prevent bacterial contamination of a POC sample? - ANSWER
Rinse with media and antibiotics.
24.When do you not rinse solid tissues with antibiotics? - ANSWER When
it has been indicated that the specimen was transported with a cidal
concentration of antibiotic.
25.What type of media do amniotic fluid cultures grow best in? - ANSWER
Amnio-max.
26.What is peripheral blood composed of? - ANSWER Plasma, WBCs, and
RBCs.
27.What is the purpose of sodium heparin, lithuim heparin and EDTA in sample
transport? - ANSWER Anticoagulant.
28.Why is NaHep preferred over other anticoagulants? - ANSWER It is
generally non-toxic to lymphocytes.
29.What are the two classes of lymphocytes? - ANSWER T-cells and B-cells.
