ASCP CG Certification Practice Verified Multiple Choice
and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is used to reduce mycoplasma contamination? - ANSWER BM
cycline
2. CLIA '88 stipulates that equipment must be recalibrated ____ ? - ANSWER
After a major repair to ensure still operational
3. How should caustic and corrosive chemicals be stored? - ANSWER Both
should be stored in a nonflammable cabinet
4. What factors would contribute to ALL cultures failing in a CO2 incubator? -
ANSWER Temperature
CO2 level
Humidity level
5. What is the modal number for a diploid karyotype and an additional CBG
negative marker chromosome? - ANSWER 46
CBG = c-bands achieved with barium with Giemsa stain
,CBG negative referrs to not having a centromere, which is crucial to being
included in the modal number, so this extra fragment does not have a
centromere and is not counted towards the total
6. What is included when recording the modal number? - ANSWER Mar -
a marker chromosome is included because it has a centromere
7. What is the BEST way to avoid producing ghost-like, pale chromosomes
while staining? - ANSWER Decrease trypsin time
8. In order to ensure successful harvest, each new batch of hypotonic solution
should be evaluated ____. - ANSWER By comparing new hypotonic
solution to that already in use
9. Which cell type would you want to use for long term serial cytogenetic
studies? - ANSWER Fibroblast cells
10.What is not a necessary (must be added) ingredient which is added to
modified medium? - ANSWER Antibiotics
11.What chemical is used for cryopreservation? - ANSWER Dimethyl
sulfoxide (DMSO)
12.You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - ANSWER NOR
, 13.When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - ANSWER Propidium
iodide (red)
14.A bone marrow sample collected in transport medium is leaking into the
sealed plastic bag in which it was transported. The technologist
should_______. - ANSWER Clean up the tube while wearing gloves
15.A laboratory' quality assurance program includes _______________. -
ANSWER Proficiency testing
16.All of the following chromosomes have dark bands on chromosome regions
that can be used as indicators when preparing the best image: - ANSWER
Chromosome 11p
Chromosome 12q
Chromosome 17q
17.The morning after being set-up, a single dish of an amniotic fluid culture
turns cloudy and appears contaminated. None of the other cultures set-up
that day appears contaminated. What should be done first? - ANSWER
Harvest the culture as soon as possible
18.When the addition of nonhomologous material to the q-arm of
chromosome 16 is suspected after G-banding, what banding technique
should be used? - ANSWER C-banding
and Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is used to reduce mycoplasma contamination? - ANSWER BM
cycline
2. CLIA '88 stipulates that equipment must be recalibrated ____ ? - ANSWER
After a major repair to ensure still operational
3. How should caustic and corrosive chemicals be stored? - ANSWER Both
should be stored in a nonflammable cabinet
4. What factors would contribute to ALL cultures failing in a CO2 incubator? -
ANSWER Temperature
CO2 level
Humidity level
5. What is the modal number for a diploid karyotype and an additional CBG
negative marker chromosome? - ANSWER 46
CBG = c-bands achieved with barium with Giemsa stain
,CBG negative referrs to not having a centromere, which is crucial to being
included in the modal number, so this extra fragment does not have a
centromere and is not counted towards the total
6. What is included when recording the modal number? - ANSWER Mar -
a marker chromosome is included because it has a centromere
7. What is the BEST way to avoid producing ghost-like, pale chromosomes
while staining? - ANSWER Decrease trypsin time
8. In order to ensure successful harvest, each new batch of hypotonic solution
should be evaluated ____. - ANSWER By comparing new hypotonic
solution to that already in use
9. Which cell type would you want to use for long term serial cytogenetic
studies? - ANSWER Fibroblast cells
10.What is not a necessary (must be added) ingredient which is added to
modified medium? - ANSWER Antibiotics
11.What chemical is used for cryopreservation? - ANSWER Dimethyl
sulfoxide (DMSO)
12.You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining the
chromosomal origin of this marker? - ANSWER NOR
, 13.When using a fluorescein dye (yellow-green) labeled probe during the FISH
procedure, which counterstain should be used? - ANSWER Propidium
iodide (red)
14.A bone marrow sample collected in transport medium is leaking into the
sealed plastic bag in which it was transported. The technologist
should_______. - ANSWER Clean up the tube while wearing gloves
15.A laboratory' quality assurance program includes _______________. -
ANSWER Proficiency testing
16.All of the following chromosomes have dark bands on chromosome regions
that can be used as indicators when preparing the best image: - ANSWER
Chromosome 11p
Chromosome 12q
Chromosome 17q
17.The morning after being set-up, a single dish of an amniotic fluid culture
turns cloudy and appears contaminated. None of the other cultures set-up
that day appears contaminated. What should be done first? - ANSWER
Harvest the culture as soon as possible
18.When the addition of nonhomologous material to the q-arm of
chromosome 16 is suspected after G-banding, what banding technique
should be used? - ANSWER C-banding
