ASCP CG (Classic Cytogenetics) Verified
Multiple Choice and Conceptual Actual
Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What type of media do amniotic fluid cultures grow best in? - ANSWER
Amnio-max.
2. What is peripheral blood composed of? - ANSWER Plasma, WBCs, and
RBCs.
3. What is the purpose of sodium heparin, lithuim heparin and EDTA in sample
transport? - ANSWER Anticoagulant.
4. Why is NaHep preferred over other anticoagulants? - ANSWER It is
generally non-toxic to lymphocytes.
5. What are the two classes of lymphocytes? - ANSWER T-cells and B-cells.
6. What mitogens do T-cells respond to? - ANSWER PHA and Concanavalin
A.
,7. What mitogens do B-cells respond to? - ANSWER LPS, Epstein-Barr
Virus, and TPA.
8. Which mitogen stimulates both T and B cells? - ANSWER Pokeweed.
9. What group of mitogens is used to stimulate cells with a constitutional
abnormality? - ANSWER T-cell mitogens.
10.How do we stimulate the growth of neoplastic cells or B-cells neoplasms? -
ANSWER Run one unstimulated culture and one with a B-cell mitogen.
11.How are percutaneous umbilical blood samples (PUBS) retrieved? -
ANSWER Transabdominally with the assistance of ultrasonography.
12.What is the turnaround time for an amniocentesis culture? - ANSWER
7-14 days.
13.What is the turnaround time for a fetal blood culture? - ANSWER 2-3
days.
14.What is the earliest a PUB can be performed? - ANSWER 18 weeks.
15.When was amniocentesis first reported? - ANSWER 1880s
,16.What did Steele and Breg do in 1966? - ANSWER They used
amniocentesis for cytogenetic analysis.
17.What is the risk for maternal cell contamination? - ANSWER 0.3% risk.
18.What is maternal cell contamination mostly attributed to? - ANSWER
Failure to discard the 2 cc of amniotic fluid and generally bloody samples.
19.What is the ideal gestational age for a specimen tap? - ANSWER 16-20
weeks.
20.Why are early and late specimen taps not ideal for culture? - ANSWER
The cultures will be slow growing.
21.What do we do with the remaining amniotic fluid that is not used for
culturing cells? - ANSWER We send it to another department for AFP
testing.
22.Which takes longer to grow in a culture: solid tissue or bone marrow? -
ANSWER Solid tissue.
23.What is the most common issue with solid tissue culture? - ANSWER
Microbial contamination?
, 24.What did Hahnemann and Mohr develop in 1968? - ANSWER Chorionic
Villus Sampling (CVS).
25.How are CVS samples retrieved? - ANSWER Transcervically and
transabdominally.
26.How do we reduce cellular contamination of a chorionic villus sample? -
ANSWER By removing the adherent maternal decidua.
27.Describe the physical differences between villi and decidua. - ANSWER
The villi will be branched in appearance and lighter as compared to the
decidua, which will be more sheet-like and darker.
28.What is the prerequisite for using solid tissues in cytogenetic study? -
ANSWER That the cells have the ability to divide.
29.Why do we remove necrotic tissue and normal parenchyma from the tissue
before setting a culture? - ANSWER Necrotic tissue increases the
toxicity of the culture, and normal cells will typically outgrow the abnormal
cells in this environment.
30.What fluids can we use to study metastatic cells? - ANSWER Pleural and
abdominal ascites fluids.
Multiple Choice and Conceptual Actual
Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What type of media do amniotic fluid cultures grow best in? - ANSWER
Amnio-max.
2. What is peripheral blood composed of? - ANSWER Plasma, WBCs, and
RBCs.
3. What is the purpose of sodium heparin, lithuim heparin and EDTA in sample
transport? - ANSWER Anticoagulant.
4. Why is NaHep preferred over other anticoagulants? - ANSWER It is
generally non-toxic to lymphocytes.
5. What are the two classes of lymphocytes? - ANSWER T-cells and B-cells.
6. What mitogens do T-cells respond to? - ANSWER PHA and Concanavalin
A.
,7. What mitogens do B-cells respond to? - ANSWER LPS, Epstein-Barr
Virus, and TPA.
8. Which mitogen stimulates both T and B cells? - ANSWER Pokeweed.
9. What group of mitogens is used to stimulate cells with a constitutional
abnormality? - ANSWER T-cell mitogens.
10.How do we stimulate the growth of neoplastic cells or B-cells neoplasms? -
ANSWER Run one unstimulated culture and one with a B-cell mitogen.
11.How are percutaneous umbilical blood samples (PUBS) retrieved? -
ANSWER Transabdominally with the assistance of ultrasonography.
12.What is the turnaround time for an amniocentesis culture? - ANSWER
7-14 days.
13.What is the turnaround time for a fetal blood culture? - ANSWER 2-3
days.
14.What is the earliest a PUB can be performed? - ANSWER 18 weeks.
15.When was amniocentesis first reported? - ANSWER 1880s
,16.What did Steele and Breg do in 1966? - ANSWER They used
amniocentesis for cytogenetic analysis.
17.What is the risk for maternal cell contamination? - ANSWER 0.3% risk.
18.What is maternal cell contamination mostly attributed to? - ANSWER
Failure to discard the 2 cc of amniotic fluid and generally bloody samples.
19.What is the ideal gestational age for a specimen tap? - ANSWER 16-20
weeks.
20.Why are early and late specimen taps not ideal for culture? - ANSWER
The cultures will be slow growing.
21.What do we do with the remaining amniotic fluid that is not used for
culturing cells? - ANSWER We send it to another department for AFP
testing.
22.Which takes longer to grow in a culture: solid tissue or bone marrow? -
ANSWER Solid tissue.
23.What is the most common issue with solid tissue culture? - ANSWER
Microbial contamination?
, 24.What did Hahnemann and Mohr develop in 1968? - ANSWER Chorionic
Villus Sampling (CVS).
25.How are CVS samples retrieved? - ANSWER Transcervically and
transabdominally.
26.How do we reduce cellular contamination of a chorionic villus sample? -
ANSWER By removing the adherent maternal decidua.
27.Describe the physical differences between villi and decidua. - ANSWER
The villi will be branched in appearance and lighter as compared to the
decidua, which will be more sheet-like and darker.
28.What is the prerequisite for using solid tissues in cytogenetic study? -
ANSWER That the cells have the ability to divide.
29.Why do we remove necrotic tissue and normal parenchyma from the tissue
before setting a culture? - ANSWER Necrotic tissue increases the
toxicity of the culture, and normal cells will typically outgrow the abnormal
cells in this environment.
30.What fluids can we use to study metastatic cells? - ANSWER Pleural and
abdominal ascites fluids.
