ASCP (CG Mock) Verified Multiple Choice
and Conceptual Actual Emended Exam
Questions With Reviewed 100% Correct
Detailed Answers
Guaranteed Pass!!Current Update
1. Which of the cellular organelles is most effected by Colchicine?
a. Cell membrane
b. Ribosomes
c. Nuclear envelope
d. Spindle apparatus - ANSWER Spindle apparatus - causes cells to
freeze at the metaphase
2. Mitotic arrestants prevent cells from entering?
a. S phase
b. Prophase
c. Anaphase
d. Telophase - ANSWER Anaphase
3. How many grams of potassium chloride is added to 1000ml of double
deionized water to make 0.075M KCL?
a. 5.58g
b. 2.79g
c. 4.79g
d. 7.50g - ANSWER 5.58g
,4. Which of the following statements BEST describes a hypotonic solution?
a. A solution with a higher salt concentration than the cell cytoplasm
b. A solution with a lower salt concentration than the cell cytoplasm
c. A solution with a salt concentration equal to the cell cytoplasm
d. A solution used to wash cells - ANSWER A solution with a lower
salt concentration than the cell cytoplasm
5. Which of the following is NOT an effect of fixative?
a. Denatures and removes H20 from proteins
b. Causes the cell membrane to transport to shut down. No more water
can enter or leave the cell
c. Softens the cell membrane
d. Kills the cell - ANSWER Soften the cell membrane
6. Carnoy's Fixative is used during the harvest procedure. Which of the
following is the standard ration of methanol to acetic acid; in the order
stated?
a. 1:1
b. 1:2
c. 2:1
d. 3:1
e. 1:3 - ANSWER 3:1
7. If the technologists wants to ensue the maximum spreading of the
chromosomes during harvest, what measures could be taken?
a. Increase the concentration of mitotic arresting agent
b. Use a very warm hypotonic solution (43 'C)
c. Use warm hypotonic solution (37 'C)
, d. Use room temperature hypotonic solution (20 'C) - ANSWER Use
warm hypotonic solution (37 'C)
8. A specimen was processed for cytogenetic studies. The culture, harvest,
slide preparation and staining procedures went along without unusual
incidents. Seven slides were dropped and five were stained. However, upon
microscopic analysis, all cells observed were interphase cells, no mitotic
cells were present. Other specimens harvested on the same day had fair to
good mitotic index. What is the appropriate course of action?
a. Obtain new specimen for repeat studies
b. Retrieve the fixed suspension and "drop" additional slides for analysis
c. Retrieve the fixed suspension and "drop" additional increasing the
amount of fixative
d. Stain remaining slides and analyze again - ANSWER Retrieve the
fixed suspension and "drop" additional slides for analysis
9. Cytoplasm surrounding chromosomes on newly dropped slides?
a. Will cause a reduction in quality of banding
b. Will have no effect on banding procedures
c. Is normal phenomenon
d. Should be avoided at all costs as properly dropped slides will be
completely free of any cytoplasm - ANSWER Will cause a
reduction in quality of banding
10.When newly dropped slides are observed, the chromosomes appear to be
nonuniform. The chromosomes vary in quality not only within the cell but
also across the slide. Which of the following would cause this unevenness?
a. Dirty slides
b. Nonuniform drying of the slide
c. Air currents
, d. All of the above - ANSWER All of the above
11.Short chromosomes will result from?
a. A high concentration of Colcemid
b. A short exposure to Colcemid
c. A short exposure to hypotonic solution
d. Improper fixation - ANSWER A high concentration of Colcemid
12.Which of the following techniques can be used to increase chromosome
spreading?
a. Decreasing the hypotonic time
b. Dropping the cells from an increased height
c. Increasing the concentration of the suspension
d. All of the above - ANSWER Dropping the cells from an increased
height
13.A technologist is having a problem with cytoplasm remaining around the
cells. Two extra fixative steps have been added, but the problem persists
and the cell pellet is white and fluffy appearance. What else can this
technologist do to alleviate this problem?
a. Manipulate the drying time
b. Decrease the methanol concentration
c. Wash two more times with fixative
d. Drop the cell suspension onto the slide from the lesser height -
ANSWER Wash two more times with fixative
14.Which of the following statements about G-banding are FALSE?
a. Methanol-acetic acid fixative is necessary for chromosome spreading
but has no affect on G-banding.
and Conceptual Actual Emended Exam
Questions With Reviewed 100% Correct
Detailed Answers
Guaranteed Pass!!Current Update
1. Which of the cellular organelles is most effected by Colchicine?
a. Cell membrane
b. Ribosomes
c. Nuclear envelope
d. Spindle apparatus - ANSWER Spindle apparatus - causes cells to
freeze at the metaphase
2. Mitotic arrestants prevent cells from entering?
a. S phase
b. Prophase
c. Anaphase
d. Telophase - ANSWER Anaphase
3. How many grams of potassium chloride is added to 1000ml of double
deionized water to make 0.075M KCL?
a. 5.58g
b. 2.79g
c. 4.79g
d. 7.50g - ANSWER 5.58g
,4. Which of the following statements BEST describes a hypotonic solution?
a. A solution with a higher salt concentration than the cell cytoplasm
b. A solution with a lower salt concentration than the cell cytoplasm
c. A solution with a salt concentration equal to the cell cytoplasm
d. A solution used to wash cells - ANSWER A solution with a lower
salt concentration than the cell cytoplasm
5. Which of the following is NOT an effect of fixative?
a. Denatures and removes H20 from proteins
b. Causes the cell membrane to transport to shut down. No more water
can enter or leave the cell
c. Softens the cell membrane
d. Kills the cell - ANSWER Soften the cell membrane
6. Carnoy's Fixative is used during the harvest procedure. Which of the
following is the standard ration of methanol to acetic acid; in the order
stated?
a. 1:1
b. 1:2
c. 2:1
d. 3:1
e. 1:3 - ANSWER 3:1
7. If the technologists wants to ensue the maximum spreading of the
chromosomes during harvest, what measures could be taken?
a. Increase the concentration of mitotic arresting agent
b. Use a very warm hypotonic solution (43 'C)
c. Use warm hypotonic solution (37 'C)
, d. Use room temperature hypotonic solution (20 'C) - ANSWER Use
warm hypotonic solution (37 'C)
8. A specimen was processed for cytogenetic studies. The culture, harvest,
slide preparation and staining procedures went along without unusual
incidents. Seven slides were dropped and five were stained. However, upon
microscopic analysis, all cells observed were interphase cells, no mitotic
cells were present. Other specimens harvested on the same day had fair to
good mitotic index. What is the appropriate course of action?
a. Obtain new specimen for repeat studies
b. Retrieve the fixed suspension and "drop" additional slides for analysis
c. Retrieve the fixed suspension and "drop" additional increasing the
amount of fixative
d. Stain remaining slides and analyze again - ANSWER Retrieve the
fixed suspension and "drop" additional slides for analysis
9. Cytoplasm surrounding chromosomes on newly dropped slides?
a. Will cause a reduction in quality of banding
b. Will have no effect on banding procedures
c. Is normal phenomenon
d. Should be avoided at all costs as properly dropped slides will be
completely free of any cytoplasm - ANSWER Will cause a
reduction in quality of banding
10.When newly dropped slides are observed, the chromosomes appear to be
nonuniform. The chromosomes vary in quality not only within the cell but
also across the slide. Which of the following would cause this unevenness?
a. Dirty slides
b. Nonuniform drying of the slide
c. Air currents
, d. All of the above - ANSWER All of the above
11.Short chromosomes will result from?
a. A high concentration of Colcemid
b. A short exposure to Colcemid
c. A short exposure to hypotonic solution
d. Improper fixation - ANSWER A high concentration of Colcemid
12.Which of the following techniques can be used to increase chromosome
spreading?
a. Decreasing the hypotonic time
b. Dropping the cells from an increased height
c. Increasing the concentration of the suspension
d. All of the above - ANSWER Dropping the cells from an increased
height
13.A technologist is having a problem with cytoplasm remaining around the
cells. Two extra fixative steps have been added, but the problem persists
and the cell pellet is white and fluffy appearance. What else can this
technologist do to alleviate this problem?
a. Manipulate the drying time
b. Decrease the methanol concentration
c. Wash two more times with fixative
d. Drop the cell suspension onto the slide from the lesser height -
ANSWER Wash two more times with fixative
14.Which of the following statements about G-banding are FALSE?
a. Methanol-acetic acid fixative is necessary for chromosome spreading
but has no affect on G-banding.
