ASCP Verified Multiple Choice and Conceptual
Actual Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is the most appropriate anticoagulant for collecting peripheral blood
for karyotyping or FISH?
A. EDTA
B. Heparin
C. Citrate
D. Oxalate
Answer: B. Heparin
2. Why is EDTA NOT recommended for cytogenetic studies?
A. It causes hemolysis
B. It disrupts chromosome morphology
C. It does not prevent clotting
D. It enhances cell proliferation
Answer: B. It disrupts chromosome morphology
3. Which of the following is a critical pre-analytical factor that can affect the
success of karyotyping and FISH?
A. Type of microscope used
B. Temperature during transportation
C. Color of the test tube
D. Use of immersion oil
Answer: B. Temperature during transportation
,4. For prenatal diagnosis, the first few milliliters of amniotic fluid should be
discarded to prevent:
A. Chromosome degradation
B. Maternal cell contamination
C. Cell clumping
D. Bacterial overgrowth
Answer: B. Maternal cell contamination
5. What is the maximum recommended time between sample collection and
culture setup for optimal karyotyping results?
A. 1 hour
B. 12 hours
C. 24–48 hours
D. 5 days
Answer: C. 24–48 hours
6. What volume of amniotic fluid is generally sufficient for cytogenetic analysis
(karyotyping and FISH)?
A. 2–5 mL
B. 5–10 mL
C. 10–20 mL
D. 15–30 mL
Answer: D. 15–30 mL
7. What is the purpose of using sodium heparin in cytogenetic blood collection
tubes?
A. To stain chromosomes
B. To inhibit bacterial growth
C. To prevent clotting and preserve metaphase cells
, D. To lyse red blood cells
Answer: C. To prevent clotting and preserve metaphase cells
8. What is the ideal state of tissue culture before harvesting for FISH or
karyotyping?
A. Fully confluent
B. 25% confluent
C. 50–75% confluent
D. Detached from the surface
Answer: C. 50–75% confluent
9. In FISH, what is the purpose of fixation with Carnoy's fixative prior to
hybridization?
A. To enhance fluorescent signals
B. To lyse cells
C. To remove bacterial contamination
D. To preserve cellular morphology and permeabilize membranes
Answer: D. To preserve cellular morphology and permeabilize membranes
10. For solid tissue samples, what is the best practice to reduce microbial
contamination before culture?
A. Heat-fix the tissue
B. Add EDTA to the transport media
C. Rinse with ethanol
D. Use transport media with antibiotics and antifungals
Answer: D. Use transport media with antibiotics and antifungals
1. What is the preferred anticoagulant for peripheral blood samples intended for
cytogenetic analysis?
Actual Emended Exam Questions With Reviewed
100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is the most appropriate anticoagulant for collecting peripheral blood
for karyotyping or FISH?
A. EDTA
B. Heparin
C. Citrate
D. Oxalate
Answer: B. Heparin
2. Why is EDTA NOT recommended for cytogenetic studies?
A. It causes hemolysis
B. It disrupts chromosome morphology
C. It does not prevent clotting
D. It enhances cell proliferation
Answer: B. It disrupts chromosome morphology
3. Which of the following is a critical pre-analytical factor that can affect the
success of karyotyping and FISH?
A. Type of microscope used
B. Temperature during transportation
C. Color of the test tube
D. Use of immersion oil
Answer: B. Temperature during transportation
,4. For prenatal diagnosis, the first few milliliters of amniotic fluid should be
discarded to prevent:
A. Chromosome degradation
B. Maternal cell contamination
C. Cell clumping
D. Bacterial overgrowth
Answer: B. Maternal cell contamination
5. What is the maximum recommended time between sample collection and
culture setup for optimal karyotyping results?
A. 1 hour
B. 12 hours
C. 24–48 hours
D. 5 days
Answer: C. 24–48 hours
6. What volume of amniotic fluid is generally sufficient for cytogenetic analysis
(karyotyping and FISH)?
A. 2–5 mL
B. 5–10 mL
C. 10–20 mL
D. 15–30 mL
Answer: D. 15–30 mL
7. What is the purpose of using sodium heparin in cytogenetic blood collection
tubes?
A. To stain chromosomes
B. To inhibit bacterial growth
C. To prevent clotting and preserve metaphase cells
, D. To lyse red blood cells
Answer: C. To prevent clotting and preserve metaphase cells
8. What is the ideal state of tissue culture before harvesting for FISH or
karyotyping?
A. Fully confluent
B. 25% confluent
C. 50–75% confluent
D. Detached from the surface
Answer: C. 50–75% confluent
9. In FISH, what is the purpose of fixation with Carnoy's fixative prior to
hybridization?
A. To enhance fluorescent signals
B. To lyse cells
C. To remove bacterial contamination
D. To preserve cellular morphology and permeabilize membranes
Answer: D. To preserve cellular morphology and permeabilize membranes
10. For solid tissue samples, what is the best practice to reduce microbial
contamination before culture?
A. Heat-fix the tissue
B. Add EDTA to the transport media
C. Rinse with ethanol
D. Use transport media with antibiotics and antifungals
Answer: D. Use transport media with antibiotics and antifungals
1. What is the preferred anticoagulant for peripheral blood samples intended for
cytogenetic analysis?
