ASCP - Molecular Cytogenetic Testing: FISH and
Microarray Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. Name several types of FISH probes - ANSWER painting , alpha satellite ,
beta satellite , locus specific
2. What are painting probes? - ANSWER probes that hybridize to a whole
chromosome or chromosome arm
3. How are painting probes useful? - ANSWER in detecting marker
chromosomes, translocations, and anueploidies in metaphase
chromosomes
4. What are alpha satellite probes? - ANSWER probes that hybridize to
repetitive dna located at the centromere of chromosomes
5. How are alpha satellite probes useful? - ANSWER in detecting
aneuploidy of specific chromosomes in both metaphase and interphase
cells
,6. What are beta satellite probes or NOR probes? - ANSWER probes that
hybridize to the repetitive dna on the short arms of the acrocentric
chromosomes
7. What are classical satellite probes? - ANSWER located in the
heterochromatic regions of chromosomes 1,9,16 and Y
8. What are telomere probes? - ANSWER probes that hybridize to the
terminal ends of both the p and q arms of chromosomes
9. What are locus specific probes? - ANSWER unique sequence probes
that hybridize to specific genes or gene clusters.
10.Dual color single fusion probe - ANSWER 1F1R1G
11.Dual color extra signal probes - ANSWER 1F2R1G
12.Dual color dual fusion probe - ANSWER 2F1R1G
13.Dual color/break apart rearrangement probe - ANSWER a normal
configuration is denoted by a yellow signal pattern (2F). If there is a
rearrangement or translocation, the signal pattern is 1F1R1G
, 14.Why is it important that the metaphases and interphases are free of
cytoplasm and debris? - ANSWER This can interfere with probe
hybidization and cause an increase in background signal
15.How should FISH slides be pretreated if there is a continuance of excess
cytoplasmic background? - ANSWER acid wash or proteinase K
16.How should non-chromosome preparations for FISH (smears, cytospins) be
pretreated? - ANSWER 1:1 glacial acetic acid and methanol for 2
minutes to lyse any red blood cells present. Then place in fresh 1:1 glacial
acetic acid and methanol for an additional 5 minutes
17.How are FISH slides routinely stored? - ANSWER At room temp in a dust
free box
18.How are slides that are less than 2 wks. old artificially aged? - ANSWER
In 2xSSC (pH 7.0) @ 37 degrees celsius for 30 minutes. Then dehydrate in
70, 85, 100 percent ethanol for two minutes each @ room temp.
19.At what temp may FISH slides be stored if they cannot be processed right
away? - ANSWER -20 degrees celsius for up to six months
20.FISH slides can be stored at what temp. for up to one year? - ANSWER -
80 degrees celsius
Microarray Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
1. Name several types of FISH probes - ANSWER painting , alpha satellite ,
beta satellite , locus specific
2. What are painting probes? - ANSWER probes that hybridize to a whole
chromosome or chromosome arm
3. How are painting probes useful? - ANSWER in detecting marker
chromosomes, translocations, and anueploidies in metaphase
chromosomes
4. What are alpha satellite probes? - ANSWER probes that hybridize to
repetitive dna located at the centromere of chromosomes
5. How are alpha satellite probes useful? - ANSWER in detecting
aneuploidy of specific chromosomes in both metaphase and interphase
cells
,6. What are beta satellite probes or NOR probes? - ANSWER probes that
hybridize to the repetitive dna on the short arms of the acrocentric
chromosomes
7. What are classical satellite probes? - ANSWER located in the
heterochromatic regions of chromosomes 1,9,16 and Y
8. What are telomere probes? - ANSWER probes that hybridize to the
terminal ends of both the p and q arms of chromosomes
9. What are locus specific probes? - ANSWER unique sequence probes
that hybridize to specific genes or gene clusters.
10.Dual color single fusion probe - ANSWER 1F1R1G
11.Dual color extra signal probes - ANSWER 1F2R1G
12.Dual color dual fusion probe - ANSWER 2F1R1G
13.Dual color/break apart rearrangement probe - ANSWER a normal
configuration is denoted by a yellow signal pattern (2F). If there is a
rearrangement or translocation, the signal pattern is 1F1R1G
, 14.Why is it important that the metaphases and interphases are free of
cytoplasm and debris? - ANSWER This can interfere with probe
hybidization and cause an increase in background signal
15.How should FISH slides be pretreated if there is a continuance of excess
cytoplasmic background? - ANSWER acid wash or proteinase K
16.How should non-chromosome preparations for FISH (smears, cytospins) be
pretreated? - ANSWER 1:1 glacial acetic acid and methanol for 2
minutes to lyse any red blood cells present. Then place in fresh 1:1 glacial
acetic acid and methanol for an additional 5 minutes
17.How are FISH slides routinely stored? - ANSWER At room temp in a dust
free box
18.How are slides that are less than 2 wks. old artificially aged? - ANSWER
In 2xSSC (pH 7.0) @ 37 degrees celsius for 30 minutes. Then dehydrate in
70, 85, 100 percent ethanol for two minutes each @ room temp.
19.At what temp may FISH slides be stored if they cannot be processed right
away? - ANSWER -20 degrees celsius for up to six months
20.FISH slides can be stored at what temp. for up to one year? - ANSWER -
80 degrees celsius
