ASCP CG Exam (Molecular CG Testing) Verified
Multiple Choice and Conceptual Actual Emended
Exam Questions With Reviewed 100%
Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is the main purpose of using FISH in cytogenetics?
a) To detect protein expression
b) To visualize specific DNA sequences on chromosomes
c) To amplify DNA fragments
d) To isolate RNA
Answer: b) To visualize specific DNA sequences on chromosomes
2. Which of the following would be used as a FISH probe to detect
aneuploidy?
a) Telomere probe
b) Locus-specific probe
c) Centromere (alpha satellite) probe
d) Painting probe
Answer: c) Centromere (alpha satellite) probe
3. Which of the following conditions is commonly diagnosed using a 22q11.2
deletion FISH probe?
a) Turner Syndrome
b) Klinefelter Syndrome
c) DiGeorge Syndrome
d) Patau Syndrome
Answer: c) DiGeorge Syndrome
4. What is the purpose of using formamide during FISH?
a) Enhances probe fluorescence
, b) Maintains pH balance
c) Lowers DNA melting temperature
d) Preserves tissue morphology
Answer: c) Lowers DNA melting temperature
Microarray (aCGH / SNP Array)
5. What is a primary advantage of chromosomal microarray (CMA) over
traditional karyotyping?
a) Detects balanced translocations
b) Identifies small copy number variations (CNVs)
c) Is faster to perform
d) Requires metaphase spreads
Answer: b) Identifies small copy number variations (CNVs)
6. Which type of microarray uses oligonucleotide probes for detecting CNVs?
a) SNP array
b) CGH array
c) Expression array
d) BAC array
Answer: b) CGH array
7. Which of the following abnormalities cannot be detected by CMA?
a) Microdeletions
b) Uniparental disomy
c) Balanced translocations
d) Copy number duplications
Answer: c) Balanced translocations
Hybridization & Probe Design
8. What factor most affects hybridization specificity during FISH?
a) Fluorophore brightness
Multiple Choice and Conceptual Actual Emended
Exam Questions With Reviewed 100%
Correct Detailed Answers
Guaranteed Pass!!Current Update
1. What is the main purpose of using FISH in cytogenetics?
a) To detect protein expression
b) To visualize specific DNA sequences on chromosomes
c) To amplify DNA fragments
d) To isolate RNA
Answer: b) To visualize specific DNA sequences on chromosomes
2. Which of the following would be used as a FISH probe to detect
aneuploidy?
a) Telomere probe
b) Locus-specific probe
c) Centromere (alpha satellite) probe
d) Painting probe
Answer: c) Centromere (alpha satellite) probe
3. Which of the following conditions is commonly diagnosed using a 22q11.2
deletion FISH probe?
a) Turner Syndrome
b) Klinefelter Syndrome
c) DiGeorge Syndrome
d) Patau Syndrome
Answer: c) DiGeorge Syndrome
4. What is the purpose of using formamide during FISH?
a) Enhances probe fluorescence
, b) Maintains pH balance
c) Lowers DNA melting temperature
d) Preserves tissue morphology
Answer: c) Lowers DNA melting temperature
Microarray (aCGH / SNP Array)
5. What is a primary advantage of chromosomal microarray (CMA) over
traditional karyotyping?
a) Detects balanced translocations
b) Identifies small copy number variations (CNVs)
c) Is faster to perform
d) Requires metaphase spreads
Answer: b) Identifies small copy number variations (CNVs)
6. Which type of microarray uses oligonucleotide probes for detecting CNVs?
a) SNP array
b) CGH array
c) Expression array
d) BAC array
Answer: b) CGH array
7. Which of the following abnormalities cannot be detected by CMA?
a) Microdeletions
b) Uniparental disomy
c) Balanced translocations
d) Copy number duplications
Answer: c) Balanced translocations
Hybridization & Probe Design
8. What factor most affects hybridization specificity during FISH?
a) Fluorophore brightness
