BIO 171 LAB EXAMS 1-3 EXAM WITH
CORRECT QUESTIONS AND ANSWERS
2025
If you are working in a lab in which an autoclave is not available, and you are
pressed for time, which would you choose to best sterilize your equipment?
Hot steam or hot air? Explain why you chose your answer. - CORRECT-
ANSWERSHot steam is the best choice as you can achieve a sterile
environment in a matter of minutes whereas hot air will take several hours to
achieve the same effect.
What three elements are used in an autoclave to sterilize equipment? -
CORRECT-ANSWERSHeat, pressure, and steam.
What type of incubator is pictured below? - CORRECT-ANSWERSFixed
incubator
What is the minimum temperature an autoclave must be set at to achieve
sterile condition? - CORRECT-ANSWERS125°C
, At what temperature is the fixed incubator set to, as presented in the lab
video? - CORRECT-ANSWERS37°C
What are the FOUR types of gloves presented in the lab video? - CORRECT-
ANSWERSLatex, Nitrile, Thermal cold, Thermal heat
What are the main sections that should be found in a lab notebook? Name at
least 4. - CORRECT-ANSWERSObjective, Procedure, Notes, Results and
Deviations
At what temperature should you refrigerate bacterial samples? Explain why
this is beneficial. - CORRECT-ANSWERS4°C. This temperature slows bacterial
growth and prolongs the life of the sample.
What THREE rules were discussed in regards to lab safety that would protect
you and others from contamination? - CORRECT-ANSWERS1. Never eat or
drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
CORRECT QUESTIONS AND ANSWERS
2025
If you are working in a lab in which an autoclave is not available, and you are
pressed for time, which would you choose to best sterilize your equipment?
Hot steam or hot air? Explain why you chose your answer. - CORRECT-
ANSWERSHot steam is the best choice as you can achieve a sterile
environment in a matter of minutes whereas hot air will take several hours to
achieve the same effect.
What three elements are used in an autoclave to sterilize equipment? -
CORRECT-ANSWERSHeat, pressure, and steam.
What type of incubator is pictured below? - CORRECT-ANSWERSFixed
incubator
What is the minimum temperature an autoclave must be set at to achieve
sterile condition? - CORRECT-ANSWERS125°C
, At what temperature is the fixed incubator set to, as presented in the lab
video? - CORRECT-ANSWERS37°C
What are the FOUR types of gloves presented in the lab video? - CORRECT-
ANSWERSLatex, Nitrile, Thermal cold, Thermal heat
What are the main sections that should be found in a lab notebook? Name at
least 4. - CORRECT-ANSWERSObjective, Procedure, Notes, Results and
Deviations
At what temperature should you refrigerate bacterial samples? Explain why
this is beneficial. - CORRECT-ANSWERS4°C. This temperature slows bacterial
growth and prolongs the life of the sample.
What THREE rules were discussed in regards to lab safety that would protect
you and others from contamination? - CORRECT-ANSWERS1. Never eat or
drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
