BIS 102 Questions and Answers Already Passed
Q - AnswersGlutamine (1 letter)
E - AnswersGlutamic Acid (1 letter)
Y - AnswersTyrosine (1 letter)
W - AnswersTryptophan (1 letter)
K - AnswersLysine (1 letter)
N - AnswersAsparagine (1 letter)
D - AnswersAspartic Acid (1 letter)
T - AnswersThreonine (1 letter)
Imidazole - AnswersHistidine side chain, five-membered aromatic ring with 2N atoms
6.5 - AnswerspKa side chain imidazole
3 - AnswerspKa C-terminus carboxyl
8 - AnswerspKa N-terminus amino group
11 - AnswerspKa side chain amine
pH<pKa - AnswersProtonated
pH>pKa - AnswersDeprotonated
+/- 0.5 - AnswersCharge when pH=pKa (sign dependent on molecule)
Charge of C-terminus carboxyl when deprotonated - Answers-1
Charge of groups other than C-terminus when deprotonated - Answers0
Charge of C-terminus carboxyl when protonated - Answers0
Charge of groups other than C-terminus carboxyl when protonated - Answers1
SDS-PAGE - Answersseparates proteins by size/molecular weight, determines approx number of amino
acids
Size exclusion chromatography - Answersdetermines the molecular size of a protein's native state
Ion exchange chromatography - Answersseparates proteins by net charge, partially purifies a basic
protein from a mixture of mostly acidic proteins
Q - AnswersGlutamine (1 letter)
E - AnswersGlutamic Acid (1 letter)
Y - AnswersTyrosine (1 letter)
W - AnswersTryptophan (1 letter)
K - AnswersLysine (1 letter)
N - AnswersAsparagine (1 letter)
D - AnswersAspartic Acid (1 letter)
T - AnswersThreonine (1 letter)
Imidazole - AnswersHistidine side chain, five-membered aromatic ring with 2N atoms
6.5 - AnswerspKa side chain imidazole
3 - AnswerspKa C-terminus carboxyl
8 - AnswerspKa N-terminus amino group
11 - AnswerspKa side chain amine
pH<pKa - AnswersProtonated
pH>pKa - AnswersDeprotonated
+/- 0.5 - AnswersCharge when pH=pKa (sign dependent on molecule)
Charge of C-terminus carboxyl when deprotonated - Answers-1
Charge of groups other than C-terminus when deprotonated - Answers0
Charge of C-terminus carboxyl when protonated - Answers0
Charge of groups other than C-terminus carboxyl when protonated - Answers1
SDS-PAGE - Answersseparates proteins by size/molecular weight, determines approx number of amino
acids
Size exclusion chromatography - Answersdetermines the molecular size of a protein's native state
Ion exchange chromatography - Answersseparates proteins by net charge, partially purifies a basic
protein from a mixture of mostly acidic proteins
