Microbiology Lab Final Exam Version 2 Actual Exam
MICROBIOLOGY LAB FINAL EXAM VERSION 2 ACTUAL EXAM
NEWEST COMPLETE ALL QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) |ALREADY GRADED A+||BRAND
NEW VERSION!
Several of the bacteria discussed (acinetobacterium, alcaligenes, agrobacterium,
pseudomonas) are gram-negative, obligate respires, that can grow aerobically and
vary in their ability to grow by anaerobic respiration. Which of the following
results would NOT be consistent with any of these bacteria?
a. growth only at the top of the thiogylcolate test
b. positive catalase test
c. yellow color in glucose fermentation
d. white or pale colony on MacConkeys - ANSWER-c
the best criterion for sterilization is
a. 99.9% removal of viable microorganisms
b. lack of pathogenic microbes
c. a 1000-fold reduction in the number of microbes
d. no viable endospores - ANSWER-d
what methods is/are often used to sterilize heat-sensitive items? check all that
apply
a. autoclaving
b. filtration
c. adding antibiotics
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, Microbiology Lab Final Exam Version 2 Actual Exam
d. irradiation - ANSWER-b & d
Which of the following are consistent with good "aseptic technique"?
a. all of them
b. placing the top lids of petri dishes on teh table top while making transfers
c. leaving culture tubes unplugged and upright in the test tube rack while making
inoculations between them
d. holding the test tube cap with your pinkie finger not in the palm of your hand
e. touching the wire of a recently-flame-sterilized inoculating loop to your hand to
check if it is cool enough to touch the loop to the inoculum - ANSWER-d
What is the best description of a primary research article?
a. credibility is difficult to ascertain
b. probably written for a wide audience
c. most likely to be a reliable source of information
d. a blog written by an individual
e. a review article in a speciality scientific journal - ANSWER-c
The microbes discussed in this course are introduced by providing their basic
characteristics. As you learned about these microbes, what are the basic
characteristics that seem to be universally important (or nearly so) in describing
the basic features of a bacterial species? check all that apply
a. oxygen relationship
b. how fast they grow
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, Microbiology Lab Final Exam Version 2 Actual Exam
c. gram stain
d. how they cause disease
(what is the free response answer too?) - ANSWER-c
(typically: gram type, cell morphology, and oxygen relationship)
what is the most likely reason why we cannot develop a universal medium that is
able to support the growth of all bacteria?
a. actually there is one
b. it would require too many ingredients
c. it would require too much work
d. we do not know all the requirements for most bacteria on earth - ANSWER-d
Robert Koch developed his set of postulates to show that
a. can be universally applied to ALL diseases
b. a specific microbe causes a specific disease
c. vaccination was possible
d. microbes were the cause of wine spoilage - ANSWER-b
Steps of Koch's Postulate - ANSWER-1. microbe agent always present in ill animal,
and not in healthy animal
2. microbe must be isolated in pure culture
3. isolated microbe must cause same disease in a healthy animal
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, Microbiology Lab Final Exam Version 2 Actual Exam
4. microbe must be re-isolated from second animal
Which of these is NOT needed to demonstrate Koch's postulate?
a. susceptible host animal
b. media to grow the suspected microbe in pure culture
c. animals that lack the suspected microbe
d. an attenuated pathogen for comparison
e. a human counter part to the disease - ANSWER-d & e
Which of the following are situations where we cannot use Koch's Postulates?
Check all that apply.
a. diseases that are limited to human host
b. diseases caused by the "joint" efforts" of more then one microbe
c. diseases that are only caused by bacteria
d. diseases caused by pathogen we cannot grow in the lab - ANSWER-a, b, & d
What are biochemical properties we can use to distinguish Erwinia cartovora from
E. coli
a. presence of pectinase
b. lactose fermentation
c. none of these, they are indistinguishable.
d. Gram reaction
e. catalase negative - ANSWER-a & e
4|Page
MICROBIOLOGY LAB FINAL EXAM VERSION 2 ACTUAL EXAM
NEWEST COMPLETE ALL QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) |ALREADY GRADED A+||BRAND
NEW VERSION!
Several of the bacteria discussed (acinetobacterium, alcaligenes, agrobacterium,
pseudomonas) are gram-negative, obligate respires, that can grow aerobically and
vary in their ability to grow by anaerobic respiration. Which of the following
results would NOT be consistent with any of these bacteria?
a. growth only at the top of the thiogylcolate test
b. positive catalase test
c. yellow color in glucose fermentation
d. white or pale colony on MacConkeys - ANSWER-c
the best criterion for sterilization is
a. 99.9% removal of viable microorganisms
b. lack of pathogenic microbes
c. a 1000-fold reduction in the number of microbes
d. no viable endospores - ANSWER-d
what methods is/are often used to sterilize heat-sensitive items? check all that
apply
a. autoclaving
b. filtration
c. adding antibiotics
1|Page
, Microbiology Lab Final Exam Version 2 Actual Exam
d. irradiation - ANSWER-b & d
Which of the following are consistent with good "aseptic technique"?
a. all of them
b. placing the top lids of petri dishes on teh table top while making transfers
c. leaving culture tubes unplugged and upright in the test tube rack while making
inoculations between them
d. holding the test tube cap with your pinkie finger not in the palm of your hand
e. touching the wire of a recently-flame-sterilized inoculating loop to your hand to
check if it is cool enough to touch the loop to the inoculum - ANSWER-d
What is the best description of a primary research article?
a. credibility is difficult to ascertain
b. probably written for a wide audience
c. most likely to be a reliable source of information
d. a blog written by an individual
e. a review article in a speciality scientific journal - ANSWER-c
The microbes discussed in this course are introduced by providing their basic
characteristics. As you learned about these microbes, what are the basic
characteristics that seem to be universally important (or nearly so) in describing
the basic features of a bacterial species? check all that apply
a. oxygen relationship
b. how fast they grow
2|Page
, Microbiology Lab Final Exam Version 2 Actual Exam
c. gram stain
d. how they cause disease
(what is the free response answer too?) - ANSWER-c
(typically: gram type, cell morphology, and oxygen relationship)
what is the most likely reason why we cannot develop a universal medium that is
able to support the growth of all bacteria?
a. actually there is one
b. it would require too many ingredients
c. it would require too much work
d. we do not know all the requirements for most bacteria on earth - ANSWER-d
Robert Koch developed his set of postulates to show that
a. can be universally applied to ALL diseases
b. a specific microbe causes a specific disease
c. vaccination was possible
d. microbes were the cause of wine spoilage - ANSWER-b
Steps of Koch's Postulate - ANSWER-1. microbe agent always present in ill animal,
and not in healthy animal
2. microbe must be isolated in pure culture
3. isolated microbe must cause same disease in a healthy animal
3|Page
, Microbiology Lab Final Exam Version 2 Actual Exam
4. microbe must be re-isolated from second animal
Which of these is NOT needed to demonstrate Koch's postulate?
a. susceptible host animal
b. media to grow the suspected microbe in pure culture
c. animals that lack the suspected microbe
d. an attenuated pathogen for comparison
e. a human counter part to the disease - ANSWER-d & e
Which of the following are situations where we cannot use Koch's Postulates?
Check all that apply.
a. diseases that are limited to human host
b. diseases caused by the "joint" efforts" of more then one microbe
c. diseases that are only caused by bacteria
d. diseases caused by pathogen we cannot grow in the lab - ANSWER-a, b, & d
What are biochemical properties we can use to distinguish Erwinia cartovora from
E. coli
a. presence of pectinase
b. lactose fermentation
c. none of these, they are indistinguishable.
d. Gram reaction
e. catalase negative - ANSWER-a & e
4|Page
