exam WITH COMPLETE COMPREHENSIVE
QUESTIONS AND SURE VERIFIED SOLUTIONS
100%
1. Which of the four elements is proteins differentiates this class substances fr
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om carbohydrates and lipids?
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A. carbon
B. hydrogen
C. nitrogen
D. oxygen: C. Nitrogen
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2. Which of the proteins is soluble in water?
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A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins: A. Albumin SW SW
3. How are amino acids joined together to form a chainlike structure?
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A. disulfide bonding SW
B. hydrogen bonding SW
C. covalent bonding SW
D. peptide bonding: D. Peptide bonding
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4. Proteins may become denatured when subjected to mechanical agitation, h
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eat, or chemical treatment. What does denaturation of proteins refer to?
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A. amino acid substitution
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B. addition of a prosthetic group
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C. alteration in tertiary structure SW SW SW
D. increase in solubility: C. Alteration in tertiary structure
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,5. Which aminoacidopathy is NOT caused by an enzyme deficiency?
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A. maple syrup substitution
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B. phenylketonuria
C. alkaptonuria
D. cystinuria: D. Cystinuria
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6. How many immunoglobulin classes are currently recognized?
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A. two
B. three
C. four
D. five: D. Five
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,7. Which reagent is employed in the serum protein determination?
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A. biuret
B. molybdenum blue SW
C. ferricferrocyanide
D. diacetyl monoxime: A. Biuret SW SW SW
8. Why should hemolyzed serum not be used for total protein analysis?
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A. hemoglobin is absorbed at the same wavelength as protein in the reagent
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B. there are elevated protein amounts in red blood cells as compared to serum
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C. hemoglobin reacts with biuret reagent SW SW SW SW
D. the value will be falsely lowered: A. Hemoglobin is absorbed at the same w
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avelength as protein in the reagent
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9. Which of the following is NOT true of the biuret reaction?
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A. follows Beer's Law for a reasonable range of protein
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B. depends on the presence of at least two peptide linkages
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C. is relatively free from interference by lipids and hemoglobin
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D. is insensitive to the low protein levels of urine: C. is relatively free from i
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nterference by lipids and hemoglobin
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10. Why is bromcresol purple the preferred indicator for albumin dye binding
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techniques?
A. it lends itself to manual and automated procedures
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B. it never bind with alpha or beta globulins
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C. it can be employed with both serum and plasma
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D. there is less interference from pigment(s): D. there is less interference from p
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igment(s)
11. What is the most anodic protein on electrophoresis at pH 8.6?
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, A. albumin
B. alpha-globulins
C. beta-globulins
D. gamma-globulins: A. albumin SW SW
12. The total protein is 7.2. The serum protein results are as follows:
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Albumin 53% 3.8 g/dL SW SW SW
Alpha-1 3.6% 0.3 g/dL SW SW SW
Alpha-2 11% 0.8 g/dL SW SW SW
Betta 17.6% 1.2 g/dL SW SW SW
Gamma 14.8% 1.1 g/dL Wha SW SW SW SW
t is the A/G ratio?
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A. 3.4
B. 1.6
C. 1.2
D. 1.1: D. 1.1 SW SW
13. A serum protein electrophoretic pattern displaying a beta gamma bridge
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or broad gamma is seen in which disease state?
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A. acute inflammation SW
B. monoclonal gammopathy SW
C. active cirrhosis SW
D. nephrotic syndrome: C. Active cirrhosis SW SW SW SW
14. If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-
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globulin, 4%-10% beta- SW SW
globulin, and 45% gamma globulin, then what condition may exist in the pati
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ent?
A. cirrhosis
B. inflammation
C. nephrotic syndrome SW
D. monoclonal gammopathy: D. Monoclonal gammopathy SW SW SW SW
15. Glycoproteins and mucoproteins are usually bound to which substance? SW SW SW SW SW SW SW SW
A. lipids
B. nucleic acids SW
C. carbohydrates
D. metals: C. Carbohydrates SW SW
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