UTA Micro 2460 Exam 2026 Questions
and Answers
Binary Fission - Answer -Most common form of bacterial reproduction
Binary Fission
4Basic Steps - Answer -1.Growth of cell size and increase in cell components
2.Replication of DNA
3.Division of the cytoplasm (cytokinesis)
4.Septum formation and division of daughter cells
Z ring Assembly - Answer -•Cytokinesis is directed by FtsZ protein
•FtsZ assembles Z ring to form divisome
•Divisome activates production of peptidoglycan and septum
Generation Time - Answer -•Generation Time (Doubling Time) - time takes to
double population
Generation Time
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,among species - Answer -•E. coli = 20 min. S. aureus = 30 min.
B. subtilis = 120 min.M. tuberculosis = 15-20 hrs
Calculating Population Size - Answer -•Growth is exponential (if resources are no
concern)
•Population can be predicted from any starting size
Nn= N02n
Nn - number of cells at generation n
n - number of generations
N0 - initial number of cells
•
•Number of generation may need to be calculated
•Ex. Generation time of 30 min = 48 generations in 24 hours
1 initial cell would result in more than 281 trillion cells after 1 day!
Growth Curve - Answer -•Closed cultures have finite resources (i.e. nutrients)
Culture density - Answer -the number of cells per unit volume
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, growth curve phases - Answer -lag phase, log phase, stationary phase, death phase
1.Lag phase - Answer -- inoculum cells added and adjust to culture medium; no
change in population
2.Log - Answer -(exponential) phase - binary fission occurs;cell replication > cell
death
3.Stationary phase - Answer -resources become depleted cell replication = cell
death
4.Death phase - Answer -1.Death phase - endospores can form cell replication <
cell death
Lag Phase - Answer -•Initial cell numbers do not change
•Cells grow larger; metabolically active
•Damaged or shocked cells undergo repair
•Duration of the lag phase determined by many factors including:
•Genetic make-up
•Media composition
•Initial inoculum size
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and Answers
Binary Fission - Answer -Most common form of bacterial reproduction
Binary Fission
4Basic Steps - Answer -1.Growth of cell size and increase in cell components
2.Replication of DNA
3.Division of the cytoplasm (cytokinesis)
4.Septum formation and division of daughter cells
Z ring Assembly - Answer -•Cytokinesis is directed by FtsZ protein
•FtsZ assembles Z ring to form divisome
•Divisome activates production of peptidoglycan and septum
Generation Time - Answer -•Generation Time (Doubling Time) - time takes to
double population
Generation Time
©COPYRIGHT 2025, ALL RIGHTS RESERVED 1
,among species - Answer -•E. coli = 20 min. S. aureus = 30 min.
B. subtilis = 120 min.M. tuberculosis = 15-20 hrs
Calculating Population Size - Answer -•Growth is exponential (if resources are no
concern)
•Population can be predicted from any starting size
Nn= N02n
Nn - number of cells at generation n
n - number of generations
N0 - initial number of cells
•
•Number of generation may need to be calculated
•Ex. Generation time of 30 min = 48 generations in 24 hours
1 initial cell would result in more than 281 trillion cells after 1 day!
Growth Curve - Answer -•Closed cultures have finite resources (i.e. nutrients)
Culture density - Answer -the number of cells per unit volume
©COPYRIGHT 2025, ALL RIGHTS RESERVED 2
, growth curve phases - Answer -lag phase, log phase, stationary phase, death phase
1.Lag phase - Answer -- inoculum cells added and adjust to culture medium; no
change in population
2.Log - Answer -(exponential) phase - binary fission occurs;cell replication > cell
death
3.Stationary phase - Answer -resources become depleted cell replication = cell
death
4.Death phase - Answer -1.Death phase - endospores can form cell replication <
cell death
Lag Phase - Answer -•Initial cell numbers do not change
•Cells grow larger; metabolically active
•Damaged or shocked cells undergo repair
•Duration of the lag phase determined by many factors including:
•Genetic make-up
•Media composition
•Initial inoculum size
©COPYRIGHT 2025, ALL RIGHTS RESERVED 3
