Electrophoresis - Answers__________ is a series of techniques by which biomolecules can be separated
based on size, length and/or shape.
Electrophoresis Learning System - AnswersYou will be using a new computer program this week called
ELS which stands for ________ ________ _______.
Length - AnswersThe fragments of DNA we will be examining this week in lab will be separated based on
fragment _____________.
Denature the DNA into single strands. - AnswersWhat is the primary purpose of heating your samples
prior to loading them to the gel?
autoradiography - AnswersThe final step in the ELS program is to "develop" your gel so that the DNA
fragments become visible. This technique uses a small amount of radioactive phosphorus and is called
___________.
false - AnswersThe DNA fragments used in this will exhibit a very simple mode of inheritance, which is
Simple Mendelian.
1;2 - AnswersBecause of the simple mode of inheritance it will be very easy to distinguish homozygotes
from heterozygotes during this lab exercise. Homozygotes will all show _______ band(s) and
heterozygotes will all show __________ band(s).
uncertainly exists in all scientific studies - AnswersThe Genotyping that we will be using in this exercise
provides us with an example of how science can help solve a problem. Genotyping also provides us with
another critical lesson, namely that ____________________.
Drosophila melanogaster - AnswersThe scientific (genus species) name for the fruitfly (or red-eyed
pomace fly) is?
8 - AnswersFruitflies have a total of _______ chromosomes.
XX, XY - AnswersFruit flies exhibit the Lygaeus Mode of Sex-determination. For each gender below, give
the correct chromosome assignment.
FEMALE ______
MALE ______
homozygous / homozygous - AnswersFor the Medelian option, the Fly Colony program is designed so
that the F1 progeny are all heterozygotes. This result simulates exactly what happens if we were
, crossing real flies and occurs because one Parent is _________ dominant while the other is selected to
be _________ recessive.
93 - AnswersA total of 372 offspring are produced. Some are red and some are blue. Given the cross that
you made, you expected a ratio of 3 red : 1 blue. How many blue offspring did you expect from the 372
progeny? (please enter your answer numerically in whole numbers, no decimals)
106 - AnswersA total of 424 offspring are produced. Some are red and some are blue. Given the cross
that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring did you expect from the
424 progeny? (please enter your answer numerically in whole numbers, no decimals)
963 - AnswersA total of 3852 offspring are produced. Some are red and some are blue. Given the cross
that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring did you expect from the
3852 progeny? (please enter your answer numerically in whole numbers, no decimals)
0.25 - AnswersIn a recent survey of students in General Genetics, 512 identified themselves are female
and 496 as male. Use Chi-Squared analysis to determine if this sample fits an expected ratio of 1:1. The
Observed Chi-Squared calculated from these data is ____?
14.58 - AnswersIn a recent survey of students in General Genetics, 511 identified themselves are female
and 396 as male. Use Chi-Squared analysis to determine if this sample fits an expected ratio of 1:1. The
Observed Chi-Squared calculated from these data is ____?
1.9 - AnswersIn a recent survey of students in General Genetics, 361 identified themselves are female
and 399 as male. Use Chi-Squared analysis to determine if this sample fits an expected ratio of 1:1. The
Observed Chi-Squared calculated from these data is ____?
DNA sequencing - Answers____________ is a technique for determining the precise order of the
nucleotides in a DNA fragment
polymerase chain reaction - AnswersPCR is a laboratory technique that allows geneticists to precisely
generate millions of copies of small fragments of DNA. The letters PCR are an abbreviation for
____________.
true - AnswersThe most commonly used sequencing technique was developed in 1977 by Dr. Fred
Sanger and is called Chain Termination sequencing.
di-deoxynucleotides - AnswersThe Sanger sequencing technique relies on 5 main components: DNA
template, a DNA primer, four deoxynucleoside triphosphates, DNA polymerase and ________________.
false - AnswersTo provide a common access point for all sequences, the National Institute of Health
created a free repository called GeneStore.
BLAST - AnswersTo determine the identity of your DNA samples, we will be using a search program
provided by the NIH (National Institute of Health). This program is called __________.